LncTUG1通过靶向miR-212-3p对口腔鳞状细胞癌细胞NK细胞杀伤敏感性的影响

doi:10.19439/j.sjos.2019.06.002

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (6): 567-571.doi: 10.19439/j.sjos.2019.06.002

LncTUG1通过靶向miR-212-3p对口腔鳞状细胞癌细胞NK细胞杀伤敏感性的影响

王培¹, 汤春波², 李斌¹, 傅宗云¹

1.东南大学附属中大医院口腔科,江苏南京 210009;
2.江苏省口腔医院口腔种植科,江苏南京 210029

收稿日期:2019-04-30 出版日期:2019-12-25 发布日期:2020-01-14
通讯作者: 王培,E-mail：1173792604@qq.com
作者简介:王培（1991-）,男,硕士,住院医师
基金资助:
国家自然科学基金（81470778）

Effect of LncTUG1 on NK cell killing sensitivity in oral squamous cell carcinoma cells by targeting miR-212-3p

WANG Pei¹, TANG Chun-bo², LI Bin¹, FU Zong-yun¹

1.Department of Stomatology, Zhongda Hospital Affiliated to Southeast University. Nanjing 210009;
2.Department of Dental Implantation, Jiangsu Stomatological Hospital. Nanjing 210029, Jiangsu Province, China

Received:2019-04-30 Online:2019-12-25 Published:2020-01-14

摘要/Abstract

摘要： 目的探讨长链非编码RNA（Lnc）牛磺酸调节基因1(taurine up-regulated gene 1,TUG1)对口腔鳞状细胞癌细胞自然杀伤细胞(nature killer cell, NK)杀伤敏感性的影响。方法以口腔鳞状细胞癌细胞作为体外实验对象,转染TUG1 siRNA,利用qRT-PCR、MTT、流式细胞术、LDH方法,分别检测TUG1表达量、细胞增殖、细胞凋亡和NK细胞杀伤率。利用生物信息学软件预测TUG1与miR-212-3p靶向互补结合,构建荧光素酶报告载体,鉴定靶向关系。在口腔鳞状细胞癌细胞中共转染TUG1 siRNA和miR-212-3p 抑制剂,评估miR-212-3p 抑制剂对TUG1 siRNA影响口腔鳞状细胞癌细胞增殖凋亡和NK细胞杀伤敏感性的影响。采用SPSS 21.0软件包对实验数据进行统计学分析。结果 TUG1 siRNA可显著降低口腔鳞状细胞癌细胞中TUG1的表达水平（P=0.000）,降低细胞增殖能力（P=0.001）,促进细胞凋亡（P=0.000）,增加NK细胞杀伤率（P<0.01）。TUG1 siRNA靶向提高miR-212-3p表达量。miR-212-3p 抑制剂可逆转TUG1 siRNA对口腔鳞状细胞癌细胞增殖、凋亡和NK细胞杀伤率的影响。结论下调TUG1可靶向负调控miR-212-3p,抑制口腔鳞状细胞癌细胞增殖并诱导凋亡,提高NK细胞杀伤敏感性。

关键词: 口腔鳞状细胞癌, NK细胞杀伤敏感性, miR-212-3p, TUG1

Abstract: PURPOSE: To investigate the effect of LncTUG1 on NK cell killing sensitivity in oral squamous cell carcinoma cells. METHODS: Oral squamous cell carcinoma cells were used as experimental objects in vitro. TUG1 siRNA was transfected,the expression of TUG1, cell proliferation, cell apoptosis and NK cell killing rate were detected by qRT-PCR, MTT, flow cytometry and LDH. Bioinformatics software was used to predict that TUG1 and miR-212-3p will target and complement each other, so luciferase reporter vector was constructed and the targeting relationship was identified. TUG1 siRNA and miR-212-3p inhibitor were co-transfected into oral squamous cell carcinoma cells, the effects of miR-212-3p inhibitor on TUG1 siRNA on proliferation, apoptosis and NK cell killing sensitivity of oral squamous cell carcinoma cells were evaluated. The data were analyzed with SPSS 21.0 software package. RESULTS: TUG1 siRNA could significantly reduce the expression of TUG1 in oral squamous cell carcinoma cells (P=0.000), decrease cell proliferation (P=0.001), promote cell apoptosis (P=0.000), increase the killing rate of NK cells (P<0.01). TUG1 siRNA targeted to increase the expression of miR-212-3p. miR-212-3p inhibitor could reverse the effects of TUG1 siRNA on proliferation, apoptosis and NK cell killing rate of oral squamous cell carcinoma cells. CONCLUSIONS: Down-regulation of TUG1 targeting and negative regulation of miR-212-3p inhibits proliferation, induces apoptosis and improves NK cell killing sensitivity of oral squamous cell carcinoma cells.

Key words: Oral squamous cell carcinoma, NK cell killing sensitivity, Mi-212-3p, TUG1

中图分类号:

R739.8

王培, 汤春波, 李斌, 傅宗云. LncTUG1通过靶向miR-212-3p对口腔鳞状细胞癌细胞NK细胞杀伤敏感性的影响[J]. 上海口腔医学, 2019, 28(6): 567-571.

WANG Pei, TANG Chun-bo, LI Bin, FU Zong-yun. Effect of LncTUG1 on NK cell killing sensitivity in oral squamous cell carcinoma cells by targeting miR-212-3p[J]. Shanghai Journal of Stomatology, 2019, 28(6): 567-571.

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LncTUG1通过靶向miR-212-3p对口腔鳞状细胞癌细胞NK细胞杀伤敏感性的影响

Effect of LncTUG1 on NK cell killing sensitivity in oral squamous cell carcinoma cells by targeting miR-212-3p

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