长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义

doi:10.19439/j.sjos.2019.01.013

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (1): 67-70.doi: 10.19439/j.sjos.2019.01.013

长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义

杨宗澄¹, 黄海燕², 徐欣^1,3

1. 山东大学口腔医学院种植科,山东济南 250012;
2. 安徽省立医院口腔科,安徽合肥 230001;
3. 山东省口腔组织再生重点实验室,山东济南 250012

收稿日期:2018-01-05 修回日期:2018-07-04 出版日期:2019-02-25 发布日期:2019-04-12
通讯作者: 徐欣, E-mail：xinxu@sdu.edu.cn
作者简介:杨宗澄(1991-),男,硕士研究生,E-mail：576825102@qq.com

Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance

YANG Zong-cheng¹, HUANG Hai-yan², XU Xin^1,3

1.Department of Dental Implant, Stomatological Hospital of Shandong University. Jinan 250012, Shandong Province;
2.Department of Stomatology, Anhui Provincial Hospital. Hefei 230001, Anhui Province;
3.Shandong Provincial Key Laboratory of Oral Tissue Regeneration. Jinan 250012, Shandong Province, China

Received:2018-01-05 Revised:2018-07-04 Online:2019-02-25 Published:2019-04-12

摘要/Abstract

摘要： 目的:探讨长链非编码RNA PCAT-1与口腔鳞状细胞癌发病的相关性。方法:收集2013年6月—2016年6月安徽省立医院口腔科收治的口腔鳞状细胞癌（OSCC）组织标本60例和非癌患者口腔组织标本52例,对OSCC患者标本中PCAT-1水平及其他临床特征（年龄、性别、高危习惯、组织分期、淋巴结转移、分化程度）进行分析;同时用RT-PCR检测标本中PCAT-1和c-Myc的表达水平,并分析其与口腔鳞状细胞癌发病的相关性。采用Graphpad Prism 7.0软件对数据进行统计学分析。结果:对60例口腔鳞状细胞癌标本结合临床信息进行分析,高危习惯（烟酒嗜好）组及淋巴结转移组PCAT-1水平显著高于无高危习惯组及无淋巴结转移组; PCAT-1和c-Myc表达水平显著高于非癌患者口腔组织标本的表达水平,且两者的表达呈正相关。结论:PCAT-1在口腔鳞状细胞癌中显著高表达,可能与口腔癌的发病有关。

关键词: 长链非编码RNA, PCAT-1, c-Myc, 口腔鳞状细胞癌

Abstract: PURPOSE: To investigate the correlation between the expression of lncRNA PCAT-1 and clinicopathological features of oral squamous cell carcinoma. METHODS: In this study, 112 oral tissue specimens, including 60 oral squamous cell carcinoma, and 52 non-cancer oral tissues were collected from Department of Stomatology in Anhui Provincial Hospital during 2013 to 2016. Reverse transcription quantitative PCR (RT-PCR) was performed to detect the expression of PCAT-1 and c-Myc. The correlation between the levels of PCAT-1 and clinical features (age, gender, high risk habit, histological stage, cervical lymphatic metastasis, differentiation) were analyzed. Graphpad Prism 7.0 software package was used for statistical analysis. RESULTS: Significant different levels of PCAT-1 were found between the high risk habit (alcohol and tobacco) group and low risk habit group, between patients with cervical lymphnode metastasis and patients without cervical lymphnode metastasis. The expression of PCAT-1 and c-Myc were up-regulated in OSCC, and there were positive correlation between the expression of PCAT-1 and c-Myc; up-regulated expression of PCAT-1 may be associated with higher morbidity of OSCC. CONCLUSIONS: PCAT-1 is overexpressed in oral squamous cell carcinoma and is associated with higher morbidity of OSCC.

Key words: Long non-coding RNA, PCAT-1, c-Myc, Oral squamous cell carcinoma

中图分类号:

R739.8

杨宗澄, 黄海燕, 徐欣. 长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义[J]. 上海口腔医学, 2019, 28(1): 67-70.

YANG Zong-cheng, HUANG Hai-yan, XU Xin. Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance[J]. Shanghai Journal of Stomatology, 2019, 28(1): 67-70.

参考文献

[1] Wu Z, Liu X, Liu L, et al.Regulation of lncRNA expression[J]. Cell Mol Biol Lett, 2014, 19(4): 561-575.
[2] Cui WC, Wu YF, Qu HM.Up-regulation of long non-coding RNA PCAT-1 correlates with tumor progression and poor prognosis in gastric cancer[J]. Eur Rev Med Pharmacol Sci, 2017, 21(13): 3021-3027.
[3] Qiao L, Liu X, Tang Y, et al.Down regulation of the long non-coding RNA PCAT-1 induced growth arrest and apoptosis of colorectal cancer cells[J]. Life Sci, 2017, 188: 37-44.
[4] Xu W, Chang J, Du X, et al.Long non-coding RNA PCAT-1 contributes to tumorigenesis by regulating FSCN1 via miR-145-5p in prostate cancer[J]. Biomed Pharmacother, 2017, 95: 1112-1118.
[5] Zhang D, Cao J, Zhong Q, et al.Long noncoding RNA PCAT-1 promotes invasion and metastasis via the miR-129-5p-HMGB1 signaling pathway in hepatocellular carcinoma[J]. Biomed Pharmacother, 2017, 95: 1187-1193.
[6] Ren Y, Shang J, Li J, et al.The long noncoding RNA PCAT-1 links the microRNA miR-215 to oncogene CRKL-mediated signaling in hepatocellular carcinoma[J]. J Biol Chem, 2017, 292(43): 17939-17949.
[7] Yang F, Xue X, Bi J, et al.Long noncoding RNA CCAT1, which could be activated by c-Myc, promotes the progression of gastric carcinoma[J]. J Cancer Res Clin Oncol, 2013, 139(3): 437-445.
[8] 单廷, 陈义钢, 洪波, 等. 长链非编码RNA PCAT-1在胃癌中的表达及临床意义[J]. 中华医学杂志, 2017, 97(18): 1411-1414.
[9] Deng L, Yang SB, Xu FF, et al.Long noncoding RNA PCAT-1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge[J]. J Exp Clin Cancer Res, 2015, 34: 18.
[10] He X, Tan X, Wang X, et al.C-Myc-activated long noncoding RNA PCAT-1 promotes colon cancer cell proliferation and invasion[J]. Tumour Boil, 2014, 35(12): 12181-12188.
[11] Zhang XF, Liu T, Li Y, et al.Overexpression of long non-coding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with breast cancer[J]. Int J Clin Exp Pathol, 2015, 8(8): 9440-9445.
[12] 韩勖, 凌志新, 胡强, 等. 长链非编码RNA PCAT-1对前列腺癌PC-3细胞增殖、迁移及凋亡的影响[J]. 东南大学学报(医学版), 2017, 36(1): 1-4.
[13] Lu L, Xu H, Luo F, et al.Epigenetic silencing of miR-218 by the lncRNA PCAT-1, acting via BMI1, promotes an altered cell cycle transition in the malignant transformation of HBE cells induced by cigarette smoke extract[J]. Toxicol Appl Pharmacol, 2016, 304: 30-41.
[14] Zhou B, Wang Y, Jiang J, et al.The long noncoding RNA colon cancer-associated transcript-1/miR-490 axis regulates gastric cancer cell migration by targeting hnRNPA1[J]. IUBMB Life, 2016, 68(3): 201-210.
[15] Chen L, Wang W, Cao L, et al.Long Non-Coding RNA PCAT-1 acts as a competing endogenous RNA to regulate cell growth and differentiation in acute myeloid leukemia[J]. Mol Cells, 2016, 39(4): 330-336.
[16] Chen C, Ridzon DA, Broomer AJ, et al.Realtime quantification of microRNAs by stem-loop RT-PCR[J]. Nucleic Acids Res, 2005, 33(20): e179.
[17] Kang K, Zhang X, Liu H, et al.A novel real-time PCR assay of microRNAs using S-Poly(T), a specific oligo(dT) reverse transcription primer with excellent sensitivity and specificity[J]. PLoS One, 2012, 7(11): e48536.
[18] Xiang JF, Yin QF, Chen T, et al.Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus[J]. Cell Res, 2014, 24(5): 513-531.
[19] Li J, Han L, Roebuck P, et al.An interactive open platform to explore the function of lncRNAs in cancer[J]. Cancer Res, 2015, 75(18): 3728-3737.
[20] Ge X, Chen Y, Liao X, et al.Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer[J]. Med Oncol, 2013, 30(2): 588.

长链非编码RNA PCAT-1在口腔鳞状细胞癌中的表达及临床意义

Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	赵娇, 隋佰延, 刘昕, 阮敏. 口腔鳞状细胞癌免疫相关预后风险模型的构建与验证[J]. 上海口腔医学, 2024, 33(4): 345-353.
[2]	刘文静, 李梦琦, 崔祥, 汪俊兰. circ_0000326靶向miR-567对口腔鳞状细胞癌HSC3细胞增殖、侵袭及迁移的调控效应[J]. 上海口腔医学, 2023, 32(6): 590-596.
[3]	陈兴金, 范然怡, 陶识丞, 薛睿, 文旭涛, 麦华明. 颈淋巴清扫术保留胸锁乳突肌与否对晚期口腔鳞癌预后的影响[J]. 上海口腔医学, 2023, 32(6): 609-614.
[4]	叶婷婷, 陈蔚华, 裴婧, 贾云香, 吴海莹. NEK2与EMT相关分子在口腔鳞状细胞癌中的表达及临床意义[J]. 上海口腔医学, 2023, 32(6): 640-644.
[5]	陈娟, 曹若妍, 刘洋, 洪鹏宇, 唐瞻贵. TNFAIP6在口腔疣状癌与口腔鳞状细胞癌中的表达及其与临床病理的关系[J]. 上海口腔医学, 2022, 31(2): 167-172.
[6]	靳能皓, 南欣荣, 闫星泉. BTG1在78例口腔鳞癌中的表达及临床意义[J]. 上海口腔医学, 2021, 30(2): 140-144.
[7]	王科, 彭国光, 谭玉莲, 何善志, 罗翠芬. miR-99a对口腔鳞癌增殖能力的影响[J]. 上海口腔医学, 2021, 30(1): 44-49.
[8]	黄丹妮, 陈文鑫, 熊浩峰, 胡鑫, 毛婷, 苏彤. 口腔鳞状细胞癌患者术后服用二甲双胍对预后的影响[J]. 上海口腔医学, 2021, 30(1): 61-65.
[9]	郑晶, 李天客, 包阳, 张素欣. Spindly和Bub3在口腔鳞状细胞癌中的表达及意义[J]. 上海口腔医学, 2020, 29(5): 528-532.
[10]	达雨, 杨震, 夏苗苗, 王梦佳. Ki-67、PI3K、Beclin1在口腔鳞癌组织中的表达及意义[J]. 上海口腔医学, 2020, 29(1): 75-79.
[11]	王培, 汤春波, 李斌, 傅宗云. LncTUG1通过靶向miR-212-3p对口腔鳞状细胞癌细胞NK细胞杀伤敏感性的影响[J]. 上海口腔医学, 2019, 28(6): 567-571.
[12]	涂敏松, 李逸松, 张雄. YAP、TAZ蛋白在口腔鳞状细胞癌中的表达及意义[J]. 上海口腔医学, 2018, 27(4): 415-418.
[13]	李少明, 高岭, 任文豪, 薛令法, 许尧祥, 岳金, 郅克谦. 功能性与根治性颈淋巴清扫术在早期口腔鳞状细胞癌治疗中的疗效比较[J]. 上海口腔医学, 2017, 26(3): 305-308.
[14]	吴宁, 陆瑛, 梁继宗. Survivin和hsa-miR-542-3p在口腔鳞状细胞癌中的表达和相关性分析[J]. 上海口腔医学, 2016, 25(6): 720-724.
[15]	蒋丽兰, 赵娅军, 李勇. TGF-βRⅡ和NF-κB在口腔鳞状细胞癌的表达及临床意义[J]. 上海口腔医学, 2016, 25(6): 729-733.