ANXA1对口腔鳞癌TPF化疗敏感性的影响及作用机制探讨

doi:10.19439/j.sjos.2019.03.001

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (3): 225-230.doi: 10.19439/j.sjos.2019.03.001

ANXA1对口腔鳞癌TPF化疗敏感性的影响及作用机制探讨

朱东旺^*, 孙文文^*, 赵铜超, 钟来平, 张志愿

上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2019-02-18 修回日期:2019-04-24 出版日期:2019-06-25 发布日期:2019-08-09
通讯作者: 钟来平,E-mail：zhonglaiping@163.com
作者简介:朱东旺（1986-）,男,博士,住院医师,E-mail：zhudongwang@hotmail.com;孙文文（1990-）,女,硕士,住院医师,E-mail：sunwenwen9th@126.com。^*并列第一作者
基金资助:
国家自然科学基金（81602370）

The effect and mechanism of ANXA1 on TPF chemosensitivity in oral squamous cell carcinoma

ZHU Dong-wang, SUN Wen-wen, ZHAO Tong-chao, ZHONG Lai-ping, ZHANG Zhi-yuan

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011, China;

Received:2019-02-18 Revised:2019-04-24 Online:2019-06-25 Published:2019-08-09

摘要/Abstract

摘要： 目的探讨ANXA1在口腔鳞癌TPF化疗中的作用机制。方法构建ANXA1过表达及低表达细胞系,通过细胞增殖、细胞毒性实验、实时荧光定量PCR和Western免疫印迹方法,分析ANXA1在TPF化疗中的作用,并探讨ANXA1通过上皮-间质转化（EMT）通路在TPF化疗中的作用机制。采用SPSS18.0软件包对数据进行统计学分析。结果过表达口腔鳞癌细胞系ANXA1后,细胞生长速率降低,细胞周期减慢,对TPF诱导化药物敏感性降低,口腔鳞癌细胞出现EMT;低表达口腔鳞癌细胞系ANXA1后,细胞生长速率增快,细胞周期加快,对TPF化疗药物敏感性增加,口腔鳞癌细胞出现逆EMT。结论在口腔鳞癌TPF化疗中,ANXA1过表达导致细胞出现EMT,对化疗敏感性降低。

关键词: 口腔鳞癌, 膜联蛋白A1, 上皮-间质转化, 化疗敏感性

Abstract: PURPOSE: To investigate the mechanism of ANXA1 in TPF chemotherapy of oral squamous cell carcinoma (OSCC). Methods: ANXA1 overexpression and low-expression cell lines were constructed. The role of ANXA1 in TPF chemotherapy was analyzed by cell proliferation, cytotoxicity test, real-time PCR and Western blot, and the mechanism of ANXA1 in TPF chemotherapy through EMT (epithelial-mesenchymal transition) pathway was discussed. The data were analyzed with SPSS 18.0 software package. Results: After overexpression of ANXA1, cell growth rate decreased, cell cycle slowed down, sensitivity to TPF-induced drugs decreased, and EMT occurred in OSCC. After underexpression of ANXA1, cell growth rate increased, cell cycle accelerated, sensitivity to TPF chemotherapeutic drugs increased, and reverse EMT occurred in OSCC. Conclusions: In TPF chemotherapy of OSCC, overexpression of ANXA1 results in EMT of cells, which leads to decreased chemosensitivity.

Key words: Oral squamous cell carcinoma, ANXA1, EMT, Chemosensitivity

中图分类号:

R739.8

朱东旺, 孙文文, 赵铜超, 钟来平, 张志愿. ANXA1对口腔鳞癌TPF化疗敏感性的影响及作用机制探讨[J]. 上海口腔医学, 2019, 28(3): 225-230.

ZHU Dong-wang, SUN Wen-wen, ZHAO Tong-chao, ZHONG Lai-ping, ZHANG Zhi-yuan. The effect and mechanism of ANXA1 on TPF chemosensitivity in oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2019, 28(3): 225-230.

参考文献

[1] Kademani D.Oral cancer[J]. Mayo Clin Proc, 2007, 82(7): 878-887.
[2] Petersen PE.The World Oral Health Report 2003: continuous improvement of oral health in the 21st century-the approach of WHO Global Oral Programme[J]. Community Dent Oral Epidemiol, 2003, 31(Suppl 1): 3-23.
[3] Neville BW, Day TA.Oral cancer and precancerous lesions[J]. CA Cancer J Clin, 2002, 52(4): 195-215.
[4] Parkin DM, Bray F, Ferlay J, et al.Global cancer statistics, 2002[J]. CA Cancer J Clin, 2005, 55(2): 74-108.
[5] Zhong L, Zhang C, Ren G, et al.Long-term results of a randomized phase III trial of TPF induction chemotherapy followed by surgery and radiation in locally advanced oral squamous cell carcinoma[J]. Oncotarget, 2015, 6(21): 18707-18714.
[6] Zhu DW, Liu Y, Yang X, et al.Low Annexin A1 expression predicts benefit from induction chemotherapy in oral cancer patients with moderate or poor pathologic differentiation grade[J]. BMC Cancer, 2013, 13: 301.
[7] Faria PC, Sena AA, Nascimento R, et al.Expression of annexin A1 mRNA in peripheral blood from oral squamous cell carcinoma patients[J]. Oral Oncol, 2010, 46(1): 25-30.
[8] Wang LP, Bi J, Yao C, et al.Annexin A1 expression and its prognostic significance in human breast cancer[J]. Neoplasma, 2010, 57(3): 253-259.
[9] de Graauw M, van Miltenburg MH, Schmidt MK, et al. Annexin A1 regulates TGF-beta signaling and promotes metastasis formation of basal-like breast cancer cells[J]. Proc Natl Acad Sci USA, 2010, 107(14): 6340-6345.
[10] Maschler S, Gebeshuber CA, Wiedemann EM, et al.Annexin A1 attenuates EMT and metastatic potential in breast cancer[J]. EMBO Mol Med, 2010, 2(10): 401-414.
[11] Fischer KR, Durrans A, Lee S, et al.EMT is not required for lung metastasis but contributes to chemoresistance[J]. Nature, 2015, 527(7579): 472-476.
[12] Zheng X, Carstens JL, Kim J, et al.Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer[J]. Nature, 2015, 527(7579): 525-530.
[13] Guo C, Liu S, Sun MZ.Potential role of Anxa1 in cancer[J]. Future Oncol, 2013, 9(11): 1773-1793.
[14] Li S, Zhang XM, Zhang RK, et al.Hippo pathway contributes to cisplatin resistant-induced EMT in nasopharyngeal carcinoma cells[J]. Cell Cycle, 2017, 16(17): 1601-1610.

[1]	谢玉婷, 熊屏. 基于单原子铁纳米催化剂的纳米催化疗法对口腔鳞癌细胞Cal27的作用[J]. 上海口腔医学, 2024, 33(2): 117-122.
[2]	段咏华, 吴开柳, 梅健, 周谊渊, 刘梦石. 口腔鳞癌组织中RACK1与EGP40的表达及其与临床病理特征及预后的相关性[J]. 上海口腔医学, 2023, 32(4): 385-390.
[3]	董淋升, 唐进, 程维, 冉婧璇, 刘兆博, 李雅冬. IL-6和β-catenin在口腔鳞癌中的表达及预后价值[J]. 上海口腔医学, 2023, 32(3): 280-286.
[4]	胡小媛, 王宁, 张晓野, 杨茜, 高菲, 王成勤, 尚伟, 项锋钢, 冯元勇. Chemerin对口腔鳞癌中性粒细胞浸润的影响及机制探讨[J]. 上海口腔医学, 2023, 32(2): 158-165.
[5]	郭梦瑶, 李凯玉, 聂敏海, 刘旭倩. 上调Decorin对口腔鳞癌荷瘤裸鼠EGFR、C-Myc、p21表达的影响[J]. 上海口腔医学, 2023, 32(1): 40-46.
[6]	文旭涛, 马振, 张翀, 陶识丞, 陈兴金, 麦华明. 不同培养条件对CD133⁺原代口腔鳞癌细胞干性维持的影响[J]. 上海口腔医学, 2022, 31(4): 343-348.
[7]	秦志明, 揭伟萍, 池彦廷, 李宏锋, 李斌斌. 基于局部可变阈值的无监督定量Ki-67细胞增殖指数模型的初步建立[J]. 上海口腔医学, 2022, 31(3): 248-254.
[8]	买尔哈巴·买合木提, 拜婕, 如斯坦木·依米提, 姚志涛. 50例口腔鳞癌患者治疗过程中营养状况改变及影响因素分析[J]. 上海口腔医学, 2022, 31(2): 205-210.
[9]	谈亦然, 赵铜超, 琚梧桐, 钟来平. 长春新碱对口腔鳞癌中Stathmin调控作用的实验研究[J]. 上海口腔医学, 2022, 31(1): 1-5.
[10]	谢非, 秦星, 童桐, 蒋英英, 张建军. IGF2BP1在口腔鳞癌细胞耐药中的作用及机制探讨[J]. 上海口腔医学, 2021, 30(5): 456-461.
[11]	李蕾, 李晓东, 庄乾伟, 葛良玉, 李志萍, 顾倩平, 白玉婷, 孟箭. 超声热疗联合TPF化疗治疗19例老年晚期口腔鳞癌的疗效分析[J]. 上海口腔医学, 2021, 30(5): 543-547.
[12]	邵小钧, 朱乔, 刘有, 韩小东, 林小臻, 席庆. 咀嚼槟榔与口腔鳞癌的相关临床病理学因素分析[J]. 上海口腔医学, 2021, 30(3): 268-272.
[13]	薛佳艺, 朱晓燕, 危常磊, 孙培, 张慧, 邓婧. 25例口腔鳞癌患者唾液代谢组学分析[J]. 上海口腔医学, 2021, 30(2): 201-205.
[14]	王树斌, 秦帅华, 易雅群, 李新明. 216例口腔鳞癌患者术后心理困扰影响因素及放疗前、后生存质量评价[J]. 上海口腔医学, 2021, 30(1): 71-76.
[15]	傅宗云, 陶峰. MMP-14、ING4和HIF-1α在60例口腔鳞癌中的表达及临床意义[J]. 上海口腔医学, 2020, 29(6): 642-646.

ANXA1对口腔鳞癌TPF化疗敏感性的影响及作用机制探讨

The effect and mechanism of ANXA1 on TPF chemosensitivity in oral squamous cell carcinoma

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价