尼妥珠单抗对 125I粒子照射人舌鳞癌CAL-27细胞的增敏作用

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (4): 409-413.

尼妥珠单抗对 ¹²⁵I粒子照射人舌鳞癌CAL-27细胞的增敏作用

王兴, 辜健敏, 孟箭, 邵翠玲

徐州市中心医院口腔科,江苏徐州 221009

收稿日期:2015-12-07 出版日期:2016-08-25 发布日期:2016-09-06
通讯作者: 孟箭,E-mail: mrocket@126.com.
作者简介:王兴(1986-),男,硕士研究生,住院医师,
基金资助:
江苏省“六大人才高峰”D类资助项目（2012-WS-099）; 徐州市医学创新（技术攻关）团队（xwcx201604）

The radiosensitization of nimotuzumab on CAL-27 cells after ¹²⁵I seeds irradiation

WANG Xing, GU Jian-min, MENG Jian, SHAO Cui-ling

Department of Stomatology, Central Hospital of Xuzhou City. Xuzhou 221009, Jiangsu Province, China

Received:2015-12-07 Online:2016-08-25 Published:2016-09-06

摘要/Abstract

摘要： 目的观察尼妥珠单抗联合¹²⁵I粒子持续低剂量照射对人舌鳞癌CAL-27细胞的抑制效果,初步探讨尼妥珠单抗对¹²⁵I粒子照射人舌鳞癌CAL-27细胞的放射增敏作用。方法将指数生长期CAL-27细胞随机分为4组,（空白对照组、¹²⁵I粒子照射组、尼妥珠单抗组和尼妥珠单抗联合¹²⁵I粒子照射组）,采用CCK-8法检测¹²⁵I粒子照射组对CAL-27细胞的抑制情况,流式细胞技术测定各组细胞周期分布及细胞凋亡率,Hoechst 33258染色比较观察细胞的形态学变化。采用SPSS17.0软件包对数据进行统计学分析。结果¹²⁵I粒子对CAL-27细胞生长有抑制作用且呈时间剂量依赖性。尼妥珠单抗联合¹²⁵I粒子照射组CAL-27细胞凋亡率显著高于单纯尼妥珠单抗组和单纯照射细胞凋亡率之和,联合组S期细胞比例较对照组显著减少(P<0.05)。结论尼妥珠单抗联合¹²⁵I粒子照射可以通过诱导凋亡的途径杀伤CAL-27细胞,且尼妥珠单抗与¹²⁵I粒子照射释放的低剂量γ射线照射CAL-27细胞具有明显的放射增敏作用。

关键词: 尼妥珠单抗, 舌鳞状细胞癌, 125I粒子, 表皮生长因子, 放射增敏

Abstract: PURPOSE: To observe the inhibitory effect of nimotuzumab combined with low-dose continuous irradiation using ¹²⁵I seeds on CAL-27 cells originating from human tongue squamous carcinoma, and explore the radiosensitization of nimotuzumab on human tongue squamous carcinoma CAL-27 irradiated with ¹²⁵I seeds. METHODS: The exponential-phase CAL-27 cells were randomly divided into 4 groups: control group, ¹²⁵I seeds irradiation group, nimotuzumab group, nimotuzumab plus ¹²⁵I seeds irradiation group. CCK-8 assay was used to detect the inhibitory effect of ¹²⁵I seeds irradiation on CAL-27 cancer cells, and flow cytometry assays were performed to calculate the apoptosis rate of cells and cell cycle from each group. The morphology of cells was compared using Hoechst 33258 staining. The data were analyzed with SPSS17.0 software package. RESULTS: ¹²⁵I seeds inhibited the growth of CAL-27 cells in time-dose dependent manner. The apoptosis rate of cells irradiated by nimotuzumab combined with ¹²⁵I seeds irradiation was higher than that treated with nimotuzumab and ¹²⁵I seeds irradiation respectively, and nimotuzumab followed by radiation had a lower S phase cells(P<0.05). CONCLUSIONS: Nimotuzumab combined with ¹²⁵I seeds irradiation killed CAL-27 cells by inducing apoptosis. Nimotuzumab and low-dose γ ray emitted from ¹²⁵I seeds can significantly enhance the radiosensitivity of CAL-27.

Key words: Nimotuzumab, Tongue squamous cell carcinoma, 125I seeds, Epidermal growth factor receptor, Radiosensitization

中图分类号:

R739.8

王兴, 辜健敏, 孟箭, 邵翠玲. 尼妥珠单抗对 ¹²⁵I粒子照射人舌鳞癌CAL-27细胞的增敏作用[J]. 上海口腔医学, 2016, 25(4): 409-413.

WANG Xing, GU Jian-min, MENG Jian, SHAO Cui-ling. The radiosensitization of nimotuzumab on CAL-27 cells after ¹²⁵I seeds irradiation[J]. Shanghai Journal of Stomatology, 2016, 25(4): 409-413.

参考文献

[1] Vernham GA, Crowther JA. Head and neck carcinoma-stage at presentation[J]. Clin Otolaryngol Allied Sci, 1994, 19(2): 120-124.
[2] Dirix P, Nuyts S. Evidence-based organ-sparing radiotherapy in head and neck cancer [J]. Lancet Oncol, 2010, 11(1): 85-91.
[3] Meng J, Meng QF, Gu QP, et al. Radioactive seed implantation combined with prosthesis denture in treatment of oral and maxillofacial malignancy [J].Cell Biochem Biophys, 2013, 67(3): 1529-1532.
[4] 张杰, 郑磊, 黄民伟, 等. 放射性粒子组织间植入治疗口腔颌面-头颈部腺源性恶性肿瘤近期临床疗效分析 [J]. 北京大学学报医学版, 2011, 43(2): 272-275.
[5] Meng J, Gu QP, Meng QF, et al. Efficacy of nimotuzumab combined with docetaxel-cisplatin-fluorouracil regimen in treatment of advanced oral caricinoma [J]. Cell Biochem Biophys, 2014, 68(1): 181-184.
[6] Hayes DN, Raez LE, Sharma AK, et al. Multicenter randomized phase II trial of combined radiotherapy and cisplatin with or without erlotinib in patients with locally advanced squamous cell carcinoma of the head and neck (SCCAHN): preliminary toxicity results [J]. J Clin Oncol, 2010, 28(15_Suppl): 5580.
[7] 王秀力, 孟箭. 尼妥珠单抗联合顺铂对人舌鳞状细胞癌细胞株CAL-27的抑制作用 [J]. 中华口腔医学研究杂志(电子版), 2013, 7(1): 15-17.
[8] Jatin PS. Cancer of the head and neck (American cancer society atlas of oncology) [M]. London: Bc Decker Inc., 2001: 1-4.
[9] 邱蔚六. 口腔颌面外科学[M]. 第6版. 北京: 人民卫生出版社, 2010: 274-275.
[10] 王俊杰, 修典荣, 冉维强. 放射性粒子组织间近距离治疗肿瘤[M]. 第2版. 北京: 北京大学医学出版社, 2004: 26-61.
[11] Mirzaie-Joniani H, Eriksson D, Sheikholvaezin A, et al. Apoptosis induced by low-dose and low-dose-rate radiation [J]. Cancer, 2002, 94(4 Suppl): 1210-1214.
[12] Zhuang HQ, Wang JJ, Liao AY, et al. The biological effect of 125 I seed continuous low dose rate irradiation in CL187 cells[J]. J Exp Clin Cancer Res, 2009, 28: 12.
[13] Shrivastav M, De Haro LP, Nickoloff JA. Regulation of DNA double-strand break repair pathway choice [J]. Cell Res, 2008, 18(1): 134-147.
[14] Liang K, Ang KK, Milas L, et al. The epidermal growth factor receptor mediates radioresistance [J]. Int J Radiat Oncol Biol Phys, 2003, 57(1): 246-254.
[15] Bianco C, Giovannetti E, Ciardiello F, et al. Synergistic antitumor activity of ZD6474, an inhibitor of vascular endothelial growth factor receptor and epidermal growth factor receptor signaling, with gemcitabine and ionizing radiation against pancreatic cancer [J]. Clin Cancer Res, 2006, 12(23): 7099-7107.
[16] Burdak-Rothkamm S, Rübe CE, Nguyen TP, et al. Radiosensitivity of tumor cell lines after pretreatment with the EGFR tyrosine kinase inhibitor ZD1839 (Iressa) [J]. Strahlenther Oncol, 2005, 181(3): 197-204.
[17] Rodríguez MO, Rivero TC, del Castillo Bahi R, et al. Nimotuzumab plus radiotherapy for unresectable squamous-cell carcinoma of the head and neck[J]. Cancer Biol Ther, 2010, 9(5): 343-349.
[18] Numico G, Franco P, Cristofano A, et al. Is the combination of Cetuximab with chemo-radiotherapy regimens worthwhile in the treatment of locally advanced head and neck cancer? A review of current evidence [J]. Crit Rev Oncol Hematol, 2013, 85(2): 112-120.

尼妥珠单抗对 ¹²⁵I粒子照射人舌鳞癌CAL-27细胞的增敏作用

The radiosensitization of nimotuzumab on CAL-27 cells after ¹²⁵I seeds irradiation

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

[1]	郭梦瑶, 李凯玉, 聂敏海, 刘旭倩. 上调Decorin对口腔鳞癌荷瘤裸鼠EGFR、C-Myc、p21表达的影响[J]. 上海口腔医学, 2023, 32(1): 40-46.
[2]	李羽, 黄文青, 陈林林. LncRNA NEAT1通过miR-339-5p/ITGA3轴调控舌鳞状细胞癌增殖、迁移及侵袭的分子机制[J]. 上海口腔医学, 2020, 29(3): 267-274.
[3]	周璐, 龚霞, 熊屏. 载药脂质体-微泡复合物超声导向治疗头颈鳞癌的体外实验研究[J]. 上海口腔医学, 2020, 29(2): 168-173.
[4]	印志法, 王思明. rhEGF对口腔颌面部外伤患者血清EGF及炎症因子水平的影响[J]. 上海口腔医学, 2017, 26(6): 654-657.
[5]	袁昌青, 曹颖秀, 刘斌, 董静, 邓婧, 耿国梁. 表皮生长因子与干燥综合征伴发萎缩性舌炎的相关性研究[J]. 上海口腔医学, 2014, 23(6): 731-735.
[6]	李丹, 向彬, 英晓霞, 董会. 舌鳞状细胞癌中EphA7的表达及其与患者病理参数和预后的相关分析[J]. 上海口腔医学, 2014, 23(5): 575-579.
[7]	赵民;付秀兰;吕红. 表皮生长因子受体在口腔扁平苔藓、口腔乳头瘤及口腔鳞状细胞癌中的表达 [J]. 上海口腔医学, 2012, 21(6): 673-676.