葡萄籽原花青素对牙龈卟啉单胞菌内毒素的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 443-446.

葡萄籽原花青素对牙龈卟啉单胞菌内毒素的影响

慈向科, 陈丽培, 欧晓艳

南昌大学附属口腔医院预防科,江西南昌 330006

收稿日期:2014-06-18 出版日期:2015-08-20 发布日期:2015-09-10
作者简介:慈向科（1985-）,女,硕士研究生, 电话：021-63121780,53315271,传真：021-63121780。
基金资助:
江西省南昌市科技局科研计划项目

Grape seed proanthocyanidin extracts inhibit lipopolysaccharide of Porphyromonas gingivalis

CI Xiang-ke, CHEN Li-pei, OU Xiao-yan

Department of Preventive Dentistry, Affiliated Stomatological Hospital of Nanchang University. Nanchang 330006, Jiangxi Province, China

Received:2014-06-18 Online:2015-08-20 Published:2015-09-10
Supported by:
Supported by Research Project of Bureau of Science and Technology of Nanchang City, Jiangxi Province (2014-80)

摘要/Abstract

摘要： 目的探讨葡萄籽原花青素对牙龈卟啉单胞菌内毒素的影响。方法采用二倍稀释法测定葡萄籽原花青素对牙龈卟啉单胞菌的最小抑菌浓度(MIC);采用鲎试验测定低于MIC 6个浓度的葡萄籽原花青素对牙龈卟啉单胞菌内毒素含量的影响。采用SPSS17.0软件包进行组间比较。结果葡萄籽原花青素对牙龈卟啉单胞菌的MIC为0.8 mg/mL;在0.05~0.4 mg/mL范围内,随着葡萄籽原花青素浓度的升高,对释放内毒素的抑制作用逐渐增强（P<0.05、0.01）。结论葡萄籽原花青素对牙龈卟啉单胞菌内毒素的释放具有抑制作用。

关键词: 葡萄籽原花青素, 牙龈卟啉单胞菌, 内毒素

Abstract: PURPOSE: To study the inhibitory effect of grape seed proanthocyanidin extracts on 1ipopolysacharides of Porphyromonas gingivalis. METHODS: Grape seed proanthocyanidin extracts were to different concentrations by dilution. The effect on Porphyromonas gingivalis was measured by minimal inhibitory concentration (MIC). The inhibition effect of grape seed proanthocyanidin extracts on lipopolysaccharide was measured by limulus assay with 6 concentrations below the minimal inhibitory concentration. SPSS 17.0 software package was used for statistical analysis. RESULTS: The value of MIC of grape seed proanthocyanidin extracts was 0.8 mg/mL to the strains of the experimental bacteria. The inhibitory effect on lipopolysaccharide enhanced with the increasing of the concentration of grape seed proanthocyanidin extracts within the scope of 0.05 to 0.4 mg/mL (P<0.05,0.01). CONCLUSIONS: Grape seed proanthocyanidin extracts have inhibitory effect on lipopolysaccharide of Porphyromonas gingivalis.

Key words: Grape seed proanthocyanidin extracts, Porphyromonas gingivalis, Lipopolysaccharide

中图分类号:

R781

慈向科, 陈丽培, 欧晓艳. 葡萄籽原花青素对牙龈卟啉单胞菌内毒素的影响[J]. 上海口腔医学, 2015, 24(4): 443-446.

CI Xiang-ke, CHEN Li-pei, OU Xiao-yan. Grape seed proanthocyanidin extracts inhibit lipopolysaccharide of Porphyromonas gingivalis[J]. Shanghai Journal of Stomatology, 2015, 24(4): 443-446.

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