丹参对大鼠正畸牙牙周组织TGF-β1表达的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (3): 288-293.

丹参对大鼠正畸牙牙周组织TGF-β₁表达的影响

张疆弢,梅梅,李平,张跃蓉,江策,丰雷,黄瑾,王家佳

遵义医学院附属口腔医院正畸科,贵州遵义 563003

收稿日期:2014-08-11 出版日期:2015-06-20 发布日期:2015-07-24
通讯作者: 张疆弢,Tel:0852-28635725,E-mail:zhangjiangtao75@sina.com
作者简介:张疆弢（1975-）,女,硕士,副主任医师
基金资助:
遵义市红花岗区科学技术资助项目[合社字(2010)11号]

The influence of salvia miltiorhiza on the expression of TGF-β₁in periodontal tissue during orthodontic tooth movement in rats

ZHANG Jiang-tao,MEI Mei,LI Ping,ZHANG Yue-rong,JIANG Ce,FENG Lei,HUANG Jin,WANG Jia-jia

Department of Orthodontic, Zunyi Medical College Affiliated Stomatology Hospital. Zunyi 563003, Guizhou Province, China

Received:2014-08-11 Online:2015-06-20 Published:2015-07-24
Supported by:
Research Fund of Science and Technology of Honghuagang District, Zunyi City[(2010)11]

摘要/Abstract

摘要： 目的:观察丹参注射液对大鼠正畸牙牙周组织TGF-β₁蛋白的表达变化。方法:选取SD雄性大鼠192只,随机分为丹参+加力组(A)、单纯加力组(B)、丹参对照组(C)和空白对照组(D)。A、B 2组再根据加力时间点建立正畸牙移动模型。A、C 2组与B、D 2组隔天于左侧上颌第一磨牙颊侧黏膜下分别注射丹参注射液及生理盐水。于加力后l、3、5、7、10、14 d分批处死大鼠,收集标本。体式显微镜测量大鼠正畸牙移动距离的改变,H-E染色观察牙周组织变化,免疫组织化学染色检测TGF-β₁蛋白的表达变化,采用SPSS17.0软件包对数据进行统计学分析。结果:除第1天外,A组TGF-β₁的表达均高于其余组别,3 d时TGF-β₁染色显著增强,5 d达高峰;与B组相比,第3、5、7天时间点TGF-β₁平均吸光度值分别为0.5181±0.0037、0.5857±0.0023和0.4363±0.0021,均具有显著差异（P<0.01）;与C组及D组相比,任何时间点均有显著差异（P<0.05）。结论:丹参注射液可通过改善牙周组织微循环,进而促进TGF-β₁蛋白的表达,可能是加速正畸牙移动的作用机制之一。

关键词: 丹参, 转化生长因子β, 1, 正畸牙, 牙周组织

Abstract: PURPOSE: To investigate the effect of salvia miltiorhiza on the expression of TGF-β₁ within periodontal tissues during orthodontic tooth movement in rat. METHODS: One hundred and ninety-two male Sprague-Dawley rats were randomly divided into 4 groups (group A, B, C and D). Group A and B were used as orthodontic tooth movement model based on afterburner time. Group A, C and B, D were injected salvia miltiorhiza and NS under the buccal mucosa of left maxillary first molar every other day. Animals in each group were sacrificed at 6 time points（day 1,3,5,7,10 and 14) after appliance placement for collection of specimens. The distance of the tooth movement was measured by stereomicroscope, periodontal tissue was investigated by H-E staining and the protein expression of TGF-β₁ was examined by immunohistochemical techniques. SPSS17.0 software package was used for statistical analysis. RESULTS: Except for the first day, the protein expression of TGF-β₁in group A was highest and there was significant up-regulation on day 3, with a peak on day 5. Compared with group B, the average optical density of TGF-β₁ in group A was 0.5181±0.0037 on day 3, 0.5857±0.00235 on day 5 and 0.4363±0.0021 on day 7, both with significant differences (P<0.01). Compared with group C and group D, significant differences were formed at each time point in group A(P<0.05). CONCLUSIONS: Salvia injection may improve periodontal tissue microcirculation,thus promote the expression of TGF-β₁ protein,which may be one of the mechanisms to accelerate orthodontic tooth movement.

Key words: Salvia miltiorhiza, TGF-β1, Orthodontics, Periodontal tissue

中图分类号:

R783.5

张疆弢,梅梅,李平,张跃蓉,江策,丰雷,黄瑾,王家佳. 丹参对大鼠正畸牙牙周组织TGF-β₁表达的影响[J]. 上海口腔医学, 2015, 24(3): 288-293.

ZHANG Jiang-tao,MEI Mei,LI Ping,ZHANG Yue-rong,JIANG Ce,FENG Lei,HUANG Jin,WANG Jia-jia. The influence of salvia miltiorhiza on the expression of TGF-β₁in periodontal tissue during orthodontic tooth movement in rats[J]. Shanghai Journal of Stomatology, 2015, 24(3): 288-293.

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丹参对大鼠正畸牙牙周组织TGF-β₁表达的影响

The influence of salvia miltiorhiza on the expression of TGF-β₁in periodontal tissue during orthodontic tooth movement in rats

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