IL-1β对人牙龈成纤维细胞在不同钛板表面表达炎性因子的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (2): 151-156.

IL-1β对人牙龈成纤维细胞在不同钛板表面表达炎性因子的影响

孙志新,张云涛

滨州医学院附属医院口腔修复科,山东滨州 256603

收稿日期:2014-07-02 出版日期:2015-04-20 发布日期:2015-07-24
通讯作者: 张云涛,E-mail:zytao999@163.com
作者简介:孙志新(1990-),女,硕士,E-mail:544242395@qq.com
基金资助:
山东省科学技术发展计划 (2011Y121008)

Effect of IL-1β on inflammatory factors synthesis of human gingival fibroblasts attached to different titaniums

SUN Zhi-xin,ZHANG Yun-tao

Department of Prosthodontic, Affiliated Hospital to Binzhou Medical College. Binzhou 256603, Shandong Province, China

Received:2014-07-02 Online:2015-04-20 Published:2015-07-24
Supported by:
Science and Technology Development Plan of Shandong Province (2011Y121008)

摘要/Abstract

摘要： 目的探讨白介素 1β对不同钛板表面牙龈成纤维细胞分泌炎性因子的影响。方法将牙龈成纤维细胞接种在3种不同表面结构组分的钛板上,在白介素 1β的刺激下,在3、6、24 h分别用聚合酶链反应（PCR）检测炎性因子白介素6及白介素8的表达量。采用SPSS13.0软件包对数据进行统计学分析。结果在白介素1β的诱导下,牙龈成纤维细胞对炎性因子（IL-6、IL-8）的表达量明显增加;纯钛与钛合金的钛板比表面氮化处理的钛板产生炎性因子（IL-6、IL-8）的表达量较少。结论白介素1β能刺激炎性因子的高表达;纯钛和钛合金IL-6、IL-8表达量较少。采用纯钛和钛合金种植体,可减少种植体周围炎的发生率,提高种植成功率。

关键词: 牙龈成纤维细胞, 白介素 1β, 白介素 6, 白介素 8

Abstract: PURPOSE: To evaluate the effects of IL-1β on inflammatory factors synthesis of human gingival fibroblasts on different surfaces of titaniums in vitro. METHODS: Human gingival fibroblasts were cultured on 3 different kinds of titaniums. The cells were stimulated with IL-1β, and the IL-6 and IL-8 production was detected by polymerase chain reaction (PCR) after 3, 6 and 24 h. The date was statistically analyzed with SPSS13.0 software package. RESULTS: A significant increase of the expression of gingival fibroblasts to inflammatory factors (IL-6, IL-8) after IL-1β was noted; PCR results of the production of inflammatory factors in gingival fibroblasts with the intervention of IL-1β showed that the production of inflammatory factors (IL-6, IL-8) on titanium and titanium alloy were less than that on the titanium nitride. CONCLUSIONS: IL-1β can stimulate high expression of inflammatory factors; The production of IL-6 and IL-8 on titanium and titanium alloy are less, suggesting that implant made of titanium and titanium alloy can reduce the incidence of peri-implant inflammation, and improve the successful rate of implantation.

Key words: Gingival fibroblasts, IL-1β, IL-6, IL-8

中图分类号:

R783.1

孙志新,张云涛. IL-1β对人牙龈成纤维细胞在不同钛板表面表达炎性因子的影响[J]. 上海口腔医学, 2015, 24(2): 151-156.

SUN Zhi-xin,ZHANG Yun-tao. Effect of IL-1β on inflammatory factors synthesis of human gingival fibroblasts attached to different titaniums[J]. Shanghai Journal of Stomatology, 2015, 24(2): 151-156.

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