3种树脂水门汀对人牙龈成纤维细胞的体外细胞毒性

doi:10.19439/j.sjos.2017.06.005

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (6): 594-598.doi: 10.19439/j.sjos.2017.06.005

3种树脂水门汀对人牙龈成纤维细胞的体外细胞毒性

张长源¹, 牛刚², 靳祎祎³, 于皓¹, 程辉^{1, 4}

1.福建医科大学附属口腔医院修复科,2.口腔颌面外科,福建福州 350002;
3.福建中医药大学中西医结合研究院,福建福州 350122;
4.福建医科大学口腔医学研究院,福建福州 350002

收稿日期:2017-06-26 修回日期:2017-08-23 出版日期:2017-12-20 发布日期:2018-01-09
通讯作者: 程辉,E-mail:ch.fj@163.com
作者简介:张长源（1981-）,男,硕士,E-mail:longsource@126.com
基金资助:
福建医科大学附属口腔医院院内科研课题

Cytotoxicity of 3 resin cements to human gingival fibroblasts

ZHANG Chang-yuan¹, NIU Gang², JIN Yi-yi³, YU Hao¹, CHENG Hui^{1, 4}

1.Department of Prosthodontic, 2.Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Fujian Medical University. Fuzhou 350002;
3.Integrated Chinese and Western medicines, Fujian University of Traditional Chinese Medicine. Fuzhou 350122;
4.Institute of Stomatology, Fujian Medical University. Fuzhou 350002, Fujian Province, China

Received:2017-06-26 Revised:2017-08-23 Online:2017-12-20 Published:2018-01-09

摘要/Abstract

摘要： 目的: 比较3种不同树脂水门汀对人牙龈成纤维细胞（human gingival fibroblasts, HGFs）的体外细胞毒性。方法: 采用CCK-8法检测3种树脂水门汀（Panavia F、RelyX^TM Unicem和Multilink Speed）48 h不同浓度（50%和100%）浸提液培养HGFs（ATCC,美国）24 h、72 h和120 h后的细胞相对增殖率（relative growth rate, RGR）,判断其细胞毒性。采用SPSS 20.0 软件包分析不同树脂水门汀对HGFs的RGR是否存在显著影响。结果: 3种树脂水门汀不同浓度（50%和100%）浸提液培养HGFs的RGR为60.25%~100.02%,细胞毒性分级为0~2级,各组之间差异无显著性（P>0.05）;固化过程中未涂布防氧化剂的Panavia F浸提液培养HGFs的RGR为10.67%~66.78%,细胞毒性分级为2~4级,与其他实验组相比,有显著差异（P<0.05）。结论: 3种树脂水门汀对体外培养的HGFs无细胞毒性,固化过程中未涂布防氧化剂的Panavia F对体外培养的HGFs有中度细胞毒性。

关键词: 树脂水门汀, 细胞毒性, 人牙龈成纤维细胞

Abstract: PURPOSE: To study the cytotoxicity of 3 resin cements to human gingival fibroblasts (HGFs). METHODS: Three resin cements (Panavia F, RelyX^TM Unicem and Multilink Speed) test samples were immersed and incubated in the culture medium for 48 h at 37℃. Cultured HGFs were exposed to two concentrations (50% and 100%) of material elutes for 24 h, 72 h and 120 h. The proliferation rate was evaluated using CCK-8 assay. The data were statistically analyzed by one-way analysis of variance using SPSS 20.0 software package. RESULTS: Relative growth rate(RGR) of all experimental groups ranged from 10.67% to 100.02%, the cytotoxicity grade of all groups was 0 to 4. There was no significant difference in the RGR among 3 resin cements, but the experimental group of Panavia F (uncovered with antioxidant) showed significantly lower RGR than other experimental groups. CONCLUSIONS: Panavia F, RelyX^TM Unicem and Multilink Speed exhibit no cytotoxicity to HGFs, Panavia F(uncovered with antioxidant) shows moderate cytotoxicity.

Key words: Resin cement, Cytotoxicity, Human gingival fibroblasts

中图分类号:

R783.1

张长源, 牛刚, 靳祎祎, 于皓, 程辉. 3种树脂水门汀对人牙龈成纤维细胞的体外细胞毒性[J]. 上海口腔医学, 2017, 26(6): 594-598.

ZHANG Chang-yuan, NIU Gang, JIN Yi-yi, YU Hao, CHENG Hui. Cytotoxicity of 3 resin cements to human gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2017, 26(6): 594-598.

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3种树脂水门汀对人牙龈成纤维细胞的体外细胞毒性

Cytotoxicity of 3 resin cements to human gingival fibroblasts

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