CD4+ T细胞在不同菌株诱导小鼠牙周炎中的表达

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (1): 20-25.

CD4⁺ T细胞在不同菌株诱导小鼠牙周炎中的表达

王琳源, 靳赢, 林晓萍

中国医科大学附属盛京医院口腔科,辽宁沈阳 110004

收稿日期:2013-05-07 修回日期:2013-06-15 出版日期:2014-02-20 发布日期:2014-10-21
通讯作者: 林晓萍,Tel:024-96615-61522,E-mail: xiaoping_ba@126.com
作者简介:王琳源(1980-),女,主治医师,博士研究生,Tel:024-96615-61522,E-mail: wanglinyuan2006@163.com
基金资助:
高等学校博士学科点专项科研基金(20112104110013); 辽宁省科学技术计划项目(2012225015)

The expression of CD4⁺ T cells in different bacteria strain-induced mice periodontitis

WANG Lin-yuan, JIN Ying, LIN Xiao-ping

Department of Stomatology, Shengjing Hospital of China Medical University. Shenyang 110004, Liaoning Province, China

Received:2013-05-07 Revised:2013-06-15 Online:2014-02-20 Published:2014-10-21
Supported by:
Higher School Specialized Research Fund for Doctoral Program(20112104110013); Science and Technology Plan of Liaoning Province(2012225015)

摘要/Abstract

摘要： 目的：比较2种牙周致病菌菌株诱导的小鼠牙周炎中CD4⁺T细胞的表达,探讨CD4⁺T细胞在牙周炎中的作用。方法：将7周龄C57BL/6小鼠12只随机分为3组,分别是①对照组;②牙龈卟啉单胞菌 (Porphyromonas gingivalis, P. gingivalis) W83 感染组;③P. gingivalis ATCC 33277感染组。分别于感染后第4周取材,进行骨吸收度评价和牙周组织病理学观察,采用流式细胞术检测牙龈及颈淋巴结中CD4⁺T细胞的表达,采用SPSS11.0软件包对数据进行统计学分析。结果：与感染对照组小鼠相比,P. gingivalis W83 感染组和P. gingivalis ATCC 33277感染组小鼠均出现明显的牙槽骨吸收。组织病理学检查显示,牙周附着丧失,破骨细胞性骨吸收,牙龈及颈淋巴结中CD4⁺T细胞明显增加,并且P. gingivalis W83 感染组小鼠的牙周破坏和CD4⁺T细胞的变化较P. gingivalis ATCC 33277感染组小鼠更为明显。结论：CD4⁺T细胞参与牙周炎的发病过程,并且与牙周组织破坏密切相关。

关键词: CD4+ T细胞, 牙周炎, 动物模型

Abstract: PURPOSE: To compare the expression of CD4⁺ T cells in mice periodontitis induced by two periodontal pathogen strains, and evaluate the role of CD4⁺ T cells in periodontitis. METHODS: Twelve C57BL/6 mice were divided into 3 groups at random including sham-infected, Porphyromonas gingivalis (P. gingivalis) W83-infected and P. gingivalis ATCC 33277-infected groups. All mice were sacrificed at the 4th week after the last infection. The analysis of alveolar bone resorption and the examination of histological staining were performed. The population of CD4⁺ T cells in the gingivae and the cervical lymph nodes (CLNs) were analyzed by flow cytometry. SPSS 11.0 software package was used for statistical analysis. RESULTS: The levels of alveolar bone resorption were significantly high in both P. gingivalis W83-infected and P. gingivalis ATCC 33277-infected mice compared to sham-infected mice. Histological staining showed that the loss of periodontal attachment and osteoclasts-mediated alveolar bone resorption were found in both P. gingivalis W83-infected and P. gingivalis ATCC 33277-infected mice. Moreover, the periodontal destruction was severer in P. gingivalis W83-infected mice than in P. gingivalis ATCC 33277-infected mice. Flow cytometry showed that the percentage of CD4⁺ T cells in the gingivae and CLNs in both P. gingivalis W83-infected and P. gingivalis ATCC 33277-infected mice were significantly higher than that in sham mice, and P. gingivalis W83-infected mice had a higher percentage of CD4⁺ T cells compared to P. gingivalis ATCC 33277-infected mice. CONCLUSIONS: The population of CD4⁺ T cells participates in the pathogenesis of periodontitis, and closely correlates with the periodontal destruction induced by periodontal pathogen infection.

Key words: CD4+ T cell, Periodontitis, Animal model

中图分类号:

R781.4

王琳源, 靳赢, 林晓萍. CD4⁺ T细胞在不同菌株诱导小鼠牙周炎中的表达[J]. 上海口腔医学, 2014, 23(1): 20-25.

WANG Lin-yuan, JIN Ying, LIN Xiao-ping. The expression of CD4⁺ T cells in different bacteria strain-induced mice periodontitis[J]. Shanghai Journal of Stomatology, 2014, 23(1): 20-25.

参考文献

[1] Ohlrich EJ, Cullinan MP, Seymour GJ. The immunopathogenesis of periodontal disease [J]. Aust Dent J, 2009, 54(Suppl 1): S2-10.
[2] Hern&#x0dbc0;&#x0dd61;ndez M, Dutzan N, García-Sesnich J, et al. Host-pathogen interactions in progressive chronic periodontitis [J]. J Dent Res, 2011, 90(10): 1164-1170.
[3] Kobayashi R, Kono T, Bolerjack BA, et al. Induction of IL-10-producing CD4 + T-cells in chronic periodontitis [J]. J Dent Res, 2011, 90(5): 653-658.
[4] 苏军, 刘鲁川, 毛永利, 等. SD大鼠牙周炎动物模型的建立 [J]. 牙体牙髓牙周病学杂志, 2006, 16(11): 612-615.
[5] 杜建东, 余占海, 杨倩, 等. 实验性牙周炎动物模型研究 [J]. 实用口腔医学杂志, 2007, 23(6): 801-804.
[6] 黄绮凌, 宋宁, 李东健, 等. 黄芩对小鼠实验性牙周炎血清IgG1和IgG2a水平影响的研究[J]. 中国病理生理杂志, 2011, 27(1): 171- 174.
[7] 谢敏, 黄世光, 宋宁, 等. Th1极化反应与牙周炎病理改变的实验研究 [J]. 实用口腔医学杂志, 2010, 269(4): 457-460.
[8] 李云鹏, 刘大力, 束蓉, 等. 牙龈卟啉单胞菌临床菌株的分离、鉴定及生长特性分析 [J]. 上海交通大学学报 (医学版), 2012, 32(1): 64-68.
[9] 刘静波, 潘亚萍, 许学斌, 等. 牙龈卟啉单胞菌 PG0839 基因突变株生物学特性和毒力的初步研究 [J]. 上海口腔医学, 2011, 20(5): 449-453.
[10] Lin X, Han X, Kawai T, et al. Antibody to receptor activator of NF-κB ligand ameliorates T cell-mediated periodontal bone resorption [J]. Infect Immun, 2011, 79(2): 911-917.
[11] 林晓萍, 韩晓哲, Toshihisa Kawai, 等. NF-κB受体激活蛋白配体抗体抑制T细胞介导的牙周骨吸收 [J]. 中华口腔医学杂志, 2011, 46(10): 621-624.
[12] 赵蕾, 吴亚菲, 杨禾, 等. 成人牙周健康状况与fimA基因型牙龈卟啉单胞菌的相关性 [J]. 华西口腔医学杂志, 2007, 25(3): 237-241.
[13] 潘春玲, 刘俊超, 潘亚萍, 等. 牙龈卟啉单胞菌对人牙周膜成纤维细胞MMP-1和TIMP-1 表达影响的研究 [J]. 中国实用口腔科杂志, 2012, 5(4): 210-213.
[14] Seymour GJ, Crouch MS, Powell RN, et al. The identification of lymphoid cell subpopulations in sections of human lymphoid tissue and gingivitis in children using monoclonal antibodies [J]. J Periodontal Res, 1982, 17(3): 247-256.
[15] Guyodo H, Meuric V, Le Pottier L, et al. Colocalization of Porphyromonas gingivalis with CD4 + T cells in periodontal disease[J]. FEMS Immunol Med Microbiol, 2012, 64(2): 175-183.
[16] Graves D. Cytokines that promote periodontal tissue destruction[J]. J Periodontol, 2008, 79(8 Suppl): 1585-1591.
[17] Gemmell E, Carter CL, Hart DN, et al. Antigen-presenting cells in human periodontal disease tissues [J]. Oral Microbiol Immunol, 2002, 17(6): 388-393.
[18] Zhao L, Zhou Y, Xu Y, et al. Effect of non-surgical periodontal therapy on the levels of Th17/Th1/Th2 cytokines and their transcription factors in Chinese chronic periodontitis patients [J]. J Clin Periodontol, 2011, 38(6): 509-516.