上海口腔医学

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (2): 156-161.doi: 10.19439/j.sjos.2022.02.008

• 论著 • 上一篇    下一篇

低能量微波照射对大鼠正畸牙移动及牙周组织改建的影响

刘维佳, 唐镇, 张琦, 姜莉萍   

  1. 南昌大学附属口腔医院,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西 南昌 330006
  • 收稿日期:2020-12-14 修回日期:2021-08-24 出版日期:2022-04-25 发布日期:2022-05-16
  • 通讯作者: 唐镇, E-mail: tangzhen307@aliyun.com
  • 作者简介:刘维佳（1992-）,女,硕士研究生,住院医师,E-mail: weijialiu1992@foxmail.com
  • 基金资助:
    江西省自然科学基金（20202BABL206068）

Effect of low-energy microwave irradiation on orthodontic tooth movement and periodontal tissue remodeling in rats

LIU Wei-jia, TANG Zhen, ZHANG Qi, JIANG Li-ping   

  1. Affiliated Stomatological Hospital of Nanchang University; the Key Laboratory of Oral Biomedicine of Jiangxi Province; Jiangxi Province Clinical Research Center for Oral Diseases.Nanchang 330006, Jiangxi Province,China
  • Received:2020-12-14 Revised:2021-08-24 Online:2022-04-25 Published:2022-05-16

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摘要： 目的： 探讨低能量微波照射对大鼠正畸牙移动及牙周组织改建的影响。方法： 将SD大鼠随机分为对照组和实验组。采用螺旋弹簧加力法,通过镍钛拉簧装置构建大鼠正畸模型。实验组每天1次,用微波治疗仪照射被移动的第一磨牙,每次30 min;对照组不施加任何干预措施。分别在造模当天、第7、14、21天处死大鼠,测量大鼠第一磨牙的移动距离。抗酒石酸酸性磷酸酶(TRAP)染色检测大鼠牙周组织中破骨细胞计数;免疫组织化学检测破骨细胞分化因子（ODF）的表达;实时荧光定量PCR（RT-PCR）检测白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）mRNA的表达。采用SPSS 20.0软件包对实验数据进行统计学分析。结果： 第7、14、21天时,与对照组相比,实验组大鼠第一磨牙移动距离、牙周组织中破骨细胞计数、ODF表达显著升高（P<0.05）,牙周组织中IL-6和TNF-α的mRNA表达显著下降（P<0.05）。结论： 低能量微波照射能明显加快正畸牙移动速度,抑制炎性基因表达,促进牙周组织改建。

关键词: 低能量微波照射, 正畸牙移动, 牙周组织改建, 炎性基因

Abstract: PURPOSE: To investigate the effect of low-energy microwave irradiation on the movement of orthodontic teeth and periodontal tissue reconstruction in rats. METHODS: SD rats were randomly divided into control group and experimental group. Helical spring force method was used to construct a rat orthodontic model through a nickel-titanium tension spring device. Rats in the experimental group were irradiated with a microwave treatment apparatus once a day to move the first molars for 30 minutes, while rats in the control group were not given any intervention. The rats were sacrificed on the day of modeling, 7 d, 14 d, and 21 d thereafter. The movement distance of the rat's first molars was measured. Tartrate-resistant acid phosphatase (TRAP) staining was used to detect osteoclast counts in rat periodontal tissues, immunohistochemistry was used to detect the expression of cell differentiation factor (osteoclast differentiation factor, ODF) in rat periodontal tissues; real-time fluorescence quantitative PCR(RT-PCR) was used to detect the mRNA expression of interleukin 6(IL-6) and tumor necrosis factor-α (TNF-α). SPSS 20.0 software package was used to analyze the experimental data. RESULTS: At 7, 14, 21 d, compared with the control group, the distance of the first molar movement, the count of osteoclasts in the periodontal tissue, and the expression of ODF in the experimental group were significantly increased (P<0.05), while the mRNA expression of IL-6 and TNF-α in periodontal tissues was significantly decreased (P<0.05). CONCLUSIONS: Low-energy microwave irradiation can significantly accelerate the movement of orthodontic teeth, inhibit the expression of inflammatory genes, and promote the reconstruction of periodontal tissue.

Key words: Low-energy microwave irradiation, Orthodontic tooth movement, Periodontal tissue reconstruction, Inflammatory genes

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