大鼠正畸牙移动牙周组织中叉头框蛋白O1和成骨特异转录因子RUNX2的表达

doi:10.19439/j.sjos.2018.03.002

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (3): 230-234.doi: 10.19439/j.sjos.2018.03.002

大鼠正畸牙移动牙周组织中叉头框蛋白O1和成骨特异转录因子RUNX2的表达

周璨，龙思岑，黄兰，戴红卫

重庆医科大学附属口腔医院，口腔疾病与生物医学重庆市重点实验室，重庆市高校市级口腔生物医学工程重点实验室，重庆 401147

收稿日期:2017-12-12 修回日期:2018-02-07 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: 戴红卫，E-mail: dai_tg@163.com
作者简介:周璨（1993-），女，硕士，E-mail: 367043560@qq.com
基金资助:
国家自然科学基金（81300914，81400541）；重庆高校创新团队建设计划资助项目（CXTDG201602006）；重庆市高校市级口腔生物医学工程重点实验室资助项目

Expression of Forkhead boxO1 and Runt-related transcription factor 2 in periodontal tissue during orthodontic movement of teeth in rats

ZHOU Can, LONG Si-cen, HUANG Lan, DAI Hong-wei.

Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education. Chongqing 401147, China

Received:2017-12-12 Revised:2018-02-07 Online:2018-07-20 Published:2018-07-20

摘要/Abstract

摘要： 目的： 检测大鼠正畸牙移动牙周组织中叉头框蛋白O1（forkhead boxO1， FoxO1）和Runt相关转录因子2 (runt-related transcription factor 2，Runx2)的表达变化，初步探讨FoxO1和Runx2在正畸牙移动牙周组织改建中的作用及相互关系。方法： 将40只8周龄雄性Sprague-Dawley（SD）大鼠随机分为5组，建立正畸牙移动模型，以上颌左侧加力的第一磨牙为实验侧，右侧不加力的第一磨牙为对照侧。分别加力1、3、5、7、14 d后处死大鼠，解剖分离出含有第一磨牙的牙槽骨制备标本，进行苏木精-伊红（H-E）染色和FoxO1、Runx2免疫组织化学染色，利用 Image Pro Plus图像分析系统对染色后的切片做定量分析，采用 SPSS 13.0 软件包对数据进行统计学处理。结果： FoxO1在牙周膜主要表达于成骨细胞及成牙骨质细胞中，Runx2主要表达于成骨细胞、成纤维细胞及成牙骨质细胞中。实验组加力后，大鼠牙周组织中FoxO1和Runx2的表达增强，在3~5 d内达到峰值，而后表达降低；14 d时与对照组相比无显著差异（P>0.05），其余组与对照组相比均有显著差异(P<0.05)。结论： FoxO1和Runx2参与了正畸牙移动中的牙周组织改建，且主要参与了成骨细胞和骨形成的过程。

关键词: [关键字] 叉头框蛋白O1, 成骨特异转录因子RUNX2, 正畸牙移动, 牙周组织改建

Abstract: PURPOSE: To investigate the expression of FoxO1 and Runx2 in periodontal tissue and their effect during orthodontic teeth movement(OTM) in rats. METHODS: Forty 8-week old male Sprague-Dawley (SD) rats were used to establish animal models of orthodontic teeth movement and divided into 5 groups randomly. The right side of jaws of each rat was set as experimental side, and the left side as control side. At 1, 3, 5, 7, 14 d after orthodontic treatment, the rats were sacrificed and the maxillary bone containing the first molar was dissected. H-E staining and immunohistochemical staining were used to detect the morphological changes, the expression of FoxO1 and Runx2 of the periodontal tissue at different points. Computer image analysis was used to evaluate the expression of FoxO1 and Runx2 in the periodontal tissues of the rats. The differences were analyzed by using SPSS19.0 software package. RESULTS: The expression of FoxO1 in periodontal ligament was mainly in osteoblasts and cementoblasts; and the expression of Runx2 was mainly in osteoblasts, fibroblasts and cementoblasts. In the experimental group,the expressions of FoxO1 and Runx2 in the periodontal tissues of rats increased significantly, reached the peak within 3-5 days, then decreased. There was no significant difference between the experimental group and the control group at 14th day (P>0.05), but significant difference was found between other group and control group (P<0.05). CONCLUSIONS: FoxO1 and Runx2 play a role in the reconstruction of periodontal tissue during orthodontic movement of teeth, and they are mainly involved in the process of osteoblast formation and bone formation.

Key words: Forkhead boxO1, Runt-related transcription factor2, Orthodontic tooth movement, Periodontal tissue remodeling

中图分类号:

R783.5

周璨，龙思岑，黄兰，戴红卫. 大鼠正畸牙移动牙周组织中叉头框蛋白O1和成骨特异转录因子RUNX2的表达[J]. 上海口腔医学, 2018, 27(3): 230-234.

ZHOU Can, LONG Si-cen, HUANG Lan, DAI Hong-wei.. Expression of Forkhead boxO1 and Runt-related transcription factor 2 in periodontal tissue during orthodontic movement of teeth in rats[J]. Shanghai Journal of Stomatology, 2018, 27(3): 230-234.

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大鼠正畸牙移动牙周组织中叉头框蛋白O1和成骨特异转录因子RUNX2的表达

Expression of Forkhead boxO1 and Runt-related transcription factor 2 in periodontal tissue during orthodontic movement of teeth in rats

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