Effect of deaminotyrosine on alveolar bone loss in periodontitis mice induced by a high-fat diet and its mechanism

doi:10.19439/j.sjos.2026.05.002

Abstract

Abstract: PURPOSE: To investigate the effects of the gut microbiota metabolite deaminotyrosine (DAT) on alveolar bone loss in mice with periodontitis (PD) induced by a high-fat diet and its potentional mechannism. METHODS: Thirty mice with similar growth status were randomly divided into a normal diet group (n=6) and a high-fat diet group (n=24). After 3 months of feeding, 6 mice were randomly selected from the high-fat diet group, and indicators such as body weight, fat content, and blood glucose were measured and compared with those of the normal diet group. The remaining 18 mice in the high-fat diet group were further divided into 3 subgroups: high-fat diet group (without PD model establishment), high-fat diet+PD group and high-fat diet+PD+DAT group (n=6 per group). PD model was established after 3 months of high-fat diet feeding, and the mice were treated after 4 weeks. The left maxilla was collected for Micro-CT scanning. The distance from the cemento-enamel junction (CEJ) to the top of the alveolar bone crest (ABC) (CEJ-ABC) and the bone volume fraction (BV/TV) were analyzed to evaluate the degree of alveolar bone loss. qRT-PCR was used to detect the expression levels of inflammatory factors (including IL-1β, IL-6, and TNF-α) in mouse serum. In in vitro experiments, the osteoblast cell line MC3T3-E1 was cultured and divided into the control group, 1 mmol/L DAT group, and 5 mmol/L DAT group. CCK-8 assay was used to detect cell proliferation in each group, while alkaline phosphatase (ALP) staining and Alizarin red (AR) staining were performed to observe the osteogenic differentiation of the cells. RESULTS: Compared with the high-fat diet+PD group, the high-fat diet+PD+DAT group showed a significant increase in CEJ-ABC distance and a significant decrease in BV/TV (P<0.05). The expression levels of IL-1β and TNF-α in the high-fat diet+PD+DAT group were significantly lower than those in the high-fat diet+PD group (P<0.05). For the MC3T3-E1 cell line, compared with the control group and the 1 mmol/L DAT group, the 5 mmol/L DAT group exhibited a significant inhibition of cell proliferation and osteogenic differentiation abilities. CONCLUSIONS: DAT can inhibit the proliferative activity and osteogenic differentiation capacity of osteoblasts, thereby leading to alveolar bone loss.

Key words: Deaminotyrosine, Osteoblasts, Bone loss, Periodontitis, High-fat diet, Osteogenic differentiation

CLC Number:

R781.4+2

Heng Xiao, Sun Jin, Hong Jialin, Wang Qiujin, Yang Yihan, Wang Hong, Liu Yan. Effect of deaminotyrosine on alveolar bone loss in periodontitis mice induced by a high-fat diet and its mechanism[J]. Shanghai Journal of Stomatology, 2026, 35(5): 456-463.

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