Mechanism of LncRNA H19 regulation of bone-directed induced differentiation of periodontal stem cells through NRF-2/HO-1 signaling pathway in inflammatory microenvironment

doi:10.19439/j.sjos.2025.06.003

Abstract

Abstract: PURPOSE: To investigate the effect and underlying mechanism of long non-coding RNA H19 on the osteogenic differentiation of periodontal ligament stem cells(PDLSCs) under inflammatory conditions. METHODS: PDLSCs were subjected to osteogenic and adipogenic induction. Alizarin Red staining and Oil Red O staining were used to observe osteogenic and adipogenic differentiation, respectively. The expression levels of osteogenesis-related genes RUNX-2, ALP, and BSP, as well as LncRNA H19, were measured before and after osteogenic induction. Cells were also cultured in an inflammatory environment to assess the expression of RUNX-2, ALP, BSP, LncRNA H19 and NRF-2/HO-1. Furthermore, LncRNA H19 was upregulated via lentiviral transfection, and subsequent changes in the expression of osteogenic genes (RUNX-2, ALP, BSP) and NRF-2/HO-1 were analyzed. RESULTS: PDLSCs had stem cell properties and could be induced to undergo osteogenic and adipogenic differentiation. During osteogenic differentiation, the expression of osteogenic genes RUNX-2, ALP, BSP and LncRNA H19 increased. Under inflammatory conditions, the expression of RUNX-2, ALP, BSP and LncRNA H19 was downregulated, along with a decrease in NRF-2/HO-1 expression. Upregulation of LncRNA H19 resulted in increased expression of NRF-2/HO-1 as well as osteogenic genes RUNX-2, ALP and BSP. CONCLUSIONS: The inflammatory microenvironment may suppress osteogenic differentiation of PDLSCs by reducing LncRNA H19 expression, which in turn inhibits the NRF-2/HO-1 signaling pathway.

Key words: LncRNAH19, NRF-2/HO-1 signaling pathway, Periodontal stem cells, Bone-oriented differentiation

CLC Number:

R781.4
Q254

Qian Yi, Xu Xiaobo, Wu Yan, Gong Wenting, Lu Jun, Liu Shouhong. Mechanism of LncRNA H19 regulation of bone-directed induced differentiation of periodontal stem cells through NRF-2/HO-1 signaling pathway in inflammatory microenvironment[J]. Shanghai Journal of Stomatology, 2025, 34(6): 577-582.

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