Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse

doi:10.19439/j.sjos.2024.02.001

Abstract

Abstract: PURPOSE: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse. METHODS: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package. RESULTS: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group. CONCLUSIONS: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.

Key words: Fluorosis, Condylar cartilage, Autophagy, MMP-13, LC3

CLC Number:

R780

YU Ying, WENG Qing-qing, LUO Yin-yue, YAO Shu-ran, YI Fang-yu, ZHANG Ying. Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse[J]. Shanghai Journal of Stomatology, 2024, 33(2): 113-116.

References

[1] 张素洁, 张忠诚, 徐祗云. 微量元素氟与人体健康[J]. 微量元素与健康研究, 2007, 24(2): 69-70.
[2] 胡德渝. 口腔预防医学[M]. 北京: 人民卫生出版社, 2012: 97-98.
[3] Nishida T, Kubota S, Aoyama E, et al.Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus[J]. Osteoarthritis Cartilage, 2013, 21(5): 700-709.
[4] Zhao Y, Hao J, Wang J, et al.Effect of choline on the composition and degradation enzyme of extracellular matrix of mice chondrocytes exposed to fluoride[J]. Biol Trace Elem Res, 2017, 175(2): 414-420.
[5] 郑冬冬. ClC-7参与氟斑牙和氟骨症发生的实验动物研究[D]. 西安: 第四军医大学, 2013.
[6] Chen S, He LH, Zhao L, et al.Effects of articular disc or condylar cartilage resection on mandibular growth in young rats[J]. Arch Oral Biol, 2019, 97: 67-71.
[7] 安康, 张元宇, 魏亚男, 等. DON毒素与氟对大鼠骨及关节软骨的损伤作用[J]. 中国地方病防治杂志, 2016, 31(11): 1201-1204.
[8] 李辉, 黄林剑, 谢千阳, 等. 体外培养的兔下颌骨髁突对机械压力的反应和变化[J]. 中国口腔颌面外科杂志, 2022, 20(4): 340-346.
[9] 许鹏, 郭雄, 姚建锋, 等. 过量氟对实验大鼠软骨细胞分化及Ⅱ和Ⅹ型胶原表型表达的影响[J].中华风湿病学杂志, 2002, 6(4): 231-234.
[10] Hu Q, Ecker M.Overview of MMP-13 as a promising target for the treatment of osteoarthritis[J]. Int J Mol Sci, 2021, 22(4): 1742-1762.
[11] 马瑞. 氟通过PHD2/HIF1α信号通路抑制SW1353软骨细胞糖酵解[D]. 沈阳:中国医科大学, 2021.
[12] 陈荣. 自噬相关因子Beclin1、LC3和p62在慢性氟中毒大鼠软骨中的表达意[D]. 贵阳: 贵州医科大学, 2017.
[13] Li JX, Yan Q, Liu N, et al.The prognostic value of autophagy-related markers Bclin-1 and LC-3 in colorectal cancers: a systematic review and meta-analysis[J]. Evid Based Complement Alternat Med, 2020: 8475840.
[14] 隋馨莹, 徐平, 段昌柱,等. p62蛋白的分子功能及其在疾病中的研究进展[J]. 生物工程学报, 2023, 39(4): 1374-1389.
[15] 翁清清, 易芳羽, 郁莹, 等. 自噬在氟牙症大鼠成釉细胞中的保护作用[J]. 口腔医学, 2020, 40(11): 982-986.