Different ways to remove necrotic periodontal ligament on delayed tooth replantation: scanning electron microscopy and histomorphometric analysis

Abstract

Abstract: PURPOSE: To seek the most effective technique for removal of root-adhered periodontal ligament. METHODS: Thirty-five roots of healthy right mandibular first premolars extracted for orthodontic purposes were selected. After extraction, the teeth were kept dry at room temperature for at least 1 h, and then divided into five groups as follows: Group 1(control)-the root-adhered periodontal ligament was preserved; Group 2 (mechanical removal)-periodontal ligament remnants were removed by scraping root surface with a scalpel blade; Group 3 (chemical removal 1)-removal of the periodontal ligament by immersing the root in 1% sodium hypochlorite solution for 5 minutes; Group 4 (chemical removal 2)-removal of the periodontal ligament by immersing the root in 2% sodium hypochlorite solution for 5 minutes; Group 5 (chemical removal 3)-removal of the periodontal ligament by immersing the root in 1% sodium hypochlorite solution for 10 minutes. The specimens were analyzed histomorphometrically and examined under scanning electron microscopy. Both periodontal ligament remnants and preservation or removal of the cementum layer concomitantly with these procedures were assessed. The data were analyzed using one-way ANOVA and Tukey's test with SAS 8.02 software package. RESULTS: In regard to periodontal ligament remnants, group 1 presented the largest areas(P<0.05), group 2 showed smaller area than group 1 (P<0.05), but much larger than group 3-5 (P<0.05); The three chemical removal groups had no significant difference. As for preserved cementum layer, group 2 lost most areas, which showed the worst result(P<0.05). The other 4 groups had no significant difference. CONCLUSIONS: Scraping root surface with a scalpel blade could not wipe off the root-adhered periodontal ligament, and might damage the cementum layer. Sodium hypochlorite solution could clean up the periodontal ligament remnants and preserve cementum layer, which might be an ideal choice.

Key words: Avulsion, Delayed tooth replantation, Periodontal ligament, Cementum, Sodium hypochlorite solution

CLC Number:

R788.4

CHEN Hui, ZHANG Yang, WANG Jun. Different ways to remove necrotic periodontal ligament on delayed tooth replantation: scanning electron microscopy and histomorphometric analysis[J]. Shanghai Journal of Stomatology, 2016, 25(3): 296-300.

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