Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor

Abstract

Abstract: PURPOSE: To investigate the effect of different concentrations of fluoride on the expression of endoplasmic reticulum chaperone, and to explore the mechanism of dental fluorosis in rat. METHODS: Thirty Wistar rats were randomly divided into 3 groups. Immunohistochemistry was used to detect the expression of CRT, GRP78, XBP-1 and caspase-12 in rat incisors. Metamorph microscope images analysis system and SPSS 13.0 software package was used to analyze the data. RESULTS: Typical features of dental fluorosis were found in the fluoride group. Results of immunohistochemistry showed that CRT (F=238.6, P<0.05), GRP78 (F=27.42, P<0.05), XBP-1 (F=139.7, P<0.05) and caspase-12 (F=43.91, P<0.05) were significantly different among the 3 groups. CONCLUSIONS: Excessive fluoride can increase the secretion of CRT, GRP78, XBP-1 and caspase-12 suggest the ameloblasts and in status of endoplasmic reticulum stress and caspase-12 plays an important role during ameloblast apoptosis.

Key words: Fluoride, Ameloblast, Endoplasmic reticulum stress

CLC Number:

R780.1

ZHANG Kai-qiang, ZHANG Ying, LIU Lu, GU He-feng, MA Lin. Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor[J]. Shanghai Journal of Stomatology, 2013, 22(5): 481-486.

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