槐角提取物对雌激素缺乏性骨质疏松小鼠牙槽骨骨量的影响

doi:10.19439/j.sjos.2024.03.007

上海口腔医学 ›› 2024, Vol. 33 ›› Issue (3): 255-259.doi: 10.19439/j.sjos.2024.03.007

槐角提取物对雌激素缺乏性骨质疏松小鼠牙槽骨骨量的影响

林惠^1,*, 高云飞^2,*, 卫超¹, 王梦蕾¹, 马旭辉³

1.上海交通大学医学院附属儿童医院口腔科,上海 200062;
2.上海市第八人民医院口腔科,上海 200030;
3.上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海交通大学口腔医学院, 国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室, 上海市口腔医学研究所,上海 200011

收稿日期:2023-12-01 修回日期:2024-03-22 出版日期:2024-06-25 发布日期:2024-07-11
通讯作者: 马旭辉,E-mail: dr_mxh@126.com
作者简介:林惠（1976-）,女,本科, E-mail: linhui@shchildren.com.cn;高云飞（1988-）,女,硕士,E-mail: 50791266@qq.com。^*共同第一作者

Effects of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice

LIN Hui¹, GAO Yun-fei², WEI Chao¹, WANG Meng-lei¹, MA Xu-hui³

1. Department of Dentistry, Children's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. Shanghai 200062;
2. Department of Dentistry, Shanghai Eighth People's Hospital. Shanghai 200030;
3. Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center of Oral Disease; Shanghai Key laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2023-12-01 Revised:2024-03-22 Online:2024-06-25 Published:2024-07-11

摘要/Abstract

摘要： 目的：探讨槐角提取物对雌激素缺乏性骨质疏松小鼠牙槽骨骨量的影响。方法：6周龄雌性未孕野生型C57BL/6J小鼠,随机分为假手术组、卵巢切除（ovariectomy, OVX）组和 OVX+槐角提取物组。OVX组和 OVX+槐角提取物组小鼠摘除双侧卵巢,按照每次150 mg/kg剂量槐角提取物对OVX+槐角提取物组小鼠进行灌胃处理,每周3次,连续给药4周,其余2组小鼠同期给予等体积生理盐水灌胃。每周测量体重3次,4周后以micro-CT检测小鼠牙槽骨骨微参数。采用GraphPad Prism 9软件对数据进行处理分析。结果：与假手术组相比,OVX组小鼠牙槽骨术后1个月骨小梁相关参数显著下降（P<0.05）。槐角提取物干预1个月后,雌激素缺乏小鼠牙槽骨骨密度（bone mineral density, BMD）、骨小梁数量（trabecular number, Tb.N）和骨小梁离散度（trabecular separation, Tb.Sp）明显恢复,与假手术组无显著差异（P>0.05）;但骨小梁面积体积比（bone volume fraction, BV/TV）和骨小梁宽度（trabecular thickness, Tb.Th）尚未完全恢复到假手术组水平（P<0.05）。结论：槐角提取物能有效增加雌激素缺乏而减小的牙槽骨骨量,可作为治疗绝经性牙槽骨骨质疏松症的潜在药物之一。

关键词: 槐角提取物, 卵巢切除, 牙槽骨, 骨质疏松

Abstract: PURPOSE: To investigate the effect of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice. METHODS: Six-week-old female non-pregnant wild-type C57BL/6J mice were randomly divided into sham operation group, ovariectomy(OVX) group and OVX+Sophora japonica extract group. Ovaries of the mice in the OVX group and the OVX+Sophora japonica extract group were removed, and the mice in the OVX+Sophora japonica extract group were treated by Sophora japonica extract at a dose of 150 mg/kg, three times a week for 4 weeks; while mice of the other two groups were given an equal volume of normal saline at the same time. Body weight was measured 3 times a week, and the micro-parameters of alveolar bone were detected by Micro-CT after 4 weeks. The data were analyzed by GraphPad Prism 9 software. RESULTS: Compared with the sham-operated group, the trabecular bone parameters of the alveolar bone in the OVX group were significantly decreased 1 month after operation (P<0.05). One month after intervention with Sophora japonica extract, alveolar bone mineral density (BMD), trabecular number (Tb.N) and trabecular separation(Tb.Sp) in OVX mice was significantly rescued, with no significant difference compared to the sham surgery group(P>0.05); but bone volume fraction(BV/TV) and trabecular thickness (Tb.Th) had not completely recovered to the levels of the sham-operated group(P<0.05). CONCLUSIONS: Sophora japonica extract can effectively increase the alveolar bone mass reduced by estrogen deficiency and may be used as one of the potential drugs for the treatment of menopausal alveolar bone osteoporosis.

Key words: Sophora japonica extract, Ovariectomy, Alveolar bone, Osteoporosis

中图分类号:

R782.1

林惠, 高云飞, 卫超, 王梦蕾, 马旭辉. 槐角提取物对雌激素缺乏性骨质疏松小鼠牙槽骨骨量的影响[J]. 上海口腔医学, 2024, 33(3): 255-259.

LIN Hui, GAO Yun-fei, WEI Chao, WANG Meng-lei, MA Xu-hui. Effects of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice[J]. Shanghai Journal of Stomatology, 2024, 33(3): 255-259.

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槐角提取物对雌激素缺乏性骨质疏松小鼠牙槽骨骨量的影响

Effects of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice

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