负载纳米羟磷灰石的结冷胶修复大鼠下颌骨缺损的效果评价

doi:10.19439/j.sjos.2022.05.001

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (5): 449-453.doi: 10.19439/j.sjos.2022.05.001

负载纳米羟磷灰石的结冷胶修复大鼠下颌骨缺损的效果评价

许来俊^1,2, 袁鹤¹, 叶青¹, 李继遥¹

1.口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心, 四川大学华西口腔医院牙体牙髓病科,四川成都 610041;
2.中南大学湘雅口腔医学院（湘雅口腔医院）牙体牙髓病科,湖南长沙 410008

收稿日期:2021-05-06 修回日期:2021-07-12 出版日期:2022-10-25 发布日期:2022-11-01
通讯作者: 李继遥,E-mail: jiyaoliscu@163.com
作者简介:许来俊（1990-）,男,博士研究生,E-mail: 14211220120@fudan.com
基金资助:
国家自然科学基金（81701028）; 湖南省自然科学基金（2022JJ40733）; 湖南省卫生健康委科研计划项目（202208015033）

Repair of mandibular defects with hydrogel loaded with nano-hydroxyapatite in rats

XU Lai-jun^1,2, YUAN He¹, YE Qing¹, LI Ji-yao¹

1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases; Department of Endodontics, West China Hospital of Stomatology, Sichuan University. Chengdu 610041, Sichuan Province;
2. Department of Endodontics, Xiangya School of Stomatology & Xiangya Stomatological Hospital, Central South University. Changsha 410008, Hunan Province, China

Received:2021-05-06 Revised:2021-07-12 Online:2022-10-25 Published:2022-11-01

摘要/Abstract

摘要： 目的: 观察负载纳米羟磷灰石的结冷胶（GG/nHA）修复大鼠下颌骨缺损的效果。方法: 于16只SD大鼠下颌骨制备直径为5 mm的临界骨缺损,随机分为2组,实验组骨缺损区注入GG/nHA,对照组骨缺损区填充可吸收性明胶海绵。术后4周、8周处死大鼠,以Micro-CT定量分析骨组织愈合情况。苏木精-伊红（H-E）染色和马松（Masson）染色定性评估骨组织修复情况。采用GraphPad Prism 8.0软件包对数据进行统计学分析。结果: 制备的GG/nHA具有良好的可注射性,可经注射器推出至骨缺损区。术后4周和8周,实验组缺损区的骨生成量及骨体积分数（BV/TV）均显著高于对照组（P<0.05）。H-E染色和Masson染色均见实验组较对照组有更多新骨形成。结论: GG/nHA可以注射形式注入下颌骨缺损区,促进其愈合,有望成为微创修复口腔颌面部骨缺损的新型材料。

关键词: 颌骨缺损, 水凝胶, 结冷胶, 纳米羟磷灰石, 骨再生

Abstract: PURPOSE: To observe the effect of gellan gum loaded with nano-hydroxyapatite (GG/nHA) on repairing mandibular defect in rats. METHODS: Critical bone defects with 5 mm in diameter on the mandible of 16 SD rats were created and randomly divided into two groups. The bone defects in the experimental group were injected with GG/nHA and the control group were filled with absorbable gelatin sponge. The rats were sacrificed at 4 and 8 weeks after operation. The bone tissue healing was evaluated by Micro-CT. Bone tissue repairing effect was evaluated by hematoxylin-eosin (H-E) staining and Masson staining. GraphPad Prism 8.0 software package was used for statistical analysis. RESULTS: The prepared GG/nHA had a good injectability and could be delivered to the bone defect area with a syringe. Four and 8 weeks after operation, the newly formed bone and bone volume fraction (BV/TV) in the experimental group were higher than those in the control group, and the difference was statistically significant (P<0.05). Larger number of new bone were observed in the experimental group than the control group by H-E staining and Masson staining. CONCLUSIONS: GG/nHA can be injected into the mandibular defect area to promote its healing, and it is expected to be used as a novel bio-material for minimally invasive repair of oral and maxillofacial bone defects.

Key words: Mandibular defects, Hydrogel, Gellan gum, Nano-hydroxyapatite, Bone regeneration

中图分类号:

R783.1

许来俊, 袁鹤, 叶青, 李继遥. 负载纳米羟磷灰石的结冷胶修复大鼠下颌骨缺损的效果评价[J]. 上海口腔医学, 2022, 31(5): 449-453.

XU Lai-jun, YUAN He, YE Qing, LI Ji-yao. Repair of mandibular defects with hydrogel loaded with nano-hydroxyapatite in rats[J]. Shanghai Journal of Stomatology, 2022, 31(5): 449-453.

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负载纳米羟磷灰石的结冷胶修复大鼠下颌骨缺损的效果评价

Repair of mandibular defects with hydrogel loaded with nano-hydroxyapatite in rats

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