miR-99a对口腔鳞癌增殖能力的影响

doi:10.19439/j.sjos.2021.01.009

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (1): 44-49.doi: 10.19439/j.sjos.2021.01.009

miR-99a对口腔鳞癌增殖能力的影响

王科, 彭国光, 谭玉莲, 何善志, 罗翠芬

广州中医药大学附属佛山市中医院口腔医疗中心,广东佛山 528000

收稿日期:2019-03-11 修回日期:2019-11-12 出版日期:2021-02-25 发布日期:2021-04-02
通讯作者: 彭国光,E-mail: guoguang828@yahoo.com.cn
作者简介:王科（1982-）,男,副主任医师,硕士研究生,E-mail: keke820403@163.com
基金资助:
佛山市医学重点专科培育项目（Fspy3-2015018）

MiR-99a inhibits proliferation of oral squamous cell carcinoma by targeting mTOR pathway

WANG Ke, PENG Guo-guang, TAN Yu-lian, HE Shan-zhi, LUO Cui-fen

Stomatological Medical Center of Foshan Traditional Chinese Medicine, Hospital Affiliated to Guangzhou University of Chinese Medicine. Foshan 528000, Guangdong Province, China

Received:2019-03-11 Revised:2019-11-12 Online:2021-02-25 Published:2021-04-02

摘要/Abstract

摘要： 目的:探讨miR-99a在口腔鳞状细胞中的表达及对口腔鳞癌细胞生物学行为的影响。方法:检索GEO 数据库中与口腔鳞癌相关的miRNA芯片进行二次分析。检测63 例口腔鳞癌组织和口腔鳞癌细胞株内miR-99a的表达,分析其与临床病理指标及患者预后之间的关系。上调miR-99a的表达,检测其对口腔鳞癌细胞生长和克隆形成的影响,并通过Targetscan等软件预测其靶基因。采用SPSS 19.0软件包对数据进行统计学分析。结果:GSE103931芯片显示,miR-99a在口腔鳞癌中下调最显著,且miR-99a也在口腔鳞癌细胞株内显著低表达。 miR-99a的表达与患者T分期、病理分级和预后密切相关。上调miR-99a的表达可抑制口腔鳞癌细胞增殖;而抑制miR-99a的表达,则促进口腔鳞癌细胞增殖。通过Targetscan等软件发现,mTOR是miR-99a的靶基因,且与miR-99a的表达呈负相关。结论:miR-99a在口腔鳞癌组织和细胞系中低表达。上调 miR-99a能抑制口腔鳞癌细胞株SCC25和SCC9的增殖能力, 其机制是通过调控mTOR的表达而实现。

关键词: miR-99a, 口腔鳞状细胞癌, mTOR, 细胞增殖

Abstract: PURPOSE: To investigate miR-99a expression and its effect on proliferation of oral squamous cell carcinoma (OSCC). METHODS: miRNA microarrays associated with OSCC were identified in GEO database. The expression levels of miR-99a were detected in 63 OSCC tissues and adjacent normal tissues and cell lines. The relationship between clinicopathological parameters and miR-99a expression was analyzed by using ANOVA analysis. The ability of cell growth and clone formation were examined in SCC9 and SCC25 cells transfected with miR-99a mimics. The target genes of miR-99a were predicted by Targetscan software. There resulting data were analyzed using SPSS 19.0 software package.RESULTS: The differently expressed miRNAs were analyzed based on GSE103931 microarray. miR-99a was significantly downregulated in OSCC tissues and cell lines. miR-99a expression was significantly associated with T stage, pathological grading and patients’ prognosis. miR-99a overexpression inhibited OSCC cell proliferation and clone formation, while miR-99a inhibition contributed to decreased proliferation and clone formation ability. In addition, miR-99a combined with mTOR gene’s 3’UTR was negatively correlated with mTOR expression in OSCC tissues. CONCLUSIONS: miR-99a functions as a tumor suppressor in OSCC and inhibits OSCC cell proliferation by targeting mTOR.

Key words: miR-99a, Oral squamous cell carcinoma, mTOR, Cell proliferation

中图分类号:

R739.8

王科, 彭国光, 谭玉莲, 何善志, 罗翠芬. miR-99a对口腔鳞癌增殖能力的影响[J]. 上海口腔医学, 2021, 30(1): 44-49.

WANG Ke, PENG Guo-guang, TAN Yu-lian, HE Shan-zhi, LUO Cui-fen. MiR-99a inhibits proliferation of oral squamous cell carcinoma by targeting mTOR pathway[J]. Shanghai Journal of Stomatology, 2021, 30(1): 44-49.

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miR-99a对口腔鳞癌增殖能力的影响

MiR-99a inhibits proliferation of oral squamous cell carcinoma by targeting mTOR pathway

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