茶黄素对变异链球菌浮游细菌和生物膜的体外抑制作用

doi:10.19439/j.sjos.2021.01.007

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (1): 33-37.doi: 10.19439/j.sjos.2021.01.007

茶黄素对变异链球菌浮游细菌和生物膜的体外抑制作用

郑沛¹, 张羽¹, 汪飒²

1.浙江中医药大学·口腔医学院,浙江杭州 310053;
2.浙江大学医学院附属口腔医院牙体牙髓病科,浙江杭州 310006

收稿日期:2020-01-14 修回日期:2020-02-24 出版日期:2021-02-25 发布日期:2021-04-02
通讯作者: 郑沛,E-mail：20161040@zcmu.edu.cn
作者简介:郑沛（1975-）,男,硕士研究生,讲师
基金资助:
浙江省2016年度高等教育课堂教学改革项目(kg20160187)

Inhibitory effects of theaflavin on planktonic and sessile bacterial cells of Streptococcus mutans in vitro

ZHENG Pei¹, ZHANG Yu¹, WANG Sa²

1. School of Stomatology, Zhejiang Chinese Medical University. Hangzhou 310053;
2. Department of Cariology and Endodontology, Affiliated Hospital of Stomatology, Medical College, Zhejiang University. Hangzhou 310006, Zhejiang Province, China

Received:2020-01-14 Revised:2020-02-24 Online:2021-02-25 Published:2021-04-02

摘要/Abstract

摘要： 目的:探讨茶黄素（theaflavin,TF）对变异链球菌浮游细菌和生物膜的抑制作用。方法:分别测定TF溶液、表没食子儿茶素没食子酸酯（EGCG）溶液、氯己定（CHX）溶液和乙醇对照溶液对变异链球菌UA159的最低抑菌浓度（minimum inhibitory concentration, MIC）和最低杀菌浓度（minimum bactericidal concentration, MBC）。体外构建变异链球菌UA159的生物膜,测定实验试剂对细菌增殖活性的影响及杀菌作用。采用SPSS 22.0软件包对数据进行统计学分析。结果:TF对变异链球菌UA159的MIC为500 μg/mL,MBC 为1 mg/mL。TF溶液浓度在2×MIC以上时,变异链球菌生物膜的代谢活性显著降低（P<0.05）,对生物膜也表现出较强的杀菌作用。结论:TF对已形成的变异链球菌UA159的生物膜具有显著的抑制活性以及杀菌作用,为TF的进一步临床应用提供了研究基础。

关键词: 变异链球菌, 茶黄素, 生物膜, 表没食子儿茶素没食子酸酯

Abstract: PURPOSE: Inhibitory effects of theaflavin(TF) on planktonic and sessile bacterial cells of Streptococcus mutans were investigated in the present study. METHODS: MIC and MBC of TF/EGCG/CHX on S. mutans UA159 were determined. Furthermore, biofilms were formed and treated with agents, then proliferative activity and viability of bacterial cells were detected. SPSS 22.0 software package was used for statistical analysis. RESULTS: TF was found to inhibit planktonic cells of S. mutans UA159 with MIC of 500 μg/mL and MBC of 1 mg/mL. Moreover, bacterial cells of S. mutans UA159 in the biofilms treated with TF at concentrations higher than 2 × MIC had lower proliferative activity and viability than those in control-treated biofilms. CONCLUSIONS: TF efficiently suppressed proliferative activity and viability of S. mutans biofilms, which provided theoretical basis for clinic applications of TF.

Key words: Streptococcus mutans, Theaflavin, Biofilm, Epigallocatechin gallate

中图分类号:

R781.3

郑沛, 张羽, 汪飒. 茶黄素对变异链球菌浮游细菌和生物膜的体外抑制作用[J]. 上海口腔医学, 2021, 30(1): 33-37.

ZHENG Pei, ZHANG Yu, WANG Sa. Inhibitory effects of theaflavin on planktonic and sessile bacterial cells of Streptococcus mutans in vitro [J]. Shanghai Journal of Stomatology, 2021, 30(1): 33-37.

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茶黄素对变异链球菌浮游细菌和生物膜的体外抑制作用

Inhibitory effects of theaflavin on planktonic and sessile bacterial cells of Streptococcus mutans in vitro

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