NF-κB信号转导通路与复发性口腔溃疡的相关性分析

doi:10.19439/j.sjos.2018.05.014

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (5): 513-517.doi: 10.19439/j.sjos.2018.05.014

NF-κB信号转导通路与复发性口腔溃疡的相关性分析

张敬¹, 令狐瑞琦², 沙晶晶³, 胡欣培⁴, 李丹¹, 李梦源¹

1.宁夏医科大学总医院口腔医院牙体牙髓病科,宁夏银川 750004;
2.西安小白兔集团吉祥分院,陕西西安 710077;
3.島根大学医学部,日本出云 6938501;
4.宁夏医科大学研究生院,宁夏银川 750004

收稿日期:2017-07-28 出版日期:2018-10-25 发布日期:2018-11-05
通讯作者: 张敬,E-mail: zhj20045@163.com
作者简介:张敬(1969-),女,硕士,主任医师
基金资助:
宁夏医科大学校级科研重点项目（XZ2016014）

Relationship between nuclear factor-κB signaling pathway and susceptibility of recurrent aphthous ulcer

ZHANG Jing¹, LINGHU Rui-qi², SHA Jing-jing³, HU Xin-pei⁴, LI Dan¹, LI Meng-yuan¹

1.Department of Endodontics, Stomatological Hospital of Ningxia Medical University General Hospital. Yinchuan 750004, Ningxia Hui Autonomous Region, China;
2.Rabbit Dental Group, Jixiang Branch of Xi'an City. Xi'an 710077, Shaanxi Province, China;
3.Shimane University Medical Department. Izumo 6938501, Japan;
4.Graduate School of Ningxia Medical University. Yinchuan 750004, Ningxia Hui Autonomous Regions, China

Received:2017-07-28 Online:2018-10-25 Published:2018-11-05

摘要/Abstract

摘要： 目的: 研究NF-κB信号转导通路与复发性口腔溃疡（RAU）易感性的相关性。方法: 采用ELISA方法检测124例RAU患者和133例健康对照者中NFκBp50、NFκBp65、IκBα、IKK的血清含量,则用限制性片段长度多态性-聚合酶链式反应（RFLP-PCR）进行NFκB-94 ins/del ATTG位点的基因多态性分析,用比值比（OR）和95%可信区间（95%CI）估计相对危险度,采用SPSS 20.0软件包对数据进行统计学分析。结果: RAU组患者血清NFκBp50、NFκBp65、IKK 水平均显著低于正常对照组（P<0.05）,RAU组患者血清IκBα水平均显著高于正常对照组（P<0.05）。NFκB-94 ins/del ATTG位点在基因型频率与等位基因频率的分布上,病例组与正常对照组之间均有显著差异（P<0.05）。NFκB-94 ins/del ATTG位点基因型ID（OR=3.073,95%CI=1.557-6.067）与DD（OR=4.851,95%CI=2.264-10.393）为高风险基因型,D等位基因为高风险等位基因（OR=2.079,95%CI=1.462-2.957）。结论: NF-κB信号转导通路与复发性口腔溃疡有一定关系。NFκB-94 ins/del ATTG位点是RAU患者的易感基因位点。NFκB-94 ins/del ATTG位点携带D等位基因,患RAU的风险性是携带I等位基因的2.079倍。

关键词: NFκB-94 ins/del ATTG, 复发性口腔溃疡, 信号转导通路, 基因多态性

Abstract: PURPOSE: To explore the possible relationship between recurrent aphthous ulcer (RAU) and nuclear factor-κB signaling pathway. METHODS: A total of 124 RAU patients were recruited for this study. The control group consisted of 133 healthy subjects. Serum NFκBp50, NFκBp65, IκBα and IKK concentration were detected by ELISA.NFκB-94 ins/del ATTG sites were detected by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Relative risk ratios were estimated by odds ratios (OR) and 95% confidence interval (95%CI). Statistical analysis was performed using SPSS 20.0 software package. RESULTS: Serum NFκBp50, NFκBp65 and IKK levels in RAU patients were significantly lower than those of the controls (P<0.05). Serum IκBα level in RAU patients was significantly higher than those of the controls (P<0.05). Significant differences were found in the genotype frequencies or allele frequencies of NFκB-94 ins/del ATTG sites between RAU patients and controls (P<0.05). ID genotype（OR=3.073,95%CI=1.557-6.067）, DD genotype (OR=4.851,95%CI=2.264-10.393), and D allele （OR=2.079,95%CI=1.462-2.957） at NFκB-94 ins/del ATTG site exhibited high risks of RAU. CONCLUSIONS: NF kappa B signaling pathway is associated with RAU.NFκB-94 ins/del ATTG sites are associated with higher risk of RAU. NFκB-94 ins/del ATTG D allele may serve as genetic determinants for RAU.

Key words: Nuclear factor-κB -94 ins/del ATTG, Recurrent aphthous ulcer, Signaling pathway, Gene polymorphism

中图分类号:

R781.5

张敬, 令狐瑞琦, 沙晶晶, 胡欣培, 李丹, 李梦源. NF-κB信号转导通路与复发性口腔溃疡的相关性分析[J]. 上海口腔医学, 2018, 27(5): 513-517.

ZHANG Jing, LINGHU Rui-qi, SHA Jing-jing, HU Xin-pei, LI Dan, LI Meng-yuan. Relationship between nuclear factor-κB signaling pathway and susceptibility of recurrent aphthous ulcer[J]. Shanghai Journal of Stomatology, 2018, 27(5): 513-517.

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NF-κB信号转导通路与复发性口腔溃疡的相关性分析

Relationship between nuclear factor-κB signaling pathway and susceptibility of recurrent aphthous ulcer

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