IL-10多态性对牙周炎患者牙周微生态的影响

doi:10.19439/j.sjos.2020.05.017

摘要/Abstract

摘要： 目的：探讨IL-10多态性对牙周炎患者牙周微生态的影响。方法：选取2018年6月—2019年3月淄博市第一医院收治的牙周炎患者作为实验组（n=79）,按照牙周炎类型分为中度牙周炎（n=47）与重度牙周炎（n=32）。另选择同期来院体检的牙周健康人作为对照组（n=50）。采用实时荧光定量PCR技术检测所有研究对象龈下牙菌斑中细菌定植水平;抽取静脉血,通过PCR技术检测IL-10中的-1082、-819、-592位点上的基因型及等位基因频率。通过多因素分析,探讨IL-10不同基因型与龈下牙菌斑中细菌定植水平的关系。采用SPSS 22.0软件包对数据进行统计学分析。结果：中度牙周炎患者伴放线放线杆菌(Actinomycetes actinomycetes,A.a)定植量显著低于重度牙周炎患者（P<0.05）;中度牙周炎与重度牙周炎患者牙龈卟啉单胞菌（Porphyromonas gingivalis,P.g）及A.a定植量均显著高于对照组（P<0.05）。中度牙周炎患者中,-819位点的CC基因型比例显著低于对照组,TT基因型比例显著高于对照组（P<0.05）;-819位点CC、TC、TT基因型比例与重度牙周炎患者相比无显著差异（P>0.05）;-592位点的CC基因型比例显著低于对照组,AA基因型比例显著高于对照组（P<0.05）。中度牙周炎中,-592位点的AA基因型患者A.a定植量显著高于本组AC基因型患者（P<0.05）;-819位点的TT基因型患者A.a定植量显著高于本组TC基因型患者（P<0.05）。多因素分析显示,中度牙周炎中IL-10-592-AA基因型、-819-TT基因型与A.a定植水平密切相关（P<0.05）。结论：IL-10基因多态性是中度牙周炎患者龈下微生态环境的影响因素,其中,IL-10-592-AA基因型、-819-TT基因型与A.a定植水平相关性最明显。

关键词: IL-10基因多态性, 牙周炎, 牙周微生态

Abstract: PURPOSE: To investigate the effect of IL-10 gene polymorphism on periodontal microecology in patients with periodontitis. METHODS: Seventy-nine patients with periodontitis (experimental group) and 50 healthy individuals (control group) visiting Zibo First Hospital from June 2018 to March 2019 were enrolled. The experimental group was further divided into two groups according to the severity of periodontitis, namely moderate periodontitis (n=47) and severe periodontitis (n=32). Real-time quantitative PCR was used to detect the level of bacterial colonization in the subgingival plaque of all subjects. The genotype and allele frequencies at-1082,-819, and-592 loci in IL-10 were detected by PCR. Multivariate analysis was used to explore the relationship between IL-10 genotypes and bacterial colonization level in subgingival plaque. The data were processed by SPSS 2.0 software package. RESULTS: Actinomycetes actinomycetes (A.a) colonization in patients with moderate periodontitis was significantly lower than that of patients with severe periodontitis (P<0.05). The amount of Porphyromonas gingivalis(P.g) and A.a in patients with moderate periodontitis and severe periodontitis was significantly higher than that in the control group (P<0.05). The proportion of CC genotypes in the-819 locus of patients with moderate periodontitis was significantly lower than that of the control group, and the proportion of TT genotype was significantly higher than that of the control group(P<0.05). The proportion of CC, TC and TT genotypes showed no significant difference between patients with moderate periodontitis and those with severe periodontitis (P>0.05). The proportion of CC genotypes in the-592 locus of patients with moderate periodontitis was significantly lower than that of the control group, and the proportion of AA genotype was significantly higher than that of the control group (P<0.05). In moderate periodontitis, A.a colonization amount at-592 gene locus of AA genotype was significantly higher than that of AC genotype(P<0.05). In moderate periodontitis, A.a colonization amount at of-819 gene locus of TT genotype was significantly higher than that of TC genotype (P<0.05). Multivariate analysis showed that IL-10-592-AA genotype and-819-TT genotype were closely related to A.a colonization in moderate periodontitis (P<0.05). CONCLUSIONS: IL-10 gene polymorphism is the influencing factor of subgingival microecological environment in patients with moderate periodontitis, among which IL-10-592-AA genotype and-819-TT genotype have the most obvious correlation with A.a colonization level.

Key words: IL-10 gene polymorphism, Periodontitis, Periodontal microecology

中图分类号:

R781.4

陈晓华, 徐茂林. IL-10多态性对牙周炎患者牙周微生态的影响[J]. 上海口腔医学, 2020, 29(5): 533-538.

CHEN Xiao-hua, XU Mao-lin. Effect of IL-10 gene polymorphism on periodontal microecology in patients with periodontitis[J]. Shanghai Journal of Stomatology, 2020, 29(5): 533-538.

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