耐氟变形链球菌对树脂-牙本质粘接强度的影响

doi:10.19439/j.sjos.2018.02.004

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (3): 239-243.doi: 10.19439/j.sjos.2018.02.004

耐氟变形链球菌对树脂-牙本质粘接强度的影响

汪嘉，何智妍，冉淑君，朱来宽

上海交通大学医学院附属第九人民医院·口腔医学院牙体牙髓病科，国家口腔医学疾病临床研究中心，上海市口腔医学重点实验室，上海市口腔医学研究所，上海 200011

收稿日期:2017-09-07 修回日期:2017-11-16 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: 朱来宽，E-mail：zhulk1997@163.com
作者简介:汪嘉（1986-），女，博士，主治医师，E-mail：tangelwj@163.com
基金资助:
中华口腔医学会牙科粘接技术临床研究青年医师专项（CSA-B2015-02）

Effect of fluoride-resistant strains of Streptococcus mutans on the bonding strength of dental resin composite

WANG Jia, HE Zhi-yan, RAN Shu-jun, ZHU Lai-kuan.

Department of Endodontics and Operative Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center of Stomatology; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2017-09-07 Revised:2017-11-16 Online:2018-07-20 Published:2018-07-20

摘要/Abstract

摘要： 目的： 探讨耐氟变形链球菌对树脂牙本质粘接强度的影响。方法： 诱导培养耐氟变形链球菌UA159-FR及其亲代菌株UA159，选用复合树脂Z350和自酸蚀粘接剂Single bond Universal，制备牙本质-树脂复合体试件。将试件分为4组，分别浸入UA159-FR菌液、UA159菌液、BHI培养基、PBS中孵育14 d，每48 h换液1次，之后进行推出试验，检测试件粘接强度的变化。扫描电镜下观察4组粘接断面的情况。采用SPSS18.0软件包对数据进行统计学分析。结果： 在UA159-FR和UA159侵袭下，牙本质-复合树脂粘接强度显著降低，组间无显著差异。粘接界面多为混合破坏，扫描电镜下可见大量的树脂突及部分粘接面破坏。结论： 耐氟变形链球菌可破坏复合树脂粘接界面，引起粘接强度下降，破坏程度在短期内与亲代菌株无显著差异。

关键词: 牙本质-树脂复合体, 变形链球菌耐氟菌株, 变形链球菌, 粘接强度

Abstract: PURPOSE: The aim of this study was to determine the influence of fluoride-resistant strains of S. mutans on the bonding strength of dental resin composite. METHODS: Standardized specimens of resin composite (Z350), and self-etch adhesives (Single bond Universal, 3M) were incubated with S. mutans UA159, fluoride-resistant strains of S. mutans UA159 (UA159-FR) or uninoculated culture medium (Control) or PBS for up to 14 days. Resin-dentin bonded disks were subjected to push-out test. Fractured surface analysis of the specimens was performed by scanning electron microscopy(SEM). SPSS18.0 software package was used for statistical analysis. RESULTS: UA159-FR and UA159 were shown to decrease the bond strengths in levels comparable with those found in the control groups (P<0.05). SEM confirmed the increased degradation of all materials with UA159-FR and UA159, compared with control and PBS. CONCLUSIONS: The results suggest that resin-dentin interface can be compromised by oral bacteria that contribute to the progress of secondary caries. Fluoride-resistant strains of S. mutans and its parental strains both decrease bonding strengths in a short time, but no significant difference in the extent of damage.

Key words: Dental resin composite, Fluoride-resistant Streptococcus mutans, Streptococcus mutans, Bond strengths

中图分类号:

R781.3

汪嘉，何智妍，冉淑君，朱来宽. 耐氟变形链球菌对树脂-牙本质粘接强度的影响[J]. 上海口腔医学, 2018, 27(3): 239-243.

WANG Jia, HE Zhi-yan, RAN Shu-jun, ZHU Lai-kuan.. Effect of fluoride-resistant strains of Streptococcus mutans on the bonding strength of dental resin composite[J]. Shanghai Journal of Stomatology, 2018, 27(3): 239-243.

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耐氟变形链球菌对树脂-牙本质粘接强度的影响

Effect of fluoride-resistant strains of Streptococcus mutans on the bonding strength of dental resin composite

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