Survivin和hsa-miR-542-3p在口腔鳞状细胞癌中的表达和相关性分析

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (6): 720-724.

Survivin和hsa-miR-542-3p在口腔鳞状细胞癌中的表达和相关性分析

吴宁¹, 陆瑛², 梁继宗¹

1.上海市第七人民医院口腔科,上海 200137;
2.上海市杨浦区市东医院口腔科,上海 200438

收稿日期:2016-02-17 出版日期:2016-12-25 发布日期:2016-12-29
通讯作者: 梁继宗,E-mail: liangjizong1234@sina.com
作者简介:吴宁（1980-）,男,学士,主治医师,E-mail：13361951308@163.com

Expression and correlation of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma

WU Ning¹, LU Ying², LIANG Ji-zong¹

1.Department of Stomatology, Shanghai Seventh People's Hospital. Shanghai 200137;
2. Department of Stomatology, Shanghai Shidong Hospital. Shanghai 200438, China

Received:2016-02-17 Online:2016-12-25 Published:2016-12-29

摘要/Abstract

摘要： 目的探讨survivin和hsa-miR-542-3p在口腔鳞状细胞癌(OSCC)中的表达和相关性。方法检测30例OSCC肿瘤组织及癌旁组织中survivin和hsa-miR-542-3p的表达水平,在OSCC细胞系中验证hsa-miR-542-3p对survivin表达的调控作用;检测30例OSCC患者和30例正常成人血浆中hsa-miR-542-3p的表达水平。采用SPSS17.0 软件包对数据进行统计学处理。结果OSCC样本中survivin表达上升,而hsa-miR-542-3p表达下降,两者呈负相关。在SCC-15细胞系中,hsa-miR-542-3p对survivin的mRNA和蛋白水平起负调控作用。OSCC患者血浆中hsa-miR-542-3p比正常人显著下降,ROC 曲线分析显示其AUC=0.82。结论在OSCC中,hsa-miR-542-3p降低与survivin上升相关。血浆hsa-miR-542-3p是潜在的OSCC 诊断标志物。

关键词: 口腔鳞状细胞癌, microRNA, Survivin, 生物标志物

Abstract: PURPOSE: To detect the expression of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma (OSCC) and explore their correlation with clinicopathologic parameters. METHODS: Protein levels of survivin and hsa-miR-542-3p were measured in 30 OSCC patients and SCC-15 cell line. Plasma hsa-miR-542-3p level was analyzed in 30 OSCC patients and 30 normal controls. Statistical analysis was performed with SPSS 17.0 software package. RESULTS: In OSCC samples, survivin was increased while hsa-miR-542-3p was decreased. There was a negative correlation between survivin and hsa-miR-542-3p. In SCC-15 cell line, inhibitor and mimic of hsa-miR-542-3p could regulate survivin expression. Plasma level of hsa-miR-542-3p was decreased in OSCC patients. The potential diagnostic performance of plasma hsa-miR-542-3p for OSCC patients was evaluated by ROC curve analysis and AUC was 0.82. CONCLUSIONS: Down-regulation of hsa-miR-542-3p correlates with increasing of survivin expression in OSCC. Plasma hsa-miR-542-3p is a potential diagnostic biomarker for OSCC.

Key words: Oral squamous cell carcinoma, MicroRNA, Survivin, Biomarker

中图分类号:

R739.8

吴宁, 陆瑛, 梁继宗. Survivin和hsa-miR-542-3p在口腔鳞状细胞癌中的表达和相关性分析[J]. 上海口腔医学, 2016, 25(6): 720-724.

WU Ning, LU Ying, LIANG Ji-zong. Expression and correlation of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2016, 25(6): 720-724.

参考文献

[1] 邱蔚六, 张震康, 张志愿, 等. 口腔颌面外科学 [M]. 第6版. 北京: 人民卫生出版社, 2008: 287-288.
[2] Massano J, Regateiro FS, Januário G, et al. Oral squamous cell carcinoma: review of prognostic and predictive factors [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 102 (1): 67-76.
[3] Lo Muzio L, Farina A, Rubini C, et al. Survivin as prognostic factor in squamous cell carcinoma of the oral cavity [J]. Cancer Lett, 2005, 225 (1): 27-33.
[4] Lo Muzio L, Pannone G, Staibano S, et al. Survivin expression in oral squamous cell carcinoma [J]. Br J Cancer, 2003, 89 (12): 2244-2248.
[5] Engels K, Knauer SK, Metzler D, et al. Dynamic intracellular survivin in oral squamous cell carcinoma: underlying molecular mechanism and potential as an early prognostic marker[J]. J Pathol, 2007, 211(5): 532-540.
[6] Jiang G, Li J, Zeng Z, et al. Lentivirus-mediated gene therapy by suppressing survivin in BALB/c nude mice bearing oral squamous cell carcinoma [J]. Cancer Biol Ther, 2006, 5(4): 435-440.
[7] Lu J, Getz G, Miska EA, et al. MicroRNA expression profiles classify human cancers [J]. Nature, 2005, 435 (7043): 834-838.
[8] Bartel DP. MicroRNAs: target recognition and regulatory functions [J]. Cell, 2009, 136(2): 215-233.
[9] Esquela-Kerscher A, Slack FJ. Oncomirs-microRNAs with a role in cancer [J]. Nat Rev Cancer, 2006, 6 (4): 259-269.
[10] Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method [J]. Nat Protoc, 2008, 3(6): 1101-1108.
[11] Hsu SD, Lin FM, Wu WY, et al. miRTarBase: a database curates experimentally validated microRNA-target interactions [J]. Nucleic Acids Res, 2011, 39(Database issue): D163-169.
[12] Wang X. miRDB: a microRNA target prediction and functional annotation database with a wiki interface [J]. RNA, 2008, 14 (6): 1012-1017.
[13] 毛兰萍, 葛振禄. 人口腔扁平苔藓及口腔鳞状细胞癌survivin表达的研究 [J].医药论坛杂志, 2011, 32(14): 22-24.
[14] Altieri DC. Survivin, cancer networks and pathway-directed drug discovery [J]. Nat Rev Cancer, 2008, 8(1): 61-70.
[15] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function [J]. Cell, 2004, 116 (2): 281-297.
[16] Yoon S, Choi YC, Lee S, et al. Induction of growth arrest by miR-542-3p that targets survivin [J]. FEBS Lett, 2010, 584 (18): 4048-4052.
[17] 蔡伟明, 彭仕骏, 吕文强, 等. 食道癌中miR-542-3p对Survivin基因的作用研究 [J].中国医药科学, 2011, 1(11): 18-19.
[18] Mitchell PS, Parkin RK, Kroh EM, et al. Circulating microRNAs as stable blood-based markers for cancer detection [J]. Proc Natl Acad Sci USA, 2008, 105(30): 10513-10518.
[19] Chen X, Ba Y, Ma L, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases [J]. Cell Res, 2008, 18(10): 997-1006.
[20] Wray NR, Yang J, Goddard ME, et al. The genetic interpretation of area under the ROC curve in genomic profiling [J]. PLoS Genet, 2010, 6(2): e1000864.