miR-142-3p通过靶向IRAK1抑制LPS诱导人牙周膜细胞的炎症反应

摘要/Abstract

摘要： 目的研究miR-142-3p过表达对脂多糖（LPS）诱导人牙周膜细胞（hPDLCs）分泌炎症因子的影响及其作用机制。方法组织块法培养原代正常hPDLCs,以不同浓度的LPS处理hPDLCs 6、12、24和48 h;ELISA检测miR-142-3p mimics对2.0 μg/mL LPS诱导的hPDLCs作用24 h后炎症因子（TNF-α、IL-6及IL-1β）的表达情况;双荧光素酶报告基因检测miR-142-3p与白介素1受体相关激酶1（interleukin-1receptor-associated-1 kinase1, IRAK1）的靶标关系,qRT-PCR检测miR-142-3p和IRAK1 mRNA的表达水平,Western 印迹法检测IRAK1、Toll样受体4（toll like receptor 4, TLR4）及核因子κB（nuclear factor-κB, NF-κB）的蛋白表达水平。采用SPSS 16.0软件包对数据进行统计学分析。结果2.0 μg/mL LPS作用24 h后,hPDLCs表达miR-142-3p最低,存在显著差异（P<0.01）;加入miR-142-3p mimics后,miR-142-3p表达量显著升高（P<0.01）;miR-142-3p过表达显著降低了炎症因子（TNF-α、IL-6及IL-1β）的表达水平（P<0.05）;双荧光素酶报告基因检测结果显示,miR-142-3p与IRAK1存在直接的靶标关系;miR-142-3p过表达可抑制IRAK1蛋白的表达水平（P<0.05）,但对IRAK1 mRNA表达水平无显著影响（P>0.05）;Western 印迹结果显示,miR-142-3p过表达显著下调TLR4蛋白和NF-κB蛋白磷酸化表达水平（P<0.05）。结论miR-142-3p过表达可减轻LPS诱导的hPDLCs炎症反应,其机制可能通过靶向抑制IRAK1进而抑制TLR4/NF-κB炎症信号通路,从而发挥对牙周组织的保护作用。

关键词: miR-142-3p, 牙周膜细胞, 炎症反应

Abstract: PURPOSE: To investigate the effects of miR-142-3p overexpression on secretion of inflammatory factors in human periodontal ligament cells (hPDLCs) induced by lipopolysaccharide (LPS) and its underlying mechanism. METHODS: hPDLCs were cultured by tissue cultivation in vitro and treated with various concentrations of LPS for 6, 12, 24 and 48 h. hPDLCs were treated with miR-142-3p mimics for 24 h in the presence of 2.0 μg/mL LPS and the expression levels of inflammatory factors (TNF-α, IL-6 and IL-1β) were detected by ELISA assay. The target relationship between miR-142-3p and interleukin-1 receptor- associated kinase 1 (IRAK1) was detected by dual-luciferase reporter assay. The miR-142-3p and IRAK1 mRNA expression was measured by qRT-PCR. The protein expression levels of IRAK1, TLR4 and NF-κB were detected by Western blotting. Statistical analysis was performed using SPSS 16.0 software package. RESULTS: The miR-142-3p expression level was decreased significantly at 24 h in 2.0 μg/mL LPS-treated hPDLCs (P<0.01). Transfection of miR-142-3p mimics remarkably increased miR-142-3p expression (P<0.01). The secretion of inflammatory factors (TNF-α, IL-6 and IL-1β) in LPS-treated hPDLCs was significantly decreased by miR-142-3p overexpression (P<0.05). Dual-luciferase reporter assay showed a directly targeting relationship between miR-142-3p and IRAK1. Overexpression of miR-142-3p significantly reduced the IRAK1 protein expression in LPS-treated hPDLCs (P<0.05), whereas no obvious effect on IRAK1 mRNA expression was noted (P>0.05). Moreover, miR-142-3p overexpression markedly diminished the protein expression of TLR4 and phosphorylated NF-κB in LPS-treated hPDLCs (P<0.05). CONCLUSIONS: Overexpression of miR-142-3p can alleviate the inflammatory response induced by LPS in hPDLCs and the underlying mechanism may be associated with targeting and inhibiting IRAK1 expression leading to suppression of TLR4/NF-κB inflammatory signaling pathway through which miR-142-3p overexpression protects peridentium against inflammation.

Key words: miR-142-3p, Human periodontal ligament cells, Inflammatory response

中图分类号:

R781.4

呼海燕, 王军强. miR-142-3p通过靶向IRAK1抑制LPS诱导人牙周膜细胞的炎症反应[J]. 上海口腔医学, 2016, 25(6): 682-687.

HU Hai-yan, WANG Jun-qiang. MiR-142-3p inhibits lipopolysaccharide-induced inflammatory response in human periodontal ligament cells through targeting IRAK1[J]. Shanghai Journal of Stomatology, 2016, 25(6): 682-687.

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