调节性T细胞和白介素15在口腔扁平苔藓中的表达及意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (4): 438-442.

调节性T细胞和白介素15在口腔扁平苔藓中的表达及意义

王翠杰¹, 李言君¹, 薛江南², 慈浩粟¹, 李丽萍¹, 李玲¹

1.滨州医学院附属医院口腔内科,山东滨州 256603; 2.滨州医学院免疫学教研室,山东烟台 264003

收稿日期:2015-10-09 出版日期:2016-08-25 发布日期:2016-09-06
通讯作者: 李言君,E-mail：Lyj6464@aliyun.com
作者简介:王翠杰（1990-）,女,硕士研究生,
基金资助:
国家自然科学基金(81273200)

Correlation of Treg and IL-15 expression in the peripheral blood of patients with oral lichen planus

WANG Cui-jie¹, LI Yan-jun¹, XUE Jiang-nan², CI Hao-su¹, LI Li-ping¹, LI Ling¹

1.Department of Oral Medicine, Affiliated Hospital of Binzhou Medical University. Binzhou 256603;
2.Department of Immunology, Binzhou Medical University. Yantai 264003, Shandong Province, China

Received:2015-10-09 Online:2016-08-25 Published:2016-09-06

摘要/Abstract

摘要： 目的研究外周血CD4⁺CD25⁺Foxp3⁺调节性T细胞(Treg细胞)和白介素15(IL-15)的表达在口腔扁平苔藓（OLP）发病机制中的作用。方法采用免疫荧光染色技术,经流式细胞仪检测36例口腔扁平苔藓患者和20例正常人外周血Treg表达水平。应用ELISA技术检测外周血IL-15的表达水平,采用SPSS16.0软件包对其表达差异进行统计学分析。结果口腔扁平苔藓患者外周血CD4⁺CD25⁺Foxp3⁺ Treg细胞数量较正常组显著上升,IL-15表达水平也显著高于正常组,但是口腔扁平苔藓糜烂型与非糜烂型之间的差异无显著性。Treg的表达与IL-15的水平呈正相关(P<0.05)。结论Treg和IL-15的表达上升可能是OLP的发病机制之一。

关键词: 口腔扁平苔藓, 调节性T细胞, 白介素15

Abstract: PURPOSE: To investigate the expression of Treg and IL-15 in the peripheral blood of patients with oral lichen planus (OLP). METHODS: The peripheral blood was obtained from 36 OLP patients and 20 healthy controls. Flow cytometry was used to evaluate the proportion of Treg cells, and the level of IL-15 was measured by ELISA. The data were analyzed by SPSS 16.0 software package. RESULTS: In the peripheral blood of OLP patients, the proportion of CD4⁺CD25⁺Foxp3⁺ Treg cells and IL-15 content were significantly higher than those of the healthy controls (P<0.05), but there were no significantly difference between two clinical subtypes of OLP(P>0.05). The proportion of CD4⁺CD25⁺Foxp3⁺Treg cells was positively correlated with the level of IL-15(r=0.70,P<0.05). CONCLUSIONS: The high expression of Treg cells and IL-15 in the perpheral blood of OLP patients may be responsible for the pathogenesis of OLP.

Key words: Oral lichen planus, Treg, IL-15

中图分类号:

R781.5

王翠杰, 李言君, 薛江南, 慈浩粟, 李丽萍, 李玲. 调节性T细胞和白介素15在口腔扁平苔藓中的表达及意义[J]. 上海口腔医学, 2016, 25(4): 438-442.

WANG Cui-jie, LI Yan-jun, XUE Jiang-nan, CI Hao-su, LI Li-ping, LI Ling. Correlation of Treg and IL-15 expression in the peripheral blood of patients with oral lichen planus[J]. Shanghai Journal of Stomatology, 2016, 25(4): 438-442.

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