颞下颌关节盘前移位后VEGF-C基因在髁突软骨中的表达及意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 136-140.

颞下颌关节盘前移位后VEGF-C基因在髁突软骨中的表达及意义

徐高丽^1*，汪淑华^1*，史秋涛^1*，柳毅^1*，阎帆^1*，徐婷²，谷志远¹

1.浙江中医药大学口腔医学院,浙江杭州 310053；
2.浙江大学医学院附属第一医院口腔科,浙江杭州 310003

收稿日期:2015-07-16 修回日期:2015-09-23 出版日期:2016-03-25 发布日期:2016-05-06
作者简介:徐高丽(1990-),女,在读硕士研究生,E-mail:852819264@qq.com;汪淑华(1989-),女,硕士,E-mail:385164787@qq.com。*并列第一作者谷志远,E-mail: gzy@zju.edu.cn
基金资助:
国家自然科学基金(81170979,81400533);浙江省医药卫生科技计划项目(2015KYB146)

The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance

XU Gao-li¹,WANG Shu-hua¹,SHI Qiu-tao¹,LIU Yi¹,YAN Fan¹,XU Ting²,GU Zhi-yuan¹

1.School of Stomatology, Zhejiang Chinese Medical University. Hangzhou 310053;
2.Department of Stomatology, First hospital, Zhejiang University School of Medicine. Hangzhou 310003, Zhejiang Province, China

Received:2015-07-16 Revised:2015-09-23 Online:2016-03-25 Published:2016-05-06

摘要/Abstract

摘要： 目的:探讨血管内皮细胞生长因子C在颞下颌关节盘前移位后髁突软骨中的表达及意义。方法:采用日本大耳白兔60只,48只用弹力丝牵拉关节盘前移,建立关节盘前移模型,分别于术后1、2、4、8周处死12只,12只空白对照组同期处死,取出颞下颌关节标本。利用基因芯片技术进行筛选,实时荧光定量PCR进行验证和SP免疫组化技术检测不同组内髁突软骨中VGEF-C的表达与分布。采用SPSS19.0软件包对每组样本实验组和对照组数据进行配对t检验。结果:颞下颌关节盘前移位后,在基因水平上VEGF-C相比于正常对照组均有不同程度变化,早期VEGF-C表达减少,第2周时降至最低水平,差异显著(P<0.05);第8周时,VEGF-C表达有所增加。镜下观察可见VEGF-C在髁突软骨增殖层、肥大层及钙化软骨层中均有表达,1周组阳性表达主要见于增殖深层和肥大层,钙化软骨层也有弱阳性表达。2周组和4周组阳性表达主要位于肥大层,且在钙化软骨层中表达逐渐增强。8周组肥大层有阳性表达,钙化软骨层未见明显阳性表达,8周组与对照组相比有显著差异(P<0.05)。结论:关节盘前移位后早期髁突软骨VEGF-C基因的转录与表达有所下降,而在后期升高, 可能参与关节盘移位后髁突软骨的改建。

关键词: 关节盘前移, 髁突, VEGF-C, 基因芯片

Abstract: PURPOSE: To investigate the expression and significance of vascular endothelial growth factor-C in the condylar cartilage after anterior disc displacement of temporomandibular joint. METHODS: Sixty healthy adult Japanese rabbits were used in this study. Forty-eight rabbits were subjected to surgical anterior disc displacement in the right TMJs, every 12 rabbits were sacrificed 1, 2, 4, 8 weeks post-operation, 12 rabbits without operation were used as normal control.The gene expression and distribution of VEGF-C in condylar cartilage were studied by gene chip, real-time quantitative PCR and SP immunohistochemical methods. Paired t test was used for analysis with SPSS19.0 software package. RESULTS: After anterior disc displacement, the expression of VEGF-C in the 2nd week reached to a minimum level, there was a significant difference (P<0.05), and increased in the 8th week. VEGF-C was expressed by cells of articular cartilage in the proliferative zone, hypertrophic zone and calcified cartilage zone. The expression in 1-week group was mainly found in the deep proliferative zone and hypertrophic zone, while the expression mainly found in the hypertrophic zone in 2-and 4-week group; calcified cartilage gradually enhanced, and there was significant difference (P<0.05) between 8-week group and control group. CONCLUSIONS: VEGF-C gene transcription and expression may be involved in the remodeling of the condylar cartilage after anterior disc displacement.

Key words: Anterior disc displacement, Mandibular condyle, VEGF-C , Gene chip

中图分类号:

R782.6

徐高丽，汪淑华，史秋涛，柳毅，阎帆，徐婷，谷志远. 颞下颌关节盘前移位后VEGF-C基因在髁突软骨中的表达及意义[J]. 上海口腔医学, 2016, 25(2): 136-140.

XU Gao-li,WANG Shu-hua,SHI Qiu-tao,LIU Yi,YAN Fan,XU Ting,GU Zhi-yuan. The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance[J]. Shanghai Journal of Stomatology, 2016, 25(2): 136-140.

参考文献

Kalaykova SI, Lobbezoo F, Naeije M. Risk factors for anterior disc displacement with reduction and intermittent locking in adolescents . J Orofac Pain, 2011, 25(2): 153-160.
Takahashi H, Shibuya M. The vascular endothelial growth factor (VEGF)/VEGF receptor system and its role under physiological and pathological conditions. Clin Sci (Lond), 2005, 109(3): 227-241.
Cébe-Suarez S, Zehnder-Fj llman A, Ballmer-Hofer K. The role of VEGF receptors in angiogenesis; complex partnerships . Cell Mol Life Sci, 2006, 63(5): 601-615.
Bluteau G, Julien M, Magne D, et al. VEGF and VEGF receptors are differentially expressed in chondrocytes . Bone, 2007, 40(3): 568-576.
Gu Z, Zhou Y, Zhang Y, et al. An animal model for inducing anterior disc displacement of the temporomandibular joint . J Orofac Pain, 2006, 20(2): 166-173.
Peppicelli S, Bianchini F, Calorini L, et al. Inflammatory cytokines induce vascular endothelial growth factor-C expression in melanoma-associated macrophages and stimulate melanoma lymph node metastasis . Oncol Lett, 2014, 8(3): 1133-1138
. Zhang Q, Lu Y, Proulx ST, et al. Increased lymphangiogenesis in joints of mice with inflammatory arthritis . Arthritis Res Ther, 2007, 9(6): R118. Zhang S, Cao W, Wei K, et al. Expression of VEGF-receptor in TMJ synovium of rabbit with experimentally induced internal derangement . Br J Oral Maxillofac Surg, 2013, 51(1): 69-73.
Tammela T, Zarkada G, Wallgard E, et al. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation . Nature, 2008, 454(7204): 656-660.
Benest AV, Harper SJ, Herttuala SY, et al. VEGF-C induced angiogenesis preferentially occurs at a distance from lypmphangiogenesis . Cardiovascular Res, 2008, 78(2): 315-323.
Wei L, Liu Y, Chen G, et al. Differentiation of lymphatic endothelial cells from bone marrow mesenchymal stem cells with VEGFs . Lymphology, 2012, 45(4): 177-187.
Dieterich LC, Seidel CD, Detmar M. Lymphatic vessel:new targets for the treatment of inflammatory diseases . Angiogenesis, 2014, 17(2): 359-371.
Zhou Q, Guo R, Wood R, et al. VEGF-C attenuates joint damage in chronic inflammatory arthritis by accelerating local lymphaticdrainage .Arthritis Rheum, 2011, 63(8): 2318-2328.
Kim H, Kataru RP, Koh GY. Inflammation-associated lymphangiogenesis: a double-edged sword . J Clin Invest, 2014, 124(3): 936-942.

[1]	刘丽敏, 孙晶晶, 钱佳骏, 张春叶, 胡宇华, 李江. 171例颌面部骨软骨瘤及恶变病例临床病理分析[J]. 上海口腔医学, 2024, 33(3): 324-327.
[2]	郁莹, 翁清清, 罗银月, 姚姝冉, 易芳羽, 张颖. 过量氟对小鼠髁突软骨损伤及自噬相关因子LC3、p62表达的影响[J]. 上海口腔医学, 2024, 33(2): 113-116.
[3]	应凯, 王亮, 朱永武, 李倩倩, 毛斌. 虚拟手术结合导板辅助7例髁突颈部骨折复位固定的效果评价[J]. 上海口腔医学, 2023, 32(1): 105-108.
[4]	沈刚. 含颌位因素错畸形与颌位重建基本原理[J]. 上海口腔医学, 2022, 31(6): 561-568.
[5]	张春香, 王天齐, 刘冬巧, 段小妮, 智铖, 王静, 林晨. 伴颞下颌关节紊乱病安氏Ⅱ类1分类错患者咀嚼运动中髁突轨迹分析[J]. 上海口腔医学, 2022, 31(4): 429-434.
[6]	陈旭, 徐丽丽, 蔡斌. 弹响消除试验在颞下颌关节可复性关节盘前移位诊断中的信度和效度分析[J]. 上海口腔医学, 2021, 30(6): 629-633.
[7]	陈龙, 代杰文, 沈国芳. Swell1基因在小鼠髁突软骨发育中的时空表达[J]. 上海口腔医学, 2021, 30(5): 462-466.
[8]	姚志涛, 安玮, 买买提吐逊·吐尔地. 保守治疗对儿童单侧髁突骨折患者颌骨对称性的影响[J]. 上海口腔医学, 2021, 30(1): 77-80.
[9]	雷杰, 肖遥, 罗茂璇, 徐晓梅. 12~14岁骨性Ⅱ类高角女性拔牙矫治前、后髁突位置与上气道结构变化[J]. 上海口腔医学, 2020, 29(3): 281-286.
[10]	李文艳, 陈文静, 侯伟, 秦金炜, 段义峰, 穆锦全. 不同骨型人群髁突不对称性的锥形束CT评价[J]. 上海口腔医学, 2019, 28(5): 523-528.
[11]	陈霜, 钟群, 翁蓓军, 叶页, 钱玉梅. 牙列重度磨耗患者咬合重建前、后颞下颌关节的线性测量[J]. 上海口腔医学, 2019, 28(3): 275-278.
[12]	王明锋, 李爽, 刘琳. 减小后牙▉平面倾斜度对安氏Ⅱ类高角畸形髁突位置影响的研究[J]. 上海口腔医学, 2018, 27(4): 386-389.
[13]	沈刚. SGTB 矫形诱发髁突改建的生物机制及临床意义[J]. 上海口腔医学, 2018, 27(3): 225-229.
[14]	刘帅, 赵震锦, 赵瑞, 郑雪松, 王丽萍. 下颌偏斜患者髁突位置的meta分析[J]. 上海口腔医学, 2018, 27(2): 210-215.
[15]	常忠福, 陈杰. 恒牙Angle Ⅲ类错伴颞下颌关节疾病患者髁突运动特征分析[J]. 上海口腔医学, 2016, 25(6): 738-743.