口腔鳞癌癌周组织流式细胞术分析及eIF4E的表达

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (5): 446-451.

口腔鳞癌癌周组织流式细胞术分析及eIF4E的表达

徐波, 程进强, 冉红兵

四川省攀枝花市中心医院口腔科,四川攀枝花 617000

收稿日期:2014-09-09 修回日期:2014-11-12 出版日期:2015-10-20 发布日期:2015-11-03
通讯作者: 徐波,E-mail: xubopzh@qq.com
作者简介:徐波（1975-）,男,硕士,主治医师

Flow cytometry analysis and eIF4E expression of para-tumor tissues of oral squamous cell carcinoma

XU Bo, CHEN Jin-qiang, RAN Hong-bing

Department of Stomatology, Panzhihua Central Hospital. Panzhihua 617000, Sichuan Province, China

Received:2014-09-09 Revised:2014-11-12 Online:2015-10-20 Published:2015-11-03

摘要/Abstract

摘要： 目的：对口腔鳞癌癌周组织中细胞的DNA倍性、S期比率（SPF）、增殖指数（PI）,以及eIF4E的表达进行分析,以探讨合理的口腔鳞癌手术安全切缘。方法将口腔鳞癌癌灶中心、癌周0~0.5 cm、0.5~1.0 cm、1.0~1.5 cm、1.5~2.0 cm组织标本分别设为T、P₁、P₂、P₃、P₄组。正常口腔黏膜标本设为N组。应用流式细胞仪对标本中DNA倍性、S期比率、增殖指数进行分析,采用免疫组化方法对标本中eIF4E的表达进行检测,并采用SPSS13.0软件包对数据进行统计学分析。结果在P₁、P₂、P₃、P₄组中,异倍体率、SPF 、PI呈逐渐下降趋势。N组均为二倍体,其异倍体率、SPF和PI与T、P₁、P₂、P₃、P₄组均有显著差异。eIF4E在P₁、P₂、P₃、P₄组中呈递减趋势,而在P₄组中阳性表达率仍达30%,与N组差异显著（P<0.01）。结论口腔鳞癌癌周组织的异倍体率、SPF 、PI各参数以及eIF4E的表达随着离癌灶距离的增加呈下降趋势,但到癌周2.0 cm时,仍然与正常口腔黏膜有显著差异。有条件时,手术要尽量将口腔鳞癌的手术切缘扩大到癌周2.0 cm以外;如无法达到,则需通过术后综合治疗防止肿瘤复发。

关键词: 口腔鳞癌, 癌周组织, eIF4E, 流式细胞术

Abstract: PURPOSE:To explore the surgical safe margin of oral squamous cell carcinoma (OSCC), the expression of eIF4E, DNA content and ploidy of para-tumor tissues were analyzed using immunohistochemistry and flow cytometry. METHODS: The specimens were embedded, cut and separated into 5 regions with the labels of T (center of the tumor), P₁ (0-0.5 cm to tumor center), P₂ (0.5-1 cm to tumor center), P₃ (1-1.5 cm to tumor center), and P₄(1.5-2 cm to tumor center). Twenty normal oral mucosal specimens were selected as control and labeled as N. Immunohistochemistry was employed to detect the expression of eIF4E. Moreover, flow cytometer (FCM) was used to assess the DNA content and type. The data was analyzed with SPSS13.0 software package. RESULTS: Ratio of heteroploidy, SPF and PI dropped gradually with the increment of distance of regions to tumor center. But, statistical differences were still observed between para-tumor tissues and normal tissues. eIF4E decreased from center to outer regions but still had 30% of significantly positive expression at P₄ region. CONCLUSIONS: Ratio of heteroploidy, SPF, PI and the expression of eIF4E dropped gradually with the increment of distance of regions to tumor center. Significant difference could still be noticed at the region 2 cm away from tumor tissues, which indicated that if possible 2 cm clinical boundary should be ensured during operation. Otherwise, post-operational chemical-and radio-therapies should be provided.

Key words: Oral squamous cell carcinoma, Para-tumor tissue, eIF4E, Flow cytometry

徐波, 程进强, 冉红兵. 口腔鳞癌癌周组织流式细胞术分析及eIF4E的表达[J]. 上海口腔医学, 2015, 24(5): 446-451.

XU Bo, CHEN Jin-qiang, RAN Hong-bing. Flow cytometry analysis and eIF4E expression of para-tumor tissues of oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2015, 24(5): 446-451.

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