局部注射重组人转化生长因子对大鼠正畸牙移动的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (4): 423-426.

局部注射重组人转化生长因子对大鼠正畸牙移动的影响

徐宇红¹, 李高华², 刘建国², 管晓燕²

1.遵义医学院第五附属(珠海)医院口腔科,广东珠海 519100;
2.遵义医学院附属口腔学院,贵州遵义 563003

收稿日期:2014-01-03 出版日期:2014-08-20 发布日期:2014-10-20
通讯作者: 徐宇红,Tel:0756-5102550,E-mail: xyhong-81@163.com
作者简介:徐宇红(1964-), 女, 学士, 副主任医师、副教授, 硕士研究生导师
基金资助:
贵州省教育厅科研基金(黔教科2008036)

Effect of local injection of rhTGF-β1 on osteoclasts during orthodontic tooth movement in rats

XU Yu-hong¹, LI Gao-hua², LIU Jian-guo², GUAN Xiao-yan²

1.Department of Dentistry, The Fifth Affiliated Hospital of Zunyi Medical College. Zhuhai 519100, Guangdong Province;
2.Stomatological Hospital of Zunyi Medical College. Zunyi 563003, Guizhou Province, China

Received:2014-01-03 Online:2014-08-20 Published:2014-10-20
Supported by:
Supported by Research Foundation of Department of Education of Guizhou Province (Qianjiaoke: 2008036).

摘要/Abstract

摘要： 目的:探讨局部注射重组人转化生长因子 (rhTGF -β1)对大鼠正畸牙移动速度的影响。方法:80只模型大鼠随机分为实验组和对照组,每组又按1、4、7、10和14 d再分为5个小组,每小组8只。实验组从加力的第1天开始,每2 d在大鼠加力侧上颌第一磨牙颊侧黏膜下注射rhTGF-β1 0.1 mL(5 ng/mL),对照组注射相同容量的PBS。加力后依实验设计时间分别处死每1小组大鼠。体视显微镜观察并采集图像,运用计算机图像分析软件测量牙移动的距离,同时应用TRAP组织化学染色观察不同时段压力侧破骨细胞数量的变化,采用SPSS 17.0 软件包对数据进行统计学分析。结果:局部注射rhTGF-β1的牙移动速度较对照组快,在加力后第7天,牙移动距离差异显著(P<0.05);第10天和第14天相比,差异显著(P<0.01)。实验组压力侧破骨细胞的TRAP阳性细胞数较对照组显著增多(P<0.01)。结论:局部注射rhTGF-β1增强了大鼠正畸牙破骨细胞的活性,促进了破骨细胞的骨吸收功能,加速了正畸牙移动。

关键词: 重组人转化生长因子β1, 破骨细胞, 口腔正畸, 牙移动

Abstract: PURPOSE: To investigate the effect of exogenous TGF-β1 on improving periodontal tissues remodeling during orthodontic tooth movement in rats. METHODS: Eighty male SD rats were randomly divided into 2 groups. Rats in the experimental group were injected respectively with rhTGF-β1 in dosages of 0.1 mL(5 ng/mL) in the buccal submucosal area of the molar every other day, while rats in the control group received equivalent volumes of PBS. Each group (n=40) was subdivided equally into 5 subgroups. An orthodontic appliance, consisting of a 5 mm nickel titanium closed coil spring, was ligated between the maxillary left incisor and first molar of each rat to deliver an initial force of 0.49N. Eight rats in each subgroup were sacrificed at one of the five time points(1, 4, 7, 10 and 14 days) after appliance placement. The distance of the tooth movement was measured by using stereomicroscope. Tissue sections around the first maxillary left molar were stained with tartrate-resistant acid phosphatase (TRAP) histochemistry to analyze the changes of the amount and distribution of osteoclasts on the compression side of tooth. Statistical analysis was performed using SPSS 17.0 software package. RESULTS: Molars treated with rhTGF-β1 moved mesially more rapidly than the control group. The distance of tooth movement of the experimental group showed a significant increase compared with the control group at day 7 (P<0.05) and significant increase compared with the control group at day 10 and 14 (P<0.01). The number of TRAP positive cells appearing in the periodontal ligament space on the pressure side of the alveolar bone were increased markedly in experimental group. There were statistically significant differences in the amount of osteoclasts between experimental and control groups (P<0.01). CONCLUSIONS: Local injection of rhTGF-β1 in the periodontal tissues can accelerate orthodontic tooth movement via enhancing the numbers of osteoclasts. At the histologic level, increased numbers of mononuclear osteoclasts are recruited and activated, resulting in greater amounts of alveolar bone resorption on the pressure side of the periodontal ligament.

Key words: Recombinant human transforming growth factor-β1, Osteoclasts, Orthodontics, Tooth movement

中图分类号:

R783.5

徐宇红, 李高华, 刘建国, 管晓燕. 局部注射重组人转化生长因子对大鼠正畸牙移动的影响[J]. 上海口腔医学, 2014, 23(4): 423-426.

XU Yu-hong, LI Gao-hua, LIU Jian-guo, GUAN Xiao-yan. Effect of local injection of rhTGF-β1 on osteoclasts during orthodontic tooth movement in rats[J]. Shanghai Journal of Stomatology, 2014, 23(4): 423-426.

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