血管内皮细胞对牙周组织细胞迁移的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (2): 172-178.

血管内皮细胞对牙周组织细胞迁移的影响

丁进, 尹元正, 黄佳妮, 胡琮佼

上海交通大学医学院附属第九人民医院口腔医学院牙周病科;
上海市口腔医学重点实验室, 上海 200011

收稿日期:2013-05-17 出版日期:2014-04-20 发布日期:2014-05-21
通讯作者: 尹元正, Tel: 021-23271699-5282, E-mail:yinyuanzheng@gmail.com
作者简介:丁进(1987-), 男, 硕士, 住院医师, E-mail:cqxiaoding2009@126.com

The effect of vascular endothelial cells on the migration of periodontal ligament cells and gingival fibroblasts

DIN Jin, YIN Yuan-zheng, HUANG Jia-ni, HU Cong-jiao

Department of Periodontics, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;
Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2013-05-17 Online:2014-04-20 Published:2014-05-21

摘要/Abstract

摘要： 目的: 通过血管内皮细胞与牙周组织细胞的复合培养模拟牙周组织再生环境, 研究血管内皮细胞对牙周组织细胞迁移的影响, 为明确血管内皮细胞在牙周组织再生过程中的作用奠定基础。方法: 应用原代培养的血管内皮细胞, 在Transwell嵌套中实现与人牙周膜成纤维细胞、牙龈成纤维细胞间的复合培养。分别以Transwell嵌套滤膜下腔面24 h的细胞数量检测垂直迁移能力, 以划痕实验后0、8、16和24 h计数划痕区域的细胞数量检测水平迁移能力, 以玻片试验及计算机辅助图像处理软件计算实验第1、4和7天细胞覆盖创面的面积检测覆盖创面能力, 并均与单独培养的2种牙周组织细胞作为对照。采用SPSS13.0软件包对实验结果进行统计学分析。结果: 血管内皮细胞存在时, 2种牙周细胞在垂直和水平方向的迁移量均显著高于单独培养组(P<0.01), 且牙周膜成纤维细胞垂直向的迁移量显著高于牙龈成纤维细胞(P<0.01), 但其水平迁移量在实验24 h时则显著低于牙龈成纤维细胞(P<0.01);在血管内皮细胞共存条件下, 2种牙周细胞创面覆盖能力在实验第7天高于对照组, 且牙周膜成纤维细胞的创面覆盖能力的增加显著高于牙龈成纤维细胞(P<0.01)。结论: 血管内皮细胞对牙周膜、牙龈成纤维细胞的迁移和创面覆盖有明显促进作用, 且血管内皮细胞对牙周膜成纤维细胞的垂直向迁移和创面覆盖能力的促进作用更为明显。

关键词: 牙周再生, 血管内皮细胞, 细胞迁移

Abstract: PURPOSE: To investigate the effect of human vascular endothelial cells (HUVEC) on the migration of human periodontal ligament cells (HPDLC) and human gingival fibroblasts (HGF) through a model mimicking periodontal wound healing or regeneration. METHODS: HUVEC, HPDLC and HGF were co-cultured using a Transwell system, while HPDLC or HGF was cultured independently as control. Chemotaxis assay was performed using cell culture inserts (0.8μm pore size) to assess the effect of HUVEC on HPDLC and HGF vertical migration at 24h. A scratch wound assay was performed to assess the effect of HUVEC on HPDLC and HGF horizontal migration at 0 h, 8 h, 16 h and 24 h. A round glass sheet assay was performed to assess the effect of HUVEC on HPDLC and HGF wound healing ability at 1d, 4d and 7d. The data was processed with SPSS 13.0 software package. RESULTS: At the round glass sheet assay, while HUVEC was present, the integrated option density (IOD) of HPDLCs and HGFs were significantly higher than each of the single culture (P<0.01). Results of chemotaxis assay and scratch wound assay showed that the cell number of HPDLCs and HGFs were significantly higher than each of the single culture while HUVEC was present (P<0.01), and the number of PHDLCs was significantly higher than HGFs on chemotaxis assay (P<0.01) but the result of scratch wound assay was on the contrary (P<0.01). CONCLUSIONS: The presence of HUVEC can promote migration of periodontal cells, and the effect is more prominent on HPDLC than on HGF in vertical migration and wound healing, while horizontal migration is more prominent on HGFs as contrast.

Key words: Periodontal regeneration, Vascular endothelial cell, Cell migration

中图分类号:

R781.4

丁进, 尹元正, 黄佳妮, 胡琮佼. 血管内皮细胞对牙周组织细胞迁移的影响[J]. 上海口腔医学, 2014, 23(2): 172-178.

DIN Jin, YIN Yuan-zheng, HUANG Jia-ni, HU Cong-jiao. The effect of vascular endothelial cells on the migration of periodontal ligament cells and gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2014, 23(2): 172-178.

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