纳米氟磷灰石聚醚醚酮种植体骨界面的定量分析

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (2): 166-171.

纳米氟磷灰石聚醚醚酮种植体骨界面的定量分析

李凌宇¹, 周聪颖¹, 魏杰², 马健¹

1.同济大学口腔医学院口腔生物医学及转化医学实验室, 上海 200072;
2.华东理工大学材料学院, 上海 200237

收稿日期:2013-07-22 出版日期:2014-04-20 发布日期:2014-05-21
通讯作者: 马健, Tel:021-66311632, E-mail: jian.ma@tongji.edu.cn
作者简介:李凌宇(1987-), 女, 硕士, 医师, E-mail:lingyu.li@hotmail.com

Quantitative analysis of nFA/PEEK implant interfaces in Beagle dogs

LI Ling-yu¹, ZHOU Cong-ying¹, WEI Jie², MA Jian¹

1.School of Stomatology, Laboratory of Oral Biomedical Science and Translational Medicine, Tongji University. Shanghai 200072;
2.Institute of Materials Science, East China University of Science and Technology. Shanghai 200237, China

Received:2013-07-22 Online:2014-04-20 Published:2014-05-21

摘要/Abstract

摘要： 目的: 通过犬动物实验, 检测喷砂处理螺纹柱状纳米氟磷灰石聚醚醚酮材料(nFA/PEEK)的生物相容性和成骨性能。方法: 将喷砂和非喷砂nFA/PEEK各10颗, 延期植入6只犬下颌前磨牙区, 术后8周和12周末各随机处死3只, 种植体骨标本行同步辐射扫描后, 制作非脱钙种植骨组织切片, 四环素荧光标记测定动态参数骨结合率(BIC)和矿化沉积率(MAR)。苦味酸品红染色测定骨的静态参数。采用SPSS18.0软件包对数据进行统计学分析。结果: 同步辐射扫描8周, 种植体骨床界面呈现连续接触, 新生骨组织长入螺纹凹陷处;12周均基本形成骨结合, 喷砂组新骨形成和成熟更快。8周末的BIC(63.34%)和MAR(1.51 μm/d)均显著高于(P<0.05)未喷砂组种植体(22.97%, 1.34 μm/d);12周末喷砂组的BIC(75.49%)显著高于(P>0.05)未喷砂组 (55.35%), 但MAR (1.28 μm/d 对1.27 μm/d) 无显著差异。骨静态学参数喷砂组骨体积分数和骨小梁数量显著高于非喷砂组, 但骨小梁间隔显著低于非喷砂组(P<0.05), 表明喷砂组更有利于骨量的增加, 但骨小梁厚度无显著差异。结论: 本研究表明, 喷砂处理nFA/PEEK种植体具有良好的生物相容性, 与其骨床结合紧密。

关键词: 纳米氟磷灰石聚醚醚酮, 喷砂, 骨形态计量学

Abstract: PURPOSE: A new synthetic nano-fluorapatite poly-ether-ether-ketone (nFA/PEEK) dental implant in screw type was developed and its biocompatibility and osseointegration ability were evaluated in Beagle dogs. METHODS: Ten sandblasted and 10 non-sandblasted nFA/PEEK implants were placed in 6 adult Beagle dogs and the bilateral mandibular second and third premolar was extracted and the wounds were healed over 3 months. Three dogs were randomly chosen and executed at the end of 8th and 12th week. To evaluated bone to implant contact(BIC) and mineral apposition rate(MAR), tetracycline was intramuscularly given twice at 10th and 3rd day, before sacrifice respectively. The implants and the surrounding bone as a whole were collected and examined by synchrotron radiation tomography. Undecalcified implant and bone specimens were cut and stained by Van Gieson for a static histomorphometric analysis. The data was analyzed using SPSS18.0 software package. RESULTS: The implants were demonstrated by synchrotron radiation and connected to most of their bone beds at 8th week. Some new bones were developed into threads of screw implants, while complete osseointegration were noted at 12th week. Sandblasted implants were found to be faster matured. BIC and MAR of sandblasted group (63.34%, 1.51 μm/d) were significantly higher than that of non-sandblasted group (22.97%, 1.34 μm/d) (P<0.05) at 8th week. BIC of sandblasted group (75.49%) was significantly higher than that of non-sandblasted (55.35%) (P<0.05) at 12th week, while MAR was not significantly different between the 2 groups (1.28 μm/d versus 1.27 μm/d). The static parameters showed that bone volume fraction (BV/TV) and trabecular number (Tb.N) of sandblasted group were significantly higher than that of non-sandblasted group, while trabecular spacing (Tb.Sp) was significantly lower (P<0.05); No significant difference was found in trabecular thickness (Tb.Th) between 2 groups (P>0.05). The sandblasting group was more conductive to increase their bone mass. CONCLUSIONS: Sandblasted synthetic polymer’s biomaterial nFA/PEEK has excellent biocompatibility and osteogenic properties, which is better osseointegrated to its bone bed. It will be a promising material as dental implants in the future.

Key words: Dental implants, Sandblasting, Histomorphometry

中图分类号:

R783.1

李凌宇, 周聪颖, 魏杰, 马健. 纳米氟磷灰石聚醚醚酮种植体骨界面的定量分析[J]. 上海口腔医学, 2014, 23(2): 166-171.

LI Ling-yu, ZHOU Cong-ying, WEI Jie, MA Jian. Quantitative analysis of nFA/PEEK implant interfaces in Beagle dogs[J]. Shanghai Journal of Stomatology, 2014, 23(2): 166-171.

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