骨桥蛋白、TGF-β1及TNF-α在舍格伦综合征中的表达及相关分析

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (3): 322-325.

骨桥蛋白、TGF-β1及TNF-α在舍格伦综合征中的表达及相关分析

孙巍^1,2，郁利²，魏本娟²，周海文¹

1.上海交通大学医学院附属第九人民医院?口腔医学院口腔黏膜病科，上海市口腔医学重点实验室，上海 200011
2.上海交通大学医学院附属仁济医院口腔科，上海 200127

收稿日期:2013-02-19 修回日期:2013-04-15 出版日期:2013-06-10 发布日期:2013-06-10
通讯作者: 周海文, Tel:021-23271699-5696,E-mail:haiwen39@hotmail.com
作者简介:孙巍(1976- )，男，在读硕士研究生，主治医师，E-mail：dentist1609@hotmail.com

The function of osteopontin and other cytokines in the diagnosis of SjÖgren’s syndrome

SUN Wei^1,2, YU Li², WEI Ben-juan², ZHOU Hai-wen¹

1.Department of Oral Mucosal Disease, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
2. Department of Stomatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200127, China

Received:2013-02-19 Revised:2013-04-15 Online:2013-06-10 Published:2013-06-10

摘要/Abstract

摘要： 目的：研究舍格伦综合征(Sj?gren’s syndrome，SS)患者血清中骨桥蛋白(osteopontin,OPN)、TNF-α、TGF-β1三者间的相关性，为临床诊断提供依据。方法：选择经病理确诊的SS患者40例，其中原发性SS 15例，继发性SS 25例。ELISA检测患者血清中骨桥蛋白(OPN)、TNF-α、TGF-β1的含量，并利用SPSS13.0软件包对数据进行统计学分析。结果：OPN 和TNF-α在患者血清中的含量较正常人显著增高(P<0.05)，继发性SS的TGF-β1血清含量显著高于原发性SS(P<0.05)，TGF-β1的血清含量与正常对照组相比无显著差异。OPN与TGF-β1具有相关性。结论：OPN和TNF-α可作为SS检测的辅助手段，而TGF-β1可作为鉴别继发性SS和原发性SS的一种检测指标。OPN、TGF-β1在SS的发病过程中各自发挥作用的同时，还相互协调，相互牵制，充分体现了细胞因子网络的双向性、多样性和整体性。

关键词: 舍格伦综合征, OPN, TGF-β1, TNF-α

Abstract: PURPOSE: To explore the relationship of serum concentrations of osteopontin(OPN), TNF-α and TGF-β1 in SjÖgren’s syndrome patients. METHODS: Forty [SjÖgren’s] syndrome (SS) patients were determined by pathological examination, which included 15 primary SS and 25 secondary SS. Serum level of OPN, TNF-alpha and TGF-beta 1 were examined by enzyme linked immunosorbent assay (ELISA). Multiple correlation analysis was performed using SPSS13.0 software package. RESULTS: Serum OPN, TNF-alpha levels increased in SS compared with healthy subjects (P<0.05).Serum TGF-beta 1 level in secondary SS significantly increased than primary SS (P<0.05). On the contrary, serum TGF-beta1 level had no significant change compared with the controls. OPN was correlated with TGF-beta 1. CONCLUSIONS: OPN and TNF-alpha may be used as adjunctive diagnostic parameter. TGF-beta 1 can be used as an index in differential diagnosis of primary and secondary SS. OPN and TGF-beta 1 have different effects and interactions between them, demonstrating mutuality, diversity and integrity of the cytokine network.

Key words: SjÖgren’s syndrome, Osteopontin, Immunohistochemistry, ELISA

中图分类号:

R781.5

孙巍, 郁利, 魏本娟, 周海文. 骨桥蛋白、TGF-β1及TNF-α在舍格伦综合征中的表达及相关分析[J]. 上海口腔医学, 2013, 22(3): 322-325.

SUN Wei, YU Li, WEI Ben-juan, ZHOU Hai-wen. The function of osteopontin and other cytokines in the diagnosis of SjÖgren’s syndrome[J]. Shanghai Journal of Stomatology, 2013, 22(3): 322-325.

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