不同患龋水平儿童菌斑生物膜间代谢物差异分析

doi:10.19439/j.sjos.2024.05.009

上海口腔医学 ›› 2024, Vol. 33 ›› Issue (5): 492-499.doi: 10.19439/j.sjos.2024.05.009

不同患龋水平儿童菌斑生物膜间代谢物差异分析

杨园梦^1#, 何德亿^1#, 卢展民², 孟雯¹, 陈昕煜¹, 王阅¹, 李飞^1,*, 陆海霞^1,*

1.上海交通大学医学院附属第九人民医院口腔预防科, 上海交通大学口腔医学院, 国家口腔医学中心, 国家口腔疾病临床医学研究中心, 上海市口腔医学重点实验室, 上海市口腔医学研究所, 上海 200011;
2.香港大学牙医学院, 香港 999077

收稿日期:2024-03-07 修回日期:2024-04-10 出版日期:2024-10-25 发布日期:2024-11-14
通讯作者: 陆海霞,E-mail: ritalu0225@hotmail.com;李飞,E-mail: charlse123@163.com。^*共同通信作者
作者简介:杨园梦（1998-）,女,硕士,E-mail: yang861161559@qq.com;何德亿（1996-）,男,硕士,E-mail: Jackson_pieces96@hotmail.com。<sup>#</sup>并列第一作者
基金资助:
上海市卫健委项目（201940041）; 上海市科委港澳台科技合作项目（22410760200）

Metabolites differences between plaque biofilms of children with different levels of dental caries

YANG Yuan-meng¹, HO Teck-ek¹, LO Edward Chin Man², MENG Wen¹, CHEN Xin-yu¹, WANG Yue¹, LI Fei¹, LU Hai-xia¹

1. Department of Preventive Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011;
2. Faculty of Dentistry, The University of Hong Kong. Hong Kong 999077, China

Received:2024-03-07 Revised:2024-04-10 Online:2024-10-25 Published:2024-11-14

摘要/Abstract

摘要： 目的： 比较不同患龋水平儿童菌斑生物膜内代谢物差异。方法： 采用病例-对照研究,根据国际龋病检测和评估记录的儿童患龋状况,将36个月龄学龄前儿童分为无龋组、低龋组和高龋组,收集牙菌斑生物膜样本。通过非靶标代谢组学技术检测3组菌斑生物膜代谢物成分,联合正交偏最小二乘判别分析、火山图、热图等方法比较不同患龋水平儿童菌斑生物膜间代谢物差异。采用SPSS 26.0 软件包对数据进行统计学分析。结果： 纳入102名儿童,无龋、低龋和高龋组各34名。在菌斑生物膜中共检测到660种代谢物,属于11个类别。6种代谢物在低龋和无龋组间存在统计学差异,22种代谢物在高龋和无龋组间有统计学差异（P<0.05）。半胱氨酸-半胱氨酸-半胱氨酸-谷氨酰胺、半胱氨酸-谷氨酰胺-半胱氨酸-半胱氨酸、5-氧-对香豆酰黑木素是不同患龋水平儿童组间的共同差异代谢物。结论： 患龋儿童与健康儿童菌斑生物膜代谢物组成不同。肽类物质是不同菌斑生物膜间的重要差异代谢物,与口腔健康状态密切相关。

关键词: 低龄儿童龋, 代谢组学, 菌斑生物膜, 代谢物

Abstract: PURPOSE: To explore the differences between plaque biofilms of children with different caries activities via metabolomics. METHODS: A case-control study was conducted to investigate the oral health-related behaviors of children in caries-free (CF), low level of early childhood caries(LECC) and high level of early childhood caries (HECC) groups and to collect supragingival plaque biofilms. Untargeted metabolomics was used to detect the compositions of plaque biofilm metabolites in three groups. Orthogonal partial least squares discriminant analysis, volcano plot and heat map were used to analyze the significant differential metabolites between groups with SPSS 26.0 software package. RESULTS: A total of 102 children aged 36 months were included in this study, including 34 children in each group. A total of 660 metabolites belonging to 11 categories were detected. There were statistical differences of 6 metabolites between LECC group and CF group, and 22 metabolites showed statistical differences between HECC group and CF group(P<0.05). Cys Cys Cys Gln, Cys Gln Cys Cys and 5-O-p-coumaroylnigrumin were the common differential metabolites between children with different levels of dental caries. CONCLUSIONS: There are differences in the compositions of plaque biofilms between children with different caries activities and healthy children. Peptides are important differential metabolites within plaque biofilms, also related to oral health.

Key words: Early childhood caries, Metabolomics, Plaque biofilm, Metabolites

中图分类号:

R780.1

杨园梦, 何德亿, 卢展民, 孟雯, 陈昕煜, 王阅, 李飞, 陆海霞. 不同患龋水平儿童菌斑生物膜间代谢物差异分析[J]. 上海口腔医学, 2024, 33(5): 492-499.

YANG Yuan-meng, HO Teck-ek, LO Edward Chin Man, MENG Wen, CHEN Xin-yu, WANG Yue, LI Fei, LU Hai-xia. Metabolites differences between plaque biofilms of children with different levels of dental caries[J]. Shanghai Journal of Stomatology, 2024, 33(5): 492-499.

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不同患龋水平儿童菌斑生物膜间代谢物差异分析

Metabolites differences between plaque biofilms of children with different levels of dental caries

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