USP20在口腔鳞癌中的表达及临床意义

doi:10.19439/j.sjos.2024.05.004

上海口腔医学 ›› 2024, Vol. 33 ›› Issue (5): 466-470.doi: 10.19439/j.sjos.2024.05.004

USP20在口腔鳞癌中的表达及临床意义

翟小奇, 刘搏, 沈程奕, 侯睿, 唐超男, 关键

佳木斯大学附属口腔医院颌面外科, 黑龙江佳木斯 154004

收稿日期:2024-06-20 修回日期:2024-07-22 出版日期:2024-10-25 发布日期:2024-11-14
通讯作者: 关键,E-mail: guanjian1207@163.com
作者简介:翟小奇（1997-）,男,汉族,硕士,住院医师,E-mail: drxiaoqizhai@163.com
基金资助:
黑龙江省卫生健康委科研课题（2020-319）

Expression and clinical significance of USP20 in oral squamous cell carcinoma

ZHAI Xiao-qi, LIU Bo, SHENG Cheng-yi, HOU Rui, TANG Chao-nan, GUAN Jian

Department of Maxillofacial Surgery, Jiamusi University Affiliated Stomatological Hospital. Jiamusi 154004, Heilongjiang Province, China

Received:2024-06-20 Revised:2024-07-22 Online:2024-10-25 Published:2024-11-14

摘要/Abstract

摘要： 目的： 探讨泛素特异性蛋白酶20（ubiquitin specific protease 20, USP20）在口腔鳞癌（oral squamous cell carcinoma, OSCC）中的表达及与临床病理指标的关系。方法： 筛选癌症基因组图谱(the cancer genome atlas,TCGA)数据库中OSCC样本及配对癌旁样本,分别进行差异表达分析和配对差异分析。通过免疫组织化学SABC法检测组织芯片OSCC及癌旁和正常组织中USP20的表达水平,分析其与临床病理指标间的相关性。采用GraphPad Prism 9.5.0软件对数据进行统计学分析。结果： TCGA数据显示,USP20在OSCC中的表达显著高于癌旁组织(P<0.001)。免疫组织化学染色评分显示,USP20在OSCC中的表达显著高于癌旁组织和正常组织(P<0.01);组织芯片临床病理指标分析显示,USP20的染色评分在肿瘤不同分化程度(P<0.05)及原发灶大小(P<0.01)的差异具有统计学意义。Kaplan-Meier分析显示,高表达组与低表达组总体生存时间及5年生存率相比无统计学差异(P>0.05)。结论： USP20可能参与OSCC的发生与进展,与肿瘤分化存在相关性,可加速其生长。

关键词: 口腔鳞癌, USP20, 生物信息学, 免疫组织化学, 临床病理指标

Abstract: PURPOSE: To investigate the expression of ubiquitin specific protease 20 (USP20) in oral squamous cell carcinoma (OSCC) and its correlation with clinicopathological parameters. METHODS: OSCC samples and paired paracancerous samples were screened from the TCGA database for differential expression analysis and paired differential analysis. Immunohistochemical staining was used to detect the expression level of USP20 in OSCC tissue, paracancerous tissues and normal tissues. The correlation between USP20 expression level and clinicopathological parameters of tissue microarray was analyzed. GraphPad Prism 9.5.0 software was used for statistical analysis of the data. RESULTS: TCGA data showed that the expression of USP20 in OSCC was significantly higher than that in paracancerous tissue(P<0.001). Immunohistochemical staining score showed that the expression of USP20 in OSCC was significantly higher than that in paracancerous tissues and normal tissues(P<0.01). The analysis of clinicopathological indicators of tissue microarray showed that the staining scores of USP20 were significantly different in tumor differentiation (P<0.05) and primary tumor size (P<0.01). Kaplan-Meier survival analysis indicated no significant difference in overall survival time and 5-year survival rate between high expression group and low expression group (P>0.05). CONCLUSIONS: USP20 is highly expressed in OSCC compared with paracancerous tissues and normal oral tissues. The expression of USP20 is associated with differentiation and the size of primary tumor in OSCC,and can accelerate its growth.

Key words: Oral squamous cell carcinoma, USP20, Bioinformatics, Immunohistochemistry, Clinicopathological indicators

中图分类号:

R739.8

翟小奇, 刘搏, 沈程奕, 侯睿, 唐超男, 关键. USP20在口腔鳞癌中的表达及临床意义[J]. 上海口腔医学, 2024, 33(5): 466-470.

ZHAI Xiao-qi, LIU Bo, SHENG Cheng-yi, HOU Rui, TANG Chao-nan, GUAN Jian. Expression and clinical significance of USP20 in oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2024, 33(5): 466-470.

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USP20在口腔鳞癌中的表达及临床意义

Expression and clinical significance of USP20 in oral squamous cell carcinoma

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