基于16S rDNA高通量测序技术的重度低龄儿童龋患者口腔微生物及肠道菌群群落分析

doi:10.19439/j.sjos.2024.02.010

上海口腔医学 ›› 2024, Vol. 33 ›› Issue (2): 164-169.doi: 10.19439/j.sjos.2024.02.010

基于16S rDNA高通量测序技术的重度低龄儿童龋患者口腔微生物及肠道菌群群落分析

李杨^1,2, 赵梦珺^1,3, 卢红巧¹, 刘建国^1,2, 刘云坤^1,4, 吴家媛^1,2

1.遵义医科大学附属口腔医院,贵州遵义 563000;
2.贵州省普通高等学校口腔疾病研究特色重点实验室,贵州遵义 563000;
3.无锡口腔医院,江苏无锡 214000;
4.口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心, 四川大学华西口腔医院,四川成都 610041

收稿日期:2023-04-04 修回日期:2023-06-10 出版日期:2024-04-25 发布日期:2024-05-14
通讯作者: 吴家媛,E-mail: wujiayuan@zmu.edu.cn
作者简介:李杨（1982-）,女,硕士,副主任医师,E-mail: 564015299@qq.com
基金资助:
贵州省卫生健康委科学技术基金项目（gzwjkj2020-1-163）; 贵州省科技计划项目(黔科合基础-ZK[2022]一般638); 遵义市科技计划项目[遵市科合HZ字(2020)293号]

Analysis of saliva and intestinal microbial community in children with severe caries based on 16S rDNA high-throughput sequencing technology

LI Yang^1,2, ZHAO Meng-jun^1,3, LU Hong-qiao¹, LIU Jian-guo^1,2, LIU Yun-kun^1,4, WU Jia-yuan^1,2

1. Hospital of Stomatology, Zunyi Medical University. Zunyi Guizhou 563000, Guizhou Province;
2. Guizhou Provincial Key Laboratory of Oral Disease Research in College and University. Zunyi 563000, Guizhou Province;
3. Wuxi Stomatology Hospital. Wuxi 214000, Jiangsu Province;
4. State Key Laboratory of Oral Diseases; National Clinical Research Center for Oral Diseases; West China Hospital of Stomatology, Sichuan University. Chengdu 610041, Sichuan Province, China

Received:2023-04-04 Revised:2023-06-10 Online:2024-04-25 Published:2024-05-14

摘要/Abstract

摘要： 目的: 应用16S rDNA高通量测序,分析高龋及无龋儿童口腔和肠道微生物群落构成特征。方法: 从本团队前期流行病学调查的431名遵义市3~5岁儿童中,随机抽取高龋和无龋儿童各25名,采集其唾液及粪便样本。采用16S rDNA测序技术分析样本菌群结构,比较口腔和肠道微生物菌群的构成差异。采用SPSS 18.0软件包对结果进行统计学分析。结果: 高龋组儿童肠道菌群多样性显著高于无龋组(P<0.05)。高龋组儿童唾液菌群多样性较无龋组高,但差异无统计学意义(P>0.05)。在门水平上,高龋组和无龋组儿童的肠道及口腔菌群物种差异无显著性。在属水平上,无龋组肠道中的Blautia、[Eubacterium] hallii group、[Eubacterium] eligens group显著高于高龋组(P<0.05),Parasutterella、Christensenellaceae R-7 group显著低于高龋组(P<0.05);无龋组唾液中的Peptostreptococcus显著高于高龋组(P<0.05);无龋组唾液中的Dialister、Kingella、Escherichia-Shigella、Treponema显著低于高龋组(P<0.05)。结论: 高龋儿童肠道菌群中物种多样性高于无龋儿童。高龋儿童和无龋儿童的口腔和肠道微生物组成在属水平上存在显著差异性菌种。

关键词: 高通量测序, 低龄儿童龋, 微生物群落, 肠道菌群失衡

Abstract: PURPOSE: The characteristics of saliva and intestinal microbial community in children with high caries and no caries were analyzed by 16S rDNA high-throughput sequencing. METHODS: Among 431 children aged 3-5 years old in Zunyi City who were investigated previously by our team, 25 children in the high caries group and the same in the caries-free group were selected for fecal and saliva samples. 16S rDNA high-throughput sequencing was used to analyze the bacterial flora structure of the samples and identify the species with different relative abundance at the species level. SPSS 18.0 software package was used for data analysis. RESULTS: The diversity of intestinal flora in the high caries group was higher than that in the caries-free group, and the difference was statistically significant(P<0.05). The diversity of salivary flora in the high caries group was more than that in the caries-free group, with no significant difference(P>0.05). At phylum level,there was no significant difference in intestinal and salivary flora between children with high caries and children without caries. At gene level, Blautia, [Eubacterium] hallii group and [Eubacterium] eligens group in the intestine of caries-free group were significantly higher than those of high caries group(P<0.05), while Parasutterella and Christensenellaceae R-7 group were significantly lower than those of high caries group(P<0.05). At gene level, Peptostreptococcus in saliva of caries-free group was significantly higher than that in high caries group(P<0.05). Dialister, Kingella, Escherichia-Shigella and Treponema in saliva of caries-free group were significantly lower than those in high caries group(P<0.05). CONCLUSIONS: There are significant differences in species composition of intestinal flora but no in salivary flora between children with high caries and children without caries.

Key words: High-throughput sequencing, Early childhood caries, Microbial community, Intestinal dysbacteriosis

中图分类号:

R788.1

李杨, 赵梦珺, 卢红巧, 刘建国, 刘云坤, 吴家媛. 基于16S rDNA高通量测序技术的重度低龄儿童龋患者口腔微生物及肠道菌群群落分析[J]. 上海口腔医学, 2024, 33(2): 164-169.

LI Yang, ZHAO Meng-jun, LU Hong-qiao, LIU Jian-guo, LIU Yun-kun, WU Jia-yuan. Analysis of saliva and intestinal microbial community in children with severe caries based on 16S rDNA high-throughput sequencing technology[J]. Shanghai Journal of Stomatology, 2024, 33(2): 164-169.

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基于16S rDNA高通量测序技术的重度低龄儿童龋患者口腔微生物及肠道菌群群落分析

Analysis of saliva and intestinal microbial community in children with severe caries based on 16S rDNA high-throughput sequencing technology

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