上海口腔医学

上海口腔医学 ›› 2024, Vol. 33 ›› Issue (2): 130-134.doi: 10.19439/j.sjos.2024.02.004

• 论著 • 上一篇    下一篇

阿托伐他汀促进大鼠牙槽骨缺损愈合及对Wnt/β-catenin信号通路的影响

陈宏丽1, 尹刚2, 张海娟3   

  1. 1.山西医科大学汾阳学院 口腔医学教研室,山西 汾阳 032200;
    2.山西省汾阳医院 口腔科,山西 汾阳 032200;
    3.山西医科大学汾阳学院 病理生理学教研室,山西 汾阳 032200
  • 收稿日期:2023-03-14 修回日期:2023-05-05 出版日期:2024-04-25 发布日期:2024-05-14
  • 通讯作者: 陈宏丽,E-mail：chenhongli_790217@163.com
  • 作者简介:陈宏丽（1979-）,女,硕士,副教授
  • 基金资助:
    山西省重点研发计划（指南）项目（201603D321084）

Atorvastatin promotes the healing of alveolar bone defect in rats and its effect on Wnt/β-catenin signaling pathway

CHEN Hong-li1, YIN Gang2, ZHANG Hai-juan3   

  1. 1. Department of Stomatology, Fenyang College of Shanxi Medical University. Fenyang 032200;
    2. Department of Stomatology, Fenyang Hospital. Fenyang 032200;
    3. Department of Pathophysiology, Fenyang College of Shanxi Medical University. Fenyang 032200, Shanxi Province,China
  • Received:2023-03-14 Revised:2023-05-05 Online:2024-04-25 Published:2024-05-14

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摘要： 目的: 探讨阿托伐他汀对大鼠牙槽骨缺损的治疗作用,并观察对Wnt/β-catenin信号通路的影响。方法: 30只大鼠随机分为正常组（N组）、模型组（M组）与阿托伐他汀给药组（ATV组）,除N组外,其余大鼠均在牙槽骨处制造缺口,构建牙槽骨缺损模型。建模成功后,ATV组灌胃20 mg/kg阿托伐他汀混悬液,N组和M组灌胃等量羧甲基纤维素钠溶液,连续给药21天。末次给药后,尾静脉采血,检测血清骨保护素（OPG）、碱性磷酸酶（ALP）和骨钙素（BPG）浓度。H-E染色观察上颌骨缺损区组织变化情况,并进行Lane Sandhu评分。以抗酒石酸酸性磷酸酶（TRAP）染色检测缺损区破骨细胞数目,实时荧光定量PCR（RT-qPCR）与蛋白免疫印迹（WB）法检测缺损区分泌型糖蛋白（Wnt）、β连锁蛋白（β-catenin）和RUNT相关转录因子2（Runx2）mRNA及蛋白表达量。采用SPSS 23.0软件包对数据进行统计学分析。结果: 与N组相比,M组OPG、ALP和BGP浓度与Lane Sandhu评分降低,破骨细胞数增多。与M组相比,ATV组OPG、ALP和BGP浓度与Lane Sandhu评分上升,破骨细胞数减少。H-E染色可见, N组上颌骨缺损区的骨形成量较多,M组缺损区骨组织较少,ATV组缺损区骨组织量增加。与N组相比,M组上颌骨缺损区Wnt、β-catenin和Runx2 mRNA和蛋白表达量降低。与M组相比,ATV组上颌骨缺损区的Wnt、β-catenin和Runx2 mRNA和蛋白表达量增加。结论: 阿托伐他汀可促进牙槽骨缺损模型大鼠牙槽骨缺损愈合,加快缺损处骨重建,该作用可能与Wnt/β-catenin信号通路激活有关。

关键词: 阿托伐他汀, 牙槽骨缺损, 破骨细胞, β连锁蛋白, RUNT相关转录因子2

Abstract: PURPOSE: To investigate the therapeutic effect of atorvastatin on alveolar bone defect model in rats, and to observe the effect of atorvastatin on Wnt/β-catenin. METHODS: Thirty rats were randomly divided into normal group (group N), model group (group M) and atorvastatin administration group (group ATV). Except group N, bone defects were made in other rats' alveolar bone to construct alveolar bone defect model. After successful modeling, 20 mg/kg atorvastatin suspension was administered by gavage in group ATV, and the same amount of sodium carboxymethyl cellulose solution was administered by gavage in group N and group M for twenty-one days. After the last administration, tail vein blood was collected to detect the concentrations of serum osteoprotegerin (OPG), alkaline phosphatase (ALP) and osteocalcin (BPG). H-E staining was used to observe the pathological changes of maxillary defect area, and lane Sandhu score was performed. Tartrate resistant acid phosphatase(TRAP) staining was used to detect the number of osteoclasts in the defect area. Real time fluorescence quantitative PCR(RT-qPCR) and Western blot(WB) were used to detect Wnt, β-catenin and Runx2 mRNA protein expression. Statistical analysis was performed with SPSS 23.0 software package. RESULTS: Compared with group N, the concentrations of OPG, ALP, BGP and Lane Sandhu score in group M decreased, and the number of osteoclasts increased. Compared with group M, the concentrations of OPG, ALP and BGP and lane Sandhu score in group ATV increased, and the number of osteoclasts decreased. After H-E staining, the amount of bone formation in maxillary defect area in group N was more,there was fewer bone tissues in the defect area in group M, the amount of bone tissues in the defect area increased in group ATV. Compared with group N, Wnt, β-catenin and Runx2 mRNA protein decreased. Compared with group M, Wnt, β-catenin and Runx2 mRNA protein expression increased. CONCLUSIONS: Atorvastatin can promote the healing of alveolar bone defect and accelerate bone reconstruction in rat models. This effect may be related to the activation of Wnt/β-catenin signaling pathway.

Key words: Atorvastatin, Alveolar bone defect, Osteoclasts, β-catenin, Runx2

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