80例牙周炎患者牙龈组织中乙型细环病毒和EB病毒载量分析

doi:10.19439/j.sjos.2022.06.012

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (6): 632-637.doi: 10.19439/j.sjos.2022.06.012

80例牙周炎患者牙龈组织中乙型细环病毒和EB病毒载量分析

于阗^1,2, 张羽², 潘少坤³, 杨彬¹, 冯希平²

1.上海市口腔医院口腔预防科,上海市颅颌面发育与疾病重点实验室,上海 200001;
2.上海交通大学医学院附属第九人民医院口腔预防科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011;
3.上海市重大传染病和生物安全研究院,上海 200032

收稿日期:2021-12-27 修回日期:2022-05-01 发布日期:2022-12-29
通讯作者: 冯希平,E-mail: fxiping1808@qq.com
作者简介:于阗（1989-）,男,硕士,住院医师,E-mail: zzyutian@163.com
基金资助:
国家自然科学基金（81800967,81470737）; 上海市卫生健康委员会科研项目（202140265）; 上海市口腔医院口腔病防治院临床研究创新团队建设项目（SSDC-2020-CXTD-B01）

Prevalence of torque teno mini virus (TTMV) and epstein-barr virus (EBV) in gingival tissues in 80 patients with periodontitis

YU Tian¹, ZHANG Yu², PAN Shao-kun³, YANG Bin¹, FENG Xi-ping²

1. Department of Preventive Dentistry, Shanghai Stomatological Hospital; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University. Shanghai 200001;
2. Department of Preventive Dentistry,Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011;
3. Shanghai Institute of Infectious Disease and Biosecurity, Fudan University. Shanghai 200032, China

Received:2021-12-27 Revised:2022-05-01 Published:2022-12-29

摘要/Abstract

摘要： 目的: 研究80例牙周炎患者牙龈组织中乙型细环病毒（TTMV）和EB病毒（EBV）检出率和病毒载量。方法: 收集80例牙周炎和40例牙周健康受试者的牙龈组织样品,运用巢式PCR检测牙龈组织内EBV和TTMV-222病毒的检出率,运用实时荧光定量PCR检测牙龈组织中2种病毒的载量。采用SPSS 16.0软件包对数据进行统计学分析。结果: 牙周炎组的EBV和TTMV-222病毒检出率和病毒载量显著高于牙周健康组（P<0.05）,且TTMV-222病毒在EBV阳性人群的检出率高于EBV阴性人群（P<0.01）,EBV和TTMV-222在牙龈组织内的病毒载量呈正相关（P<0.01）。结论: TTMV感染以及EBV和TTMV的共感染可能与牙周病相关,但2种病毒相互作用的相关机制需要进一步研究。

关键词: 牙周炎, 乙型细环病毒, EB病毒

Abstract: PURPOSE: To study the prevalence of torque teno mini virus (TTMV) and epstein-barr virus(EBV) in patients with periodontitis. METHODS: Gingival tissue samples were collected from 80 patients with periodontitis and 40 periodontal healthy volunteers. The presence of EBV and TTMV-222 were detected by nested PCR, and the virus loads were detected by real-time PCR. Statistical analysis was performed by SPSS 16.0 software package. RESULTS: The detection rates and virus loads of EBV and TTMV-222 in periodontitis group were significantly higher than those in periodontal health group (P<0.05), and the detection rate of TTMV-222 in EBV positive group was significantly higher than that in EBV negative group (P<0.01). There was a positive correlation between EBV and TTMV-222 in gingival tissues(P<0.01). CONCLUSIONS: TTMV infection and co-infection of EBV and TTMV may be related to periodontal disease, but the pathogenic mechanism of the interaction between the two viruses needs further studies.

Key words: Periodontitis, Torque teno mini virus, Epstein-barr virus

中图分类号:

R788

于阗, 张羽, 潘少坤, 杨彬, 冯希平. 80例牙周炎患者牙龈组织中乙型细环病毒和EB病毒载量分析[J]. 上海口腔医学, 2022, 31(6): 632-637.

YU Tian, ZHANG Yu, PAN Shao-kun, YANG Bin, FENG Xi-ping. Prevalence of torque teno mini virus (TTMV) and epstein-barr virus (EBV) in gingival tissues in 80 patients with periodontitis[J]. Shanghai Journal of Stomatology, 2022, 31(6): 632-637.

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80例牙周炎患者牙龈组织中乙型细环病毒和EB病毒载量分析

Prevalence of torque teno mini virus (TTMV) and epstein-barr virus (EBV) in gingival tissues in 80 patients with periodontitis

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