同期神经化增强髂骨瓣术后骨髓间充质干细胞的活性

doi:10.19439/j.sjos.2020.06.001

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (6): 561-566.doi: 10.19439/j.sjos.2020.06.001

同期神经化增强髂骨瓣术后骨髓间充质干细胞的活性

王旭东^*, 张成瑶^*, 张士剑, 史敬存, 王磊

上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2020-01-31 修回日期:2020-03-19 出版日期:2020-12-25 发布日期:2021-01-08
通讯作者: 王磊,E-mail：wangleizyh@aliyun.com
作者简介:王旭东（1994-）,女,硕士研究生,E-mail：694860480@qq.com;张成瑶（1987-）,女,副主任医师,E-mail：cczhangcy@163.com。^*并列第一作者
基金资助:
国家自然科学基金（81771046、81970907、81270015）; 上海市人才发展资金资助项目（2018042）; 上海第九人民医院“临加”项目（JYLJ201817）

Simultaneous innervation revitalizes bone marrow mesenchymal stem cells from postoperative iliac grafts

WANG Xu-dong, ZHANG Cheng-yao, ZHANG Shi-jian, SHI Jing-cun, WANG Lei

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2020-01-31 Revised:2020-03-19 Online:2020-12-25 Published:2021-01-08

摘要/Abstract

摘要： 目的： 比较神经化髂骨瓣和传统髂骨瓣重建下颌骨缺损术后,移植骨块骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMMSCs）的干细胞活性。方法： 游离血管化髂骨瓣移植修复下颌骨缺损术后半年,从移植骨骨髓体外分离、培养BMMSCs,通过集落形成观察、Brdu摄入实验、群体倍增时间、体外成骨茜素红染色法、裸鼠皮下成骨法检测BMMSCs增殖、自我更新及成骨分化能力。采用SPSS 24.0软件包对数据进行统计学分析。结果： 神经化髂骨移植术后分离、培养的BMMSCs的集落形成、增殖、群体倍增时间、体外及体内成骨分化能力均显著高于非神经化组（P<0.05）。结论： 神经化髂骨瓣重建下颌骨缺损可以增强BMMSCs的自我更新、增殖、成骨分化等潜能,有助于维持移植骨的术后内环境稳定,减少术后移植骨吸收。

关键词: 骨髓间充质干细胞, 神经化, 骨移植, 成骨分化

Abstract: PURPOSE: To compare the osteogenic potential of bone marrow mesenchymal stem cells (BMMSCs) from innervated iliac graft bone flap and traditional one for reconstructing mandibular defects. METHODS: Graft bone marrow samples were harvested 1 year after free vascularized iliac reconstruction of mandibular defects, with or without innervation through simultaneous nerve anastomosis. BMMSCs were isolated and cultured in vitro. Colony forming units-fibrosis observation, Brdu incorporation assay, population doubling, Alizarin red staining for in vitro calcified nodule formation and in vivo assay of subcutaneous osteogenesis in nude mice were used to detect BMMSCs proliferation, self-renewal and osteogenic differentiation capabilities, respectively. SPSS 24.0 software package was used for statistical analysis. RESULTS: Colony formation, proliferation, population doubling and osteogenic differentiation abilities of BMMSCs from innervated group were significantly higher than those from non-innervated group（P<0.05）. CONCLUSIONS: Simultaneous innervation of free vascularized iliac during reconstruction of mandibular defects may maintain self-renewal and osteogenic differentiation potentials of BMMSCs in graft bones, thereby maintaining bone homeostasis and reducing postoperative graft bone resorption.

Key words: Bone marrow mesenchymal stem cells, Innervation, Bone graft, Osteogenic differentiation

中图分类号:

R739.8

王旭东, 张成瑶, 张士剑, 史敬存, 王磊. 同期神经化增强髂骨瓣术后骨髓间充质干细胞的活性[J]. 上海口腔医学, 2020, 29(6): 561-566.

WANG Xu-dong, ZHANG Cheng-yao, ZHANG Shi-jian, SHI Jing-cun, WANG Lei. Simultaneous innervation revitalizes bone marrow mesenchymal stem cells from postoperative iliac grafts[J]. Shanghai Journal of Stomatology, 2020, 29(6): 561-566.

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同期神经化增强髂骨瓣术后骨髓间充质干细胞的活性

Simultaneous innervation revitalizes bone marrow mesenchymal stem cells from postoperative iliac grafts

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