氟化钠对人牙周膜细胞增殖及矿化能力的影响

doi:10.19439/j.sjos.2017.01.010

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (1): 48-53.doi: 10.19439/j.sjos.2017.01.010

氟化钠对人牙周膜细胞增殖及矿化能力的影响

李坤芩^{1, 2}, 徐乐^{1, 2}, 黄圣运³, 张东升^{1, 3}

1.山东大学口腔医学院,山东济南 250012;
2.山东省口腔组织再生重点实验室,山东济南 250012;
3.山东大学附属省立医院口腔科,山东济南 250021

收稿日期:2016-07-05 修回日期:2016-09-08 出版日期:2017-02-25 发布日期:2017-03-20
通讯作者: 张东升,E-mail: ds63zhang@sdu.edu.cn
作者简介:李坤芩（1991-）,女,硕士,E-mail：xingqin9510@163.com

Effect of NaF on proliferation and mineralization of human periodontal ligament cells

LI kun-qin^{1, 2}, XU Le^{1, 2}, HUANG Sheng-yun³, ZHANG dong-sheng^{1, 3}

1.School of Stomatology of Shandong University. Jinan 250012;
2.Shandong Provincial Key Laboratory of Oral Tissue Regeneration. Jinan 250012;
3.Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong University. Jinan 250021, Shandong Province, China

Received:2016-07-05 Revised:2016-09-08 Online:2017-02-25 Published:2017-03-20

摘要/Abstract

摘要： 目的观察氟化钠对体外培养的人牙周膜细胞增殖及矿化能力的影响,为氟添加入牙周组织工程药物中的应用提供依据。方法原代培养并鉴定人牙周膜细胞,应用CCK8检测不同浓度NaF对hPDLCs增殖的影响,并筛选出4个浓度用于矿化实验。矿化条件下,将0、 1×10^-5、 5×10^-4 和1×10^-3 mol/L的NaF作用hPDLCs后,通过碱性磷酸酶（ALP）染色、茜素红染色和实时荧光定量PCR检测矿化能力及成骨相关基因的表达。采用 SPSS20.0 软件包对数据进行单因素方差分析。结果 5×10^-5、1×10^-4、 5×10^-4 mol/L的NaF均能促进hPDLCs 增殖,且以5×10^-4 mol/L效果最佳（P<0.05）。而1×10^-5 mol/L的NaF碱性磷酸酶染色阳性面积最大、茜素红染色矿化结节数量最多（P<0.05）。RT-PCR结果根据时间、指标变化程度较大。结论 5×10^-5、1×10^-4、5×10^-4 mol/L的NaF能促进hPDLCs 的增殖能力, 1×10^-5 mol/L的NaF能提高hPDLCs的碱性磷酸酶活性及钙结节形成。

关键词: 氟, 牙周膜细胞, 矿化, 牙周炎

Abstract: PURPOSE: To investigate the effect of NaF on proliferation and mineralization of human periodontal ligament cells (hPDLCs). METHODS: hPDLCs were isolated and characterized. The proliferation of hPDLCs treated with different concentration of NaF was tested by CCK-8. Four moderate concentrations were chosen for subsequent experiments. The mineralization was investigated using ALP activity assay, Alizarin red S staining and quantitative real-time polymerase chain reaction(RT-PCR). The data were statistically analyzed with SPSS20.0 software package. RESULTS: Immunohistochemistry showed that the isolated cells were hPDLCs. 5×10^-5, 1×10^-4 and 5×10^-4 mol/L NaF had pro-proliferation effects while 5×10^-4 mol/L resulted in the best effect (P<0.05). ALP activity and calcium content was significantly enhanced by 1×10^-5 mol/L NaF with osteogenic inductive medium (P<0.05). Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. CONCLUSIONS: 5×10^-5, 1×10^-4, 5×10^-4 mol/L NaF can stimulate proliferation in hPDLCs, 1×10^-5 mol/L NaF can enhance ALP activity and calcium content formation of hPDLCs.

Key words: Fluoride, Periodontal ligament cells, Mineralization, Periodontitis

中图分类号:

R781.4

李坤芩, 徐乐, 黄圣运, 张东升. 氟化钠对人牙周膜细胞增殖及矿化能力的影响[J]. 上海口腔医学, 2017, 26(1): 48-53.

LI kun-qin, XU Le, HUANG Sheng-yun, ZHANG dong-sheng. Effect of NaF on proliferation and mineralization of human periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2017, 26(1): 48-53.

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氟化钠对人牙周膜细胞增殖及矿化能力的影响

Effect of NaF on proliferation and mineralization of human periodontal ligament cells

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