LIM矿化蛋白1在大鼠牙髓损伤修复中的时空表达

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 157-161.

LIM矿化蛋白1在大鼠牙髓损伤修复中的时空表达

方平娟^1*，潘乙怀^1*，孙仁义²，赵瑜^1*，孙瑜¹

1.温州医科大学附属口腔医院牙体牙髓病科,
2.口腔颌面外科,浙江温州 325027

收稿日期:2015-05-26 修回日期:2015-09-16 出版日期:2016-03-25 发布日期:2016-05-06
作者简介:方平娟(1983-),女,硕士,主治医师,E-mail: fangpingjuan@wzmc.edu.cn 孙仁义,Tel:0577-88063033,E-mail: sunrydoctor@sina.com
基金资助:
温州市科技局项目(Y20130251)

Temporal and spacial expression of LIM mineralization protein 1 during rat pulp injury and reparation

FANG Ping-juan¹, PAN Yi-huai¹, SUN Ren-yi², ZHAO Yu¹ , SUN Yu¹

1. Department of Endodontics,
2.Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital, Wenzhou Medical University. Wenzhou 325027, Zhejiang Province, China

Received:2015-05-26 Revised:2015-09-16 Online:2016-03-25 Published:2016-05-06

摘要/Abstract

摘要： 目的:探讨LIM矿化蛋白1(LIM mineralization protein 1, LMP-1)在大鼠磨牙牙髓损伤修复中的表达。方法:建立大鼠磨牙牙髓损伤修复动物模型,用免疫组织化学染色方法观察LMP-1在大鼠牙髓损伤修复中的表达,并用Image-Pro Plus 6.0图像分析软件及单因素方差分析比较各组间的差异。结果:在正常大鼠牙髓组织中LMP-1表达阴性;在大鼠牙髓损伤后1 d,LMP-1表达于成牙本质细胞及部分牙髓成纤维细胞;术后3 d,LMP-1表达于坏死牙髓下方的细胞增殖层;术后7 d,LMP-1显著表达于增殖活跃的牙髓细胞及部分成牙本质细胞中。结论:LMP-1在牙髓损伤修复过程中呈时空特异性表达,可能参与成牙本质细胞及牙髓细胞的增殖、分化及修复性牙本质的形成。

关键词: LIM矿化蛋白1, 牙髓损伤修复, 时空表达

Abstract: PURPOSE: To investigate the expression and localization of LIM mineralization protein 1(LMP-1) during rat pulp injury and reparation. METHODS: Dental pulp injury models were established in maxillary first molars on one side of 12 Wistar rats, the isonym healthy teeth on the opposite side were used as the controls. Immunohistochemistry technique was used to observe the expression of LMP-1 at 1, 3 and 7 day after pulp injury. The results of staining were analyzed by Image-Pro Plus 6.0 and SPSS 17.0 software package. RESULTS: LMP-1 was not detected in normal rat dental pulp. Positive staining of LMP-1 was found in odontoblasts and some dental pulp cells at 1 day after pulp injury. The expression of LMP-1 was converged at cell proliferation zone under the injury site at 3 and 7 day after injury. CONCLUSIONS: LMP-1 might play a role in the dental pulp cell proliferation and differentiation and reparative dentin formation during pulp injury and reparation.

Key words: LIM mineralization protein 1, Dental pulp injury, Temporal and spacial expression

中图分类号:

R781.31

方平娟，潘乙怀，孙仁义，赵瑜，孙瑜. LIM矿化蛋白1在大鼠牙髓损伤修复中的时空表达[J]. 上海口腔医学, 2016, 25(2): 157-161.

FANG Ping-juan, PAN Yi-huai, SUN Ren-yi, ZHAO Yu , SUN Yu. Temporal and spacial expression of LIM mineralization protein 1 during rat pulp injury and reparation[J]. Shanghai Journal of Stomatology, 2016, 25(2): 157-161.

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