牵张应变诱导人牙周膜细胞晚期凋亡的实验研究

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 404-409.

牵张应变诱导人牙周膜细胞晚期凋亡的实验研究

郝轶, 李晶, 马佳音, 李隽, 胥春

上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,上海市口腔医学重点实验室,上海 200011

收稿日期:2015-01-20 出版日期:2015-08-20 发布日期:2015-09-10
作者简介:郝轶（1980-）,女,博士,主治医师,
基金资助:
国家自然科学基金（31470903）

Cylic stretching force-induced late apoptosis in human periodontal ligament cells

HAO Yi, LI Jing, MA Jia-yin, LI Jun, XU Chun

Department of Prosthodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2015-01-20 Online:2015-08-20 Published:2015-09-10
Supported by:
Supported by National Natural Science Foundation of China (31470903) and Shanghai Pujiang Talent Plan (13PJD021)

摘要/Abstract

摘要： 目的研究机械牵张应变对人牙周膜细胞（human periodontal ligament cells,HPDLCs）晚期凋亡的影响。方法体外培养HPDLCs,利用动态机械应变细胞加载装置对HPDLCs加载1%、10%和20%的动态牵张应变6、12、24和48 h,通过流式细胞仪和激光扫描共聚焦显微镜对经过TUNEL染色的细胞进行晚期凋亡检测。采用SPSS 11.0软件包对数据进行统计学分析。结果HPDLCs加载后,呈现栅栏状相互平行排列趋势,排列方向垂直于牵张力方向。各应变组细胞的晚期凋亡率在加载24 h时达到该应变组的峰值,且具有一定时间和应变依赖性。结论通过动态机械应变细胞加载装置对人牙周膜细胞的牵张加载,发现牵张应变可以诱导细胞发生晚期凋亡,具有时间和应变值依赖性,24 h时晚期凋亡率达到峰值。

关键词: 牵张应变, 晚期凋亡, 人牙周膜细胞

Abstract: PURPOSE: To investigate the effect of stretching force on late apoptosis of human periodontal ligament cells. METHODS: HPDLCs were cultured in vitro, and cells were stretched by dynamic mechanical strain 1%, 10% and 20% for 6, 12, 24 and 48 h. Then late apoptosis were detected through TUNEL method binding by flow cytometry and confocal laser scanning microscopy. The data was analyzed with SPSS 11.0 software package. RESULTS: HPDCLs inclined parallel to each other and aligned their long axis perpendicular to the stretching force vector. The number of apoptotic cells increased significantly in a time-and force-dependent manner in response to stretching strain within 24 h. CONCLUSIONS: It is found that stretching force can induce apoptosis in HPDLC and show time- and stress-dependent manner with a tendency of increase in 24 h.

Key words: Stretching force, Late apoptosis, HPDLCsShanghai J Stomatol, 2015, 24(4):404-409.

中图分类号:

R783.5

郝轶, 李晶, 马佳音, 李隽, 胥春. 牵张应变诱导人牙周膜细胞晚期凋亡的实验研究[J]. 上海口腔医学, 2015, 24(4): 404-409.

HAO Yi, LI Jing, MA Jia-yin, LI Jun, XU Chun. Cylic stretching force-induced late apoptosis in human periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2015, 24(4): 404-409.

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