牛蒡苷元对人口腔鳞癌裸鼠移植瘤生长及VEGF蛋白表达的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 400-403.

牛蒡苷元对人口腔鳞癌裸鼠移植瘤生长及VEGF蛋白表达的影响

浦光瑞¹, 刘法昱², 王博¹

1.大连医科大学附属第一医院口腔科,辽宁大连 116011; 2.中国医科大学附属口腔医院口腔颌面外科,辽宁沈阳 110001

收稿日期:2015-03-31 出版日期:2015-08-20 发布日期:2015-09-10
通讯作者: 王博,E-mail：wwangbo_306@163.com E-mail:wwangbo_306@163.com
作者简介:浦光瑞（1982-）,女,硕士研究生,主治医师,E-mail：dl_pugr@126.com
基金资助:
国家自然科学基金（81102058）

Suppression of VEGF protein expression by arctigenin in oral squamous cell carcinoma

PU Guang-rui¹, LIU Fa-yu², WANG Bo¹

1.Department of Dentistry, The First Affiliated Hospital of Dalian Medical University. Dalian 116011; 2. Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of China Medical University. Shenyang 110001, Liaoning Province, China

Received:2015-03-31 Online:2015-08-20 Published:2015-09-10

摘要/Abstract

摘要： 目的观察牛蒡苷元对口腔鳞癌的抑制作用,并探讨其可能的作用机制。方法应用免疫组织化学法检测32例口腔鳞癌及20例癌旁组织中VEGF蛋白的表达情况;制备人口腔鳞癌细胞株HSC-3裸鼠移植瘤模型,牛蒡苷元处理后,检测移植瘤生长情况及移植瘤组织中VEGF蛋白的表达情况。采用SPSS 16.0软件包对数据进行单因素方差分析。结果与癌旁组织相比,口腔鳞癌组织中VEGF蛋白的免疫组织化学染色的总积分显著增高（P<0.001）;经牛蒡苷元处理后,人口腔鳞癌裸鼠移植瘤体积增长趋势显著放缓（P<0.05）,观察终点移植瘤组织重量显著下降（P<0.05）,且牛蒡苷元高剂量组较牛蒡苷元低剂量组更为明显（P<0.05）;与模型组相比,牛蒡苷元处理组VEGF蛋白染色的吸光度值显著下降（P<0.05）,高剂量组较低剂量组下降更加显著（P<0.05）。结论牛蒡苷元可以剂量依赖性方式抑制口腔鳞癌增殖,该作用可能与抑制VEGF蛋白的表达有关。

关键词: 牛蒡苷元, 口腔鳞癌, 血管内皮细胞生长因子

Abstract: PURPOSE: To observe arctigenin’s inhibitory effect on oral squamous cell carcinoma, and explore the possible mechanism. METHODS: The expression of VEGF in 32 cases of oral squamous cell cancer and 20 adjacent tissue specimen were detected with immunohistochemistry. Human nude mouse transplantation tumor model of oral squamous cell cancer was prepared with HSC-3 cells line. Transplanted tumor growth and VEGF expression in transplanted tumor tissues were assayed after treatment with arctigenin. One-way ANOVA was used for comparison between groups with SPSS 16.0 software package. RESULTS: Compared with the adjacent tissue, immunohistochemical staining score of VEGF was significantly higher (P<0.01) in oral squamous cell carcinoma tissues. After treatment with arctigenin, the growth of oral squamous cell transplanted tumors in nude mouse was inhibited (P<0.05), and decreased weight in end point of observation was noted (P<0.05). There were significant differences between high dose group and low dose group (P<0.05). Compared with the nude mouse model group, the optical density of VEGF staining was significantly lower in arctigenin group (P<0.05). There were significant differences between high dose group and low dose group (P<0.05). CONCLUSIONS: Arctigenin can dose-dependently inhibit the growth of oral squamous cell carcinomas, and this effect may be related to down regulation of VEGF expression.

Key words: Arctigenin, Oral squamous cell carcinoma, Vascular endothelial growth factor Shanghai J Stomatol, 2015, 24(4):400-403.

中图分类号:

R783.5

浦光瑞, 刘法昱, 王博. 牛蒡苷元对人口腔鳞癌裸鼠移植瘤生长及VEGF蛋白表达的影响[J]. 上海口腔医学, 2015, 24(4): 400-403.

PU Guang-rui, LIU Fa-yu, WANG Bo. Suppression of VEGF protein expression by arctigenin in oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2015, 24(4): 400-403.

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