ffh基因沉默对变形链球菌耐氟菌株耐酸性的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 395-399.

ffh基因沉默对变形链球菌耐氟菌株耐酸性的影响

李振玲, 张志民, 李朋莲, 张桐菲, 李天博, 刘富萍

吉林大学口腔医院牙体牙髓病科,吉林长春 130021

收稿日期:2014-08-25 出版日期:2015-08-20 发布日期:2015-09-10
作者简介:李振玲（1987-）,女,在读硕士研究生,
基金资助:
国家自然科学基金 (3A411X463431)

Evaluation of the effect for ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans

LI Zhen-ling, ZHANG Zhi-min, LI Peng-lian, ZHANG Tong-fei, LI Tian-bo, LIU Fu-ping

Department of Endodontics, Hospital of Stomatology, Jilin University. Changchun 130021, Jilin Province, China

Received:2014-08-25 Online:2015-08-20 Published:2015-09-10
Supported by:
Supported by National Natural Science Foundation of China (3A411X463431)

摘要/Abstract

摘要： 目的分析ffh基因沉默对变形链球菌耐氟菌株体外耐酸能力的影响。方法利用电穿孔法,将siRNA转入UA159-FR,使其与ffh基因靶向位点结合,筛选出含ffh基因沉默的变形链球菌耐氟菌株,与UA159-FR的标准菌液分别在不同pH值（以0.5为间隔,pH为3.5~7.5）的BHI液体培养基中37℃微需氧（95%N₂、5%CO₂）培养24 h,离心,采用pH计测定培养物上清的终末pH值;5 mL生理盐水稀释细菌沉淀,用紫外分光光度计测定600 nm处的吸光度,采用SPSS 17.0软件包对所得数据进行统计学分析。结果原菌株UA159-FR与ffh基因沉默的变形链球菌UA159-FR相比,ΔpH差异显著（P<0.05）,且前者高于后者。两者比较pH=3.5~5.0时,OD₆₀₀有显著差异（P<0.01）;pH=5.5~7.5时,OD₆₀₀有显著差异（P<0.05）,且两者生长趋势相似。结论ffh基因沉默对变形链球菌耐氟菌株的耐酸性有一定影响。

关键词: ffh, 基因沉默, 变形链球菌耐氟菌株, 耐酸性

Abstract: PURPOSE: To analyze the effect of ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans in vitro. METHODS: By using electroporation, UA159-FR was transformed and combined with targeted site of ffh gene sequence, and the best piece of siRNA for fluoride resistant Streptococcus mutans was screened. In different values of pH of BHI, they were cultured for 24 hours with UA159-FR respectively, and then centrifugated to determine the pH and OD_600. SPSS17.0 software package was used to analyze the data. RESULTS: The aciduricity of UA159-FR had significant differences compared with ffh gene silencing for UA159-FR in ΔpH (P<0.05), and the former was higher than the latter. At pH=3.5~5.0, P<0.01; at pH=5.5~7.5, P<0.05. Significant differences were noted in OD₆₀₀ and their growth tendency were similar. CONCLUSIONS: The aciduricity of fluoride resistant Streptococcus mutans has significant effect when the ffh gene is silenced.

Key words: ffh, Gene silencing, Fluoride resistant Streptococcus mutans, Aciduricity Shanghai J Stomatol,2015, ,24(4):395?399.

中图分类号:

R783.6

李振玲, 张志民, 李朋莲, 张桐菲, 李天博, 刘富萍. ffh基因沉默对变形链球菌耐氟菌株耐酸性的影响[J]. 上海口腔医学, 2015, 24(4): 395-399.

LI Zhen-ling, ZHANG Zhi-min, LI Peng-lian, ZHANG Tong-fei, LI Tian-bo, LIU Fu-ping. Evaluation of the effect for ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans[J]. Shanghai Journal of Stomatology, 2015, 24(4): 395-399.

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