氟对纯钛表面变形链球菌和血链球菌黏附的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (2): 141-146.

氟对纯钛表面变形链球菌和血链球菌黏附的影响

王珏¹,乔广艳¹,沈庆平¹,顾凯凯²

1.上海市口腔病防治院修复科,上海 200001;
2.同济大学口腔医学院修复科,上海 200072

收稿日期:2012-12-10 出版日期:2015-04-20 发布日期:2015-07-24
通讯作者: 乔广艳,Tel：021-63601035,E-mail:g_y_qiao@126.com
作者简介:王珏（1974-）,女,硕士,副主任医师,E-mail:13818983828@163.com
基金资助:
上海市卫计委青年课题（20114Y053）; 上海市自然科学基金(12ZR1427200)

The effect of fluoride on adhesion of Streptococcus mutans and Streptococcus sanguis on the dental pure titanium surface

WANG Jue¹,QIAO Guang-yan¹,SHEN Qing-ping¹,GU Kai-kai²

1.Department of Prosthodontics, Shanghai Stomatology Disease Center. Shanghai 200001;
2. Department of Prosthodontics, School of Stomatology, Tongji University. Shanghai 200072, China

Received:2012-12-10 Online:2015-04-20 Published:2015-07-24
Supported by:
Youth Fund of Shanghai Health and Family Planning Commission (20114Y053)and Natural Science Foundation of Shanghai Municipality (12ZR1427200)

摘要/Abstract

摘要： 目的研究氟对纯钛表面变形链球菌和血链球菌黏附的影响。方法采用四唑盐比色法检测经不同浓度氟化钠人工唾液浸泡24 h后变形链球菌和血链球菌在纯钛表面的黏附情况,并用原子力显微镜分析变形链球菌和血链球菌黏附后纯钛表面的粗糙度及表面形貌。采用SPSS 11.0软件包对数据进行统计学分析。结果经不同浓度氟化钠人工唾液浸泡后,变形链球菌和血链球菌在纯钛表面黏附的OD值随着氟化钠浓度的升高越来越大：0 g/L < 0.5 g/L < 1.0 g/L < 2.0g/L (P<0.05)。此外,变形链球菌和血链球菌黏附后,纯钛表面的粗糙度Ra值随着氟化钠浓度的升高也越来越大：0 g/L < 0.5 g/L < 1.0 g/L < 2.0g/L (P<0.05);纯钛的表面形貌有明显差异,无论是经变形链球菌还是经血链球菌黏附后,随着氟化钠浓度的升高,纯钛表面均变得越来越粗糙、不平整。结论经不同浓度氟化钠人工唾液浸泡后,变形链球菌和血链球菌在纯钛表面的黏附、黏附后粗糙度及表面形貌结果一致,提示氟化钠加剧了纯钛的腐蚀,使得纯钛表面的粗糙度增加,进而促进了变形链球菌和血链球菌在纯钛表面的黏附。

关键词: 氟离子, 牙科纯钛, 细菌黏附性能, 表面粗糙度

Abstract: PURPOSE: This study was to investigate the effect of fluoride on adhesion of Streptococcus mutans and Streptococcus sanguis on the dental pure titanium surface. METHODS: The adhesion of Streptococcus mutans and Streptococcus sanguis on the dental pure titanium surface were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after soaked in artificial saliva containing different concentrations of sodium fluoride for 24 h. Then the surface roughness and surface morphology of the pure titanium were analyzed by atomic force microscope after bacterial adhesion experiments. The data was statistically analyzed by analysis of variance(ANOVA) with SPSS11.0 software package. RESULTS: After soaked in artificial saliva containing different concentrations of sodium fluoride for 24 h, the sequence of OD values showed the adhesion of Streptococcus mutans and Streptococcus sanguis on dental pure titanium surface was 0 g/L < 0.5 g/L < 1.0g/L < 2.0 g/L (P<0.05). In addition, the sequence of Ra values showed the surface roughness of pure titanium after bacterial adhesion experiments was 0 g/L < 0.5 g/L < 1.0g/L < 2.0 g/L (P<0.05). And the surface morphology of pure titanium was consistent with the changes of OD value and Ra value. CONCLUSIONS: The results of adhesion of Streptococcus mutans and Streptococcus sanguis, surface roughness and surface morphology reveal that fluoride aggravates the corrosion of pure titanium, increases the surface roughness, and thus promots the adhesion of Streptococcus mutans and Streptococcus sanguis on the titanium surface.

Key words: Fluoride ions, Dental pure titanium, Adhesion of bacteria, Surface roughness

中图分类号:

R783.1

王珏,乔广艳,沈庆平,顾凯凯. 氟对纯钛表面变形链球菌和血链球菌黏附的影响[J]. 上海口腔医学, 2015, 24(2): 141-146.

WANG Jue,QIAO Guang-yan,SHEN Qing-ping,GU Kai-kai. The effect of fluoride on adhesion of Streptococcus mutans and Streptococcus sanguis on the dental pure titanium surface[J]. Shanghai Journal of Stomatology, 2015, 24(2): 141-146.

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