microRNA-125b 在口腔鳞状细胞癌患者血浆中的表达及意义

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (1): 71-75.

microRNA-125b 在口腔鳞状细胞癌患者血浆中的表达及意义

顾文莉¹,叶冬霞²,吴静静¹

1.上海交通大学医学院附属第九人民医院检验科,,上海 200011;
2.上海市口腔医学研究所,上海市口腔医学重点实验室,上海 200011

收稿日期:2014-10-13 出版日期:2015-02-20 发布日期:2015-07-24
通讯作者: 顾文莉,E-mail:guw172@gmail.com
作者简介:顾文莉(1972-),女,博士,副主任技师
基金资助:
国家自然科学基金（81101642, 81202132）

Expression and clinical significance of plasma microRNA-125b level in patients with oral squamous cell carcinoma

GU Wen-li¹,YE Dong-xia²,WU Jing-jing¹

1.Department of Clinical Laboratory, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;
2. Shanghai Research Institute of Stomatology,Shanghai Key Laboratory of Stomatology. Shanghai 200011,China

Received:2014-10-13 Online:2015-02-20 Published:2015-07-24
Supported by:
National Natural Science Foundation of China (81101642, 81202132 )

摘要/Abstract

摘要： 目的探讨microRNA-125b (miR-125b) 在口腔鳞状细胞癌 (OSCC) 患者血浆中的表达及临床意义。方法用茎环法反转录 (RT)-实时荧光PCR法检测85例OSCC患者和46例健康人血浆中miR-125b的表达水平,评估其作为OSCC诊断标志的检测效能。采用SPSS17.0软件包中的单因素方差分析和ROC曲线分析对结果进行统计学处理。结果反转录PCR法可特异性扩增血浆中的miR-125b,与正常对照组相比,OSCC患者组血浆miR-125b表达水平显著上调 (P<0.05);ROC曲线分析显示,血浆miR-125b水平可有效区分OSCC患者和正常人群,其曲线下面积（AUC）达到0.966;当确定最佳截断值为3.46 (fold值) 时,血浆miR-125b对OSCC的诊断敏感度和特异度分别达到89.4% 和93.5%。结论鉴于其良好的诊断效能,血浆miR-125b有望成为具有潜在应用价值的OSCC生物学标志物。

关键词: 口腔鳞状细胞癌, 微RNA-125b, 血浆检测, 生物标志物

Abstract: PURPOSE: To explore the expression and clinical significance of plasma microRNA-125b (miR-125b) in patients with oral squamous cell carcinoma (OSCC). METHODS: By Stem-loop RT real-time PCR based on the SYBR Green, we investigated the expression of plasma miR-125b in 85 OSCC patients and 46 healthy controls, then evaluated its diagnostic performance as OSCC biomarker. Data analysis was performed with SPSS 17.0 software package for one-way ANOVA and receiver operating characteristic (ROC) curve. RESULTS: Stem-loop RT-PCR could specifically amplify miR-125b in plasma. The expression of plasma miR-125b was significantly increased in patients with OSCC compared with healthy controls (P<0.05). ROC results showed that miR-125b could differentiate OSCC from healthy controls(AUC=0.966). As fold change 3.46 was chosen for optimal cutoff value, the diagnostic sensitivity and specificity was 89.4% and 93.5%, respectively. CONCLUSIONS: Due to the satisfactory diagnostic performance, plasma miR-125b can be used as a promising biomarker in OSCC.

Key words: Oral squamous cell carcinoma, miR-125b, Plasma detection, Biomarker

中图分类号:

R739.8

顾文莉,叶冬霞,吴静静. microRNA-125b 在口腔鳞状细胞癌患者血浆中的表达及意义[J]. 上海口腔医学, 2015, 24(1): 71-75.

GU Wen-li,YE Dong-xia,WU Jing-jing. Expression and clinical significance of plasma microRNA-125b level in patients with oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2015, 24(1): 71-75.

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