根尖牙乳头干细胞和人脐静脉血管内皮细胞联合培养对牙髓组织成血管能力的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (5): 547-552.

根尖牙乳头干细胞和人脐静脉血管内皮细胞联合培养对牙髓组织成血管能力的影响

袁长永^{1, 2, 3}, 王鹏来^{1, 2}, 张成飞³, 刘宗响⁴, 罗三莲⁵

1.徐州医学院附属徐州口腔医院种植中心,江苏徐州 221002; 2.徐州医学院口腔医学院,江苏徐州 221004; 3.香港大学牙医学院,香港特别行政区 999077; 4.徐州医学院附属徐州口腔医院牙周病科,江苏徐州 221002; 5.徐州医学院第二附属医院口腔科,江苏徐州 221006

收稿日期:2014-01-06 修回日期:2014-03-13 出版日期:2014-10-20 发布日期:2015-02-04
通讯作者: 王鹏来,Tel：0516-85645181,Fax：0516-85866007,E-mail：wpl0771@163.com
作者简介:袁长永（1983-）,男,主治医师,硕士研究生,E-mail：cyyuan@hku.hk
基金资助:
徐州市科技计划项目（XM13B094）; 徐州市医学青年后备人才基金（2014014）

Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues

YUAN Chang-yong^{1, 2, 3}, WANG Peng-lai^{1, 2}, ZHANG Cheng-fei³, LIU Zong-xiang⁴, LUO San-lian⁵

1.Dental Implant Center, Xuzhou Medical College Affiliated Xuzhou Stomatological Hospital. Xuzhou 221002, Jiangsu Province; 2.Xuzhou Medical College School of Stomatology. Xuzhou 221004, Jiangsu Province; 3. Faculty of Dentistry, The University of Hong Kong. Hong Kong 999077; 4. Department of Periodontics, Xuzhou Medical College Affiliated Xuzhou Stomatological Hospital. Xuzhou 221002, Jiangsu Province; 5. Department of Stomatology, Xuzhou Medical College Second Affiliated Hospital. Xuzhou 221006, Jiangsu Province, China

Received:2014-01-06 Revised:2014-03-13 Online:2014-10-20 Published:2015-02-04
Supported by:
; Supported by Science and Technology Planning Project of Xuzhou City (XM13B094) and Foundation for Young Reserve Medical Talents of Xuzhou City (2014014)

摘要/Abstract

摘要： 目的探讨联合培养根尖牙乳头干细胞（stem cells from the apical papilla,SCAPs）和人脐静脉血管内皮细胞（human umbilical vein endothelial cells,HUVECs）对牙髓组织血管形成能力的影响。方法分别使用α-MEM培养基和成骨、成脂、成神经诱导培养基处理SCAPs,采用油红O染色、茜素红染色、βIII-Tubulin免疫荧光染色,分别检测SCAPs的成脂、成骨和神经向分化能力。取单独SCAPs、HUVECs细胞以及联合培养SCAPs和HUVECs细胞进行小管形成实验,分别在3、6、9 h检测各组小管形成的长度、节点数目和结合区面积。采用SPSS16.0软件包对数据进行统计学分析。结果染色结果显示,实验组SCAPs形成了更多的矿化结节、红色脂滴和神经样细胞。小管形成实验结果表明,联合培养组细胞可以更快地形成类血管状结构,差异显著。结论 SCAPs具有成骨、成脂和神经向诱导分化能力,联合培养SCAPs和HUVECs可以加速血管结构的形成。

关键词: 根尖牙乳头干细胞, 人脐静脉血管内皮细胞, 牙髓再生, 血管生成

Abstract: PURPOSE: To observe the effect of coculture of stem cells from the apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs) on the angiogenic potential of dental pulp tissues. METHODS: SCAPs were incubated in osteo/odontogenic, adipogenic, neurogenic induction medium and α-MEM medium, whose multilineage differentiation capacities were confirmed using alizarin red staining, oil red O staining and βⅢ-tubulin immunofluorescent staining. The tubular length, branching points number and junctional areas were detected after 3, 6, 9 h since cells were seeded onto matrigel, and the data was analyzed using SPSS 16.0 software package. RESULTS: SCAPs in the experimental groups were detected having more lipid droplets, mineralization nodules and neuron-like cells. Coculture of SCAPs and HUVECs formed more vessel-like structures in tubular formation assay. CONCLUSIONS: SCAPs are capable of differentiating into fat, bone, and nerve-like cells in vitro. Coculture of SCAPs and HUVECs can enhance the angiogenic potential of dental pulp tissues.

Key words: Stem cells from the apical papilla, Human umbilical vein endothelial cells, Dental pulp regeneration, Angiogenesis

袁长永, 王鹏来, 张成飞, 刘宗响, 罗三莲. 根尖牙乳头干细胞和人脐静脉血管内皮细胞联合培养对牙髓组织成血管能力的影响[J]. 上海口腔医学, 2014, 23(5): 547-552.

YUAN Chang-yong, WANG Peng-lai, ZHANG Cheng-fei, LIU Zong-xiang, LUO San-lian. Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues[J]. Shanghai Journal of Stomatology, 2014, 23(5): 547-552.

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