上海口腔医学

上海口腔医学 ›› 2026, Vol. 35 ›› Issue (1): 29-34.doi: 10.19439/j.sjos.2026.01.005

• 论著 • 上一篇    下一篇

三叶青提取物对OSC-4细胞增殖、凋亡及对NLRP3/PRKD3蛋白水平的影响

杨丹, 雷明辉, 王洁, 姬小婷, 宋凯, 刘彬   

  1. 陕西中医药大学附属医院 口腔科,陕西 咸阳 712000
  • 收稿日期:2024-04-07 修回日期:2025-05-04 出版日期:2026-03-12 发布日期:2026-03-12
  • 通讯作者: 姬小婷,E-mail:qixiaoting1988@163.com
  • 作者简介:杨丹（1985-）,女,硕士,副主任医师,E-mail：ptnb87@sina.com
  • 基金资助:
    国家自然科学基金（81860774、81502340）

Effect of Trifolium repens extract on OSC-4 cell proliferation, apoptosis and NLRP3/PRKD3 protein level

Yang Dan, Lei Minghui, Wang Jie, Ji Xiaoting, Song Kai, Liu Bin   

  1. Department of Stomatology, Affiliated Hospital of Shaanxi University of Chinese Medicine. Xianyang 712000, Shaanxi Province, China
  • Received:2024-04-07 Revised:2025-05-04 Online:2026-03-12 Published:2026-03-12

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摘要： 目的 探讨三叶青提取物对口腔鳞状细胞癌细胞株OSC-4增殖、凋亡、侵袭及NLRP3/PRKD3蛋白水平的影响。方法 将OSC-4细胞随机分为SO组（OSC-4细胞不加入三叶青提取物培养）、SA组（OSC-4细胞加入1 mg/mL三叶青提取物培养）、SB组（OSC-4细胞加入5 mg/mL三叶青提取物培养）和SC组（OSC-4细胞加入10 mg/mL三叶青提取物培养）。采用MTT检测法检测细胞增殖,流式细胞仪检测细胞凋亡,Transwsll小室检测细胞侵袭,Western免疫印迹及PCR分别检测NLRP3/PRKD3表达水平。结果 各组细胞NLRP3、PRKD3 mRNA表达水平比较显示,与SO组相比,SA组、SB组及SC组的表达水平显著降低,降低程度依次为SC组>SB组>SA组（P<0.05）。0 h时,各组细胞增殖抑制率相比无显著差异（P>0.05）;48 h, SA组、SB组和SC组增殖抑制率显著高于SO组（P<0.05）。各组OSC-4细胞中,凋亡率相比差异显著（P<0.001）;SA组、SB组及SC组OSC-4细胞凋亡率显著高于SO组（P<0.05）。各组OSC-4细胞侵袭数目相比,差异有统计学意义（P<0.001）;SA组、SB组及SC组OSC-4细胞侵袭数目显著低于SO组（P<0.05）。各组NLRP3、PRKD3蛋白表达相比,差异有统计学意义（P<0.001）;SA组、SB组及SC组NLRP3、PRKD3蛋白显著低于SO组（P<0.05）。结论 三叶青提取物可抑制OSCC细胞增殖和侵袭,促进其凋亡,其作用机制可能与调控NLRP3/PRKD3表达有关。

关键词: 口腔鳞状细胞癌, 三叶青提取物, NOD样受体热蛋白结构域相关蛋白3, 蛋白激酶D3, 增殖, 凋亡

Abstract: PURPOSE: To investigate the effects of Trifolium repens extract on proliferation, apoptosis, invasion and NLRP3/PRKD3 protein levels of oral squamous cell carcinoma cell line OSC-4. METHODS: The OSC-4 cells were randomly divided into SO group (OSC-4 cells were cultured without adding Trifolium repens extract), SA group (OSC-4 cells were cultured with 1 mg/mL Trifolium repens extract), SB group(OSC-4 cells were cultured with 5 mg/mL Trifolium repens extract), and SC group(OSC-4 cells were cultured with 10 mg/mL Trifolium repens extract). MTT was used to detect cell proliferation, flow cytometry was used to detect cell apoptosis, Transwsll chamber assay was used to evaluate cell invasion, Western blot and PCR were used to detect the expression of NLRP3/PRKD3. RESULTS: Compared with SO group, the expression of NLRP3 and PRKD3 mRNA in SA group, SB group and SC group were significantly reduced, and the degree of reduction was in order of SC group>SB group>SA group(P<0.05). There was no significant difference in the inhibition rate of cell proliferation in each group at 0 h(P>0.05). At 48 h, the proliferation inhibition rate of SA group, SB group, and SC group was significantly higher than that of SO group(P<0.05). There was significant difference in the apoptosis rate of OSC-4 cells in each group (P<0.001). The apoptosis rate of OSC-4 cells in SA group, SB group and SC group was significantly higher than that in SO group(P<0.05). There was significant difference in the number of OSC-4 cell invasion in each group(P<0.001). The number of OSC-4 cell invasion in SA group, SB group and SC group was significantly lower than that in SO group(P<0.05). The differences in the expression of NLRP3 and PRKD3 protein in each group was significant(P<0.001). The NLRP3 and PRKD3 proteins of SA group, SB group and SC group were significantly lower than those of SO group (P<0.05). CONCLUSIONS: Sanye green extract can inhibit OSCC cell proliferation and invasion, promote its apoptosis. Its mechanism of action may be related to the regulation of NLRP3/PRKD3 expression.

Key words: OSCC, Herba trifolia extract, NLRP3, PRKD3, Proliferation, Apoptosis

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