循环牵张力促进周细胞成骨分化及成血管活性的研究

doi:10.19439/j.sjos.2026.01.003

上海口腔医学 ›› 2026, Vol. 35 ›› Issue (1): 13-18.doi: 10.19439/j.sjos.2026.01.003

循环牵张力促进周细胞成骨分化及成血管活性的研究

Chin Daphne Yuchen, 唐昕月, 赵宁, 唐国华

上海交通大学医学院附属第九人民医院口腔正畸科,上海交通大学口腔医学院,国家口腔医学中心, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所, 中国医学科学院口腔颌面再生医学创新单元,上海 200011

收稿日期:2024-11-25 修回日期:2024-12-16 出版日期:2026-03-12 发布日期:2026-03-12
通讯作者: 唐国华,E-mail：drtanggh@163.com
作者简介:Chin Daphne Yuchen（1997-）,女,在读硕士研究生,E-mail: daphne97923@vip.qq.com
基金资助:
国家自然科学基金（82171011）; 上海市自然科学基金（19ZR1429600）; 上海市重中之重研究中心（2022ZZ01017）; 中国医学科学院医学与健康科技创新工程（2019-I2M-5-037）

Cyclic tensile strain promotes osteogenic differentiation and angiogenic activity of mouse pericytes

Chin Daphne Yuchen, Tang Xinyue, Zhao Ning, Tang Guohua

Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences. Shanghai 200011, China

Received:2024-11-25 Revised:2024-12-16 Online:2026-03-12 Published:2026-03-12

摘要/Abstract

摘要： 目的探讨循环牵张力对小鼠周细胞（pericytes,PCs）成骨分化和成血管活性的影响。方法从小鼠脑微血管中分离培养原代PCs,采用流式细胞技术检测其表面抗原。使用Flexcell应力加载系统对细胞施加6%循环拉伸力,将小鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）作为阳性对照。采用碱性磷酸酶（alkaline phosphatase,ALP）和茜素红（alizarin red,AR）染色、Matrigel血管生成实验评价机械应力对PCs成骨分化和成血管活性的影响;qRT-PCR检测成骨及成血管相关基因的表达水平。结果 PCs 为CD29+CD44+CD45-CD31-的间充质干细胞,高表达PDGFRβ。在6%机械应力下,PCs的ALP活性提高1.6倍,AR活性提高2.9倍;BMSCs的ALP活性提高1.5倍,AR活性提高3.9倍。Matrigel血管生成实验显示,机械应力作用下PCs和BMSCs的条件培养基可诱导人脐静脉内皮细胞（human umbilical vein endothelial cells,HUVECs）的成血管活性。qRT-PCR显示,机械应力下PCs表达更高的RUNX2、OCN和IBSP,BMSCs表达更高的VEGF和VEGFR1。结论循环牵张力可促进PCs的成骨分化和成血管活性。

关键词: 周细胞, 成骨分化, 成血管活性, 循环牵张力

Abstract: PURPOSE: To evaluate the effects of cyclic tensile strain on osteogenic differentiation and angiogenic activity of mouse pericytes (PCs). METHODS: Primary mouse brain microvascular PCs were isolated and cultured, and the membranous markers were detected by flow cytometry. Flexcell loading system was used to apply 6% cyclic tensile strain to the cells. Mouse bone marrow mesenchymal stem cells (BMSCs) were used as a positive control. Alkaline phosphatase (ALP) assay, alizarin red (AR) staining and Matrigel angiogenesis test were used to evaluate the effects of mechanical strain on the osteogenic differentiation and angiogenic activity of PCs. The expression levels of osteogenic and angiogenic genes were determined by qRT-PCR. RESULTS: The PCs expressed the characteristic mouse stem cell surface proteins CD29+CD44+CD45-CD31- and highly expressed PDGFRβ. Under 6% cyclic strain, the ALP activity of PCs increased by 1.6-fold and the AR activity by 2.9-fold. Similarly, the ALP and AR activities of BMSCs were increased by 1.5 and 3.9 times, respectively. Matrigel angiogenesis assay showed that the angiogenic activity of human umbilical vein endothelial cells (HUVECs) was induced by conditioned medium from PCs and BMSCs under mechanical strain. qRT-PCR showed that the expressions of RUNX2, OCN and IBSP were higher in PCs under mechanical strain, and the expressions of VEGF and VEGFR1 were higher in BMSCs. CONCLUSIONS: Cyclic tensile strain promotes osteogenic differentiation and angiogenic activity of PCs.

Key words: Pericytes, Osteogenic differentiation, Angiogenesis, Cyclic tension

中图分类号:

R318

Chin Daphne Yuchen, 唐昕月, 赵宁, 唐国华. 循环牵张力促进周细胞成骨分化及成血管活性的研究[J]. 上海口腔医学, 2026, 35(1): 13-18.

Chin Daphne Yuchen, Tang Xinyue, Zhao Ning, Tang Guohua. Cyclic tensile strain promotes osteogenic differentiation and angiogenic activity of mouse pericytes[J]. Shanghai Journal of Stomatology, 2026, 35(1): 13-18.

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循环牵张力促进周细胞成骨分化及成血管活性的研究

Cyclic tensile strain promotes osteogenic differentiation and angiogenic activity of mouse pericytes

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