PERK对人牙周膜细胞成骨分化的调控作用

doi:10.19439/j.sjos.2025.06.002

上海口腔医学 ›› 2025, Vol. 34 ›› Issue (6): 571-576.doi: 10.19439/j.sjos.2025.06.002

PERK对人牙周膜细胞成骨分化的调控作用

李莉芬, 杜嵘, 江龙

上海交通大学医学院附属第九人民医院口腔综合科, 上海交通大学口腔医学院, 国家口腔医学中心, 口腔疾病国家临床医学研究中心, 上海市口腔医学重点实验室, 上海市口腔医学研究所, 上海 200011

收稿日期:2025-03-10 修回日期:2025-04-22 发布日期:2025-12-30
通讯作者: 江龙,E-mail：jianglong25@163.com;杜嵘,E-mail：sumerdr@163.com。^#共同通信作者
作者简介:李莉芬（1986-）,女,博士,E-mail：yuyo2013@126.com
基金资助:
上海交通大学医学院附属第九人民医院“交叉”研究基金项目（JYJC202234）; 上海交通大学医学院附属第九人民医院临床研究助推计划（JYLJ202313）

The regulation of PERK on osteogenic differentiation of human periodontal ligament cells

Li Lifen, Du Rong, Jiang Long

Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Medical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2025-03-10 Revised:2025-04-22 Published:2025-12-30

摘要/Abstract

摘要： 目的:探讨蛋白激酶样内质网激酶（RNA-activated protein kinase-like ER resident kinase,PERK）对人牙周膜细胞（periodontal ligament cells,PDLCs）成骨分化的调控作用。方法:原代培养PDLCs,采用 siRNA 技术沉默PDLCs中 PERK基因的表达,并通过成骨分化诱导培养液进行体外诱导,利用碱性磷酸酶（alkaline phosphatase,ALP）和茜素红染色检测PDLCs成骨分化能力的变化。结果:将PDLCs体外成骨诱导培养后,ALP染色及茜素红染色增强,矿盐沉积增加,同时磷酸化PERK表达增加（P<0.05）。转染si-PERK后,PDLCs的 PERK蛋白表达显著下调（P<0.05）。ALP染色和茜素红染色结果显示,PERK^-/-组ALP染色强度及钙盐沉积显著低于成骨诱导组,同时伴随转录调控因子4（activating transcription factor 4,ATF4）蛋白表达水平下降。结论:PERK能够调控PDLCs的成骨分化。

关键词: 人牙周膜细胞, 蛋白激酶样内质网激酶, 成骨分化, 转录调控因子4

Abstract: PURPOSE: To explore whether RNA-activated protein kinase-like ER resident kinase(PERK) has a regulatory effect on the osteogenic differentiation of human periodontal ligament cells (PDLCs). METHODS: PDLCs were primarily cultured, and siRNA was used to silence the expression of PERK in PDLCs. PDLCs were cultured in osteoblast differentiation-induction medium. Subsequently, the osteogenic differentiation capacity was evaluated using alkaline phosphatase(ALP) staining and alizarin red staining. RESULTS: After osteogenic induction culture of PDLCs in vitro, ALP and alizarin red staining were enhanced, and the deposition of mineral salts increased. Meanwhile, the expression of phosphorylated PERK(p-PERK) in osteogenic PDLCs was higher than cells in the control group. Transfection of si-PERK significantly downregulated PERK protein expression in PDLCs (P<0.05). ALP and alizarin red staining showed that the PERK^-/- group had markedly lower ALP staining intensity and calcium salt deposition than the osteogenic induction group, along with decreased activating transcription factor 4(ATF4) protein expression. CONCLUSIONS: PERK regulates the osteogenic differentiation of PDLCs.

Key words: Human periodontal ligament cells, RNA-activated protein kinase-like ER-resident kinase, Osteogenic differentiation, ATF4

中图分类号:

R781.4

李莉芬, 杜嵘, 江龙. PERK对人牙周膜细胞成骨分化的调控作用[J]. 上海口腔医学, 2025, 34(6): 571-576.

Li Lifen, Du Rong, Jiang Long. The regulation of PERK on osteogenic differentiation of human periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2025, 34(6): 571-576.

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PERK对人牙周膜细胞成骨分化的调控作用

The regulation of PERK on osteogenic differentiation of human periodontal ligament cells

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