上海口腔医学

上海口腔医学 ›› 2025, Vol. 34 ›› Issue (3): 225-232.doi: 10.19439/j.sjos.2025.03.001

• 论著 • 上一篇    下一篇

虾青素静电纺贴片对大鼠口腔黏膜溃疡的治疗效果及潜在机制研究

王欢1, 孙培1,2, 刘昭辰1, 张慧1,2, 郭宜青1,2, 赵厚萍3, 王培彦1, 邓婧1,2   

  1. 1.青岛大学口腔医学院,山东 青岛 266003;
    2.青岛大学附属医院 口腔内科,山东 青岛 266003;
    3.临沂市中心医院,山东 临沂 276400
  • 收稿日期:2024-12-26 修回日期:2025-01-13 出版日期:2025-06-25 发布日期:2025-06-24
  • 通讯作者: 邓婧,E-mail:dengjing3333@126.com
  • 作者简介:王欢（1995-）,女,在读硕士研究生,E-mail：17853573113@163.com
  • 基金资助:
    国家自然科学基金包干制项目（82401111）

Study on the therapeutic effect and potential mechanisms of astaxanthin electrospun patches on oral mucosal ulcers in rats

Wang Huan1, Sun Pei1,2, Liu Zhaochen1, Zhang Hui1,2, Guo Yiqing1,2, Zhao Houping3, Wang Peiyan1, Deng Jing1,2   

  1. 1. School of Stomatology, Qingdao University. Qingdao 266003;
    2. Department of Stomatology, Affiliated Hospital of Qingdao University. Qingdao 266003;
    3. Linyi Central Hospital. Linyi 276400, Shandong Province, China
  • Received:2024-12-26 Revised:2025-01-13 Online:2025-06-25 Published:2025-06-24

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摘要： 目的: 研究虾青素静电纺贴片治疗大鼠口腔溃疡的效果及潜在机制。方法: 用机械创伤法建立大鼠口腔溃疡模型,随机分为虾青素静电纺贴片组、壳聚糖贴片组、甲硝唑贴片组及空白对照组,观察各组溃疡愈合时间和面积愈合率。采用H-E染色观察给药2、4、6天后各组溃疡组织的病理变化并评分。通过WST-1、TBA法检测溃疡组织中超氧化物歧化酶（superoxide dismutase,SOD）和丙二醛（malondialdehyde,MDA）水平变化。通过免疫组织化学实验对肿瘤坏死因子α（tumor necrosis factor-α,TNF-α）、白细胞介素6（interleukin 6,IL-6）进行检测分析。结果: 与其他组相比,虾青素静电纺贴片可显著加快溃疡面积的缩小,促进病理学愈合,降低TNF-α、IL-6表达水平,并显著降低溃疡组织中MDA含量,提高SOD活性。给药6天后,虾青素静电纺贴片组SOD和MDA水平与空白对照组及甲硝唑贴片组相比差异显著（P<0.05）,与壳聚糖贴片组无显著差异（P>0.05）。结论: 虾青素静电纺贴片促进溃疡愈合的性能更优,可能与其强抗氧化及抗炎性能有关,有望成为口腔溃疡治疗效果更佳的天然海洋制剂。

关键词: 虾青素, 口腔溃疡, 抗氧化活性, 炎症因子, 大鼠

Abstract: PURPOSE: To investigate the effect and underlying mechanism of astaxanthin electrospun patches in treating oral ulcers in rats. METHODS: An oral ulcer model of rats was established by mechanical trauma, then the rats were randomly divided into astaxanthin electrospun patch group, chitosan patch group, metronidazole patch group and blank control group. The ulcer healing time and the area healing rate of each group were observed. The pathological changes of ulcer tissues in each group were observed and scored by H-E staining 2, 4 and 6 days after drug administration. Meanwhile, the toxic effects of the drugs on the rat organs were detected. The changes in the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in ulcer tissues were detected by WST-1 and TBA methods. Tumor necrosis factor-α (TNF-α) and Interleukin 6 (IL-6) were analyzed through immunohistochemistry experiments. RESULTS: Compared with the other groups, the astaxanthin electrospun patch could significantly accelerate the reduction of the ulcer area, promote its pathological healing, reduce the expression levels of TNF-α and IL-6, and significantly decrease the content of MDA in the ulcer tissue while increasing the activity of SOD. Six days after administration, there were significant differences in the levels of SOD and MDA compared with the blank control group and the metronidazole patch group(P<0.05), while there was no significant difference compared with the chitosan patch group(P>0.05). CONCLUSIONS: The astaxanthin electrospun patches have better performance in promoting ulcer healing, which may be related to their strong antioxidant and anti-inflammatory properties. It is expected to provide a natural marine preparation with better efficacy for the treatment of oral ulcers.

Key words: Astaxanthin, Oral ulcers, Antioxidant activity, Inflammatory factor, Rat

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